EMAGE mouse gene expression spatial database

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EMAGE:17411

Lman2l lectin, mannose-binding 2-like ( MGI:2443010)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003853. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003853_01	euxassay_003853_02	euxassay_003853_03	euxassay_003853_04

euxassay_003853_05	euxassay_003853_06	euxassay_003853_07	euxassay_003853_08	euxassay_003853_09

euxassay_003853_10	euxassay_003853_11	euxassay_003853_12	euxassay_003853_13	euxassay_003853_14

euxassay_003853_15	euxassay_003853_16	euxassay_003853_17	euxassay_003853_18	euxassay_003853_19

euxassay_003853_20	euxassay_003853_21	euxassay_003853_22	euxassay_003853_23	euxassay_003853_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17411_wholemount_strong.wlz

17411_wholemount_moderate.wlz

17411_wholemount_weak.wlz

17411_wholemount_possible.wlz

17411_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17411_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7779
Entity Detected:	Lman2l, lectin, mannose-binding 2-like ( MGI:2443010)
Sequence:	sense strand is shown >T7779 GGAAAGATGGCGGCGGCTTCGAGACCCTCCTGGTGGCAGCGGTGGCGGCGGCGCGCGTGGGCTCGGGACG GGGCCAAGCTTTTGCTCTTTCTCCTTCTGTTGGGGTCTGGGCCGGGGCCGCGGCATGTCAGGGCGGGTCA AGCGGTGGAGTACTTGAAACGGGAGCACTCGCTGTCGAAGCCCTACCAGGGTGTGGGAACAAGCAGTTCT TCGCTATGGAACCTGATGGGCAACGCCATGGTGATGACACAGTACATCCGCCTCACTCCAGATATGCAAA GTAAACAGGGTGCCTTGTGGAACCGGGTGCCATGTTTCCTGAAAGACTGGGAACTGCAGGTGCACTTCAA GATTCACGGCCAAGGGAAGAAGAATCTGCACGGAGACGGCTTGGCAATCTGGTACACAAAGGACCGAATG CAGCCAGGGCCTGTGTTTGGAAACATGGACAAATTTGTGGGGCTGGGAGTGTTTGTAGACACCTATCCCA ATGAGGAGAAGCAGCACGAGCGGGTATTCCCCTACATCTCAGCCATGGTGAACAACGGCTCCCTCAGCTA TGATCACGAGCGGGATGGGCGGCCTACAGAGCTGGGTGGCTGCACAGCCATTGTCCGAAATATCCGTTAT GACACCTTCCTCGTGATTCGCTATGTCAAGAGGCATCTGACGATAATGATGGATATTGATGGCAAACATG AGTGGAGGGACTGCATTGAGATGCCCGGGGTCCGGCTCCCCCGGGGCTATTACTTCGGCACTTCCTCCAT TACTGGGGATCTCTCTGATAACCATGATGTCATTTCCCTCAAGTTGTTTGAGCTGACTGGAGTGAGGACC CCAGAAGAAGAAAAGCTACATCGAGATGTATTCCTGCCCTCGGTGGACAATCTGAAGCTGCCAGAGATGA CAGTGCCACCAACGCCCCTGAGTGGCCTGGCTCTCTTCCTTATTGTCTTCTTCTCCTTGGTGTTTTCCGT ATTTGCCATTGTCATCGGGATCATACTCTACAACAAATGGCAAGACCAGAGCCGGAAACGCTTCTACTGA GCCCGCTGCCATCAGTCCCTCTACGGTGTTTACCATAAGGTGTGGGTCTGAGCATACAGCCTAGCGAGTC GGCCCATTGTTCAGGGACTGTGTTCTGTCACTGGAGATTTGAATGCTAGGAGCCTGCATCCTCATGGTCA TCCACGGGAACATCTCACTGGCCCAGGATACCACCACAGGGCCGAGCAGCTGTGGCATGCCTTTCCCTGG AGTCCTTCCATTTCCGAGTACAGAGACATGGAGAAATACCTGCTGTGATGCCTAAATTACACAATTGGGC AGATAGCCTAAGTGTGGACTGGACACAGTAGGCCTTCCATTGCATTTTAGAATTCCCAAATAGGAAACCA GAACCGGTACACTCTGGAGACCCACTTACTGGTCTTTGCTTTGCCCATGCCTCTTTCTACTTCAGGAGCT CCTTGGCAATCTGGATGGAGACTTCTCCCTCACCCGCCTGTCTCCCTCCACTCACCGTCCTCTTTATATG CATCCTTAGCTAGGAAAGAAGTTTGCCGCTCTCATTAGCTGGACCTGGGGCTTCCTGCAGCCTCTCATTG CTTCAGCCTCTTCTCTGCTGGGTGGATCCTGTTTCTGACTGTGGCTGTGTCTTCTGCTTTCCTCATCAAA GGAAACCTTCAGGCCTTTGGATGGTTTGGCTAAAAGTTGTTACAACTCAAGAGAAGCTTGGAGGAGTTGA CAGATGCTGTGAGGTTAACTCTGGAATCATCCAGGTCCAGGTGTGCCTCAGAGAGCAGTGTTGGCTGCGT CTGGACCTGCTGGAGCCTGCTTAGTCGCATGTGTTGTGTTTGTCCTGATTCTGCTCTGAGTGTTGGAAGT GATAAGCAGCTTTCTTCTTACTCCCTGGACTTGGACATGGCCCCAAGATGCTTGGCCTCTTACTAAGAAG AAACAGTTGGTAATCTGTTACACATCTCTGAGAGTGAAAGCCCTTCATCCTCTGTGCCTGGAAAAGTTGT GGTCCTGGAGCAGCAGCACAGCTTGAGTGCCCTTGTGTGTCACCTATTACACTGCCTTATTCCACAAGGG CCACATGCTGCTCACCTATCTTCTCTGTGGTTAAATTGGTCACAGGCTAGTCTCCCTGGAGGGCCTGGCA CAGTTCTCTGTAGCCCGAGGAAAAGGCTTCATAAAGTAGCTGATGGAACCACTCAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6313679 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6313679 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17412 same embryo
	EMAGE:17410 same embryo
	EMAGE:17413 same embryo
	EurExpress:euxassay_003853 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source