EMAGE mouse gene expression spatial database

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EMAGE:17415

E2f1 E2F transcription factor 1 ( MGI:101941)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003850. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003850_01	euxassay_003850_02	euxassay_003850_03	euxassay_003850_04

euxassay_003850_05	euxassay_003850_06	euxassay_003850_07	euxassay_003850_08	euxassay_003850_09

euxassay_003850_10	euxassay_003850_11	euxassay_003850_12	euxassay_003850_13	euxassay_003850_14

euxassay_003850_15	euxassay_003850_16	euxassay_003850_17	euxassay_003850_18	euxassay_003850_19

euxassay_003850_20	euxassay_003850_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17415_wholemount_strong.wlz

17415_wholemount_moderate.wlz

17415_wholemount_weak.wlz

17415_wholemount_possible.wlz

17415_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17415_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7804
Entity Detected:	E2f1, E2F transcription factor 1 ( MGI:101941)
Sequence:	sense strand is shown >T7804 GCCGGGGCGGAGCGGGATCGAGCCCTCGCCGCGGCCTGCCAGTCATGGGCCCGCGCCGCCGCCGCCGCCT GCCTCCCGGGCCACGCGGGCCGTGAGCGCCATGGCCGTAGCCCCCGCGGGCGGCCAGCACGCGCCAGCGC TGGAGGCCCTGCTCGGGGCGGGCGCGTTGCGGCTGCTCGACTCCTCGCAGATCGTCATCATCTCCACCGC GCCCGATGTCGGCGCCCCGCAGCTCCCCGCCGCGCCGCCCACTGGCCCTCGCGATTCTGACGTGCTGCTC TTCGCCACGCCGCAGGCGCCCCGACCCGCGCCTAGTGCACCGCGCCCGGCTCTCGGCCGCCCGCCGGTGA AACGGAGGCTGGATCTGGAGACTGACCATCAGTACCTCGCTGGTAGCAGTGGGCCATTCCGGGGCAGAGG CCGCCACCCAGGGAAAGGTGTGAAATCTCCGGGGGAGAAGTCACGCTATGAAACCTCACTAAATCTGACC ACCAAACGCTTCTTGGAGCTGCTGAGCCGCTCAGCTGACGGTGTCGTTGACCTGAACTGGGCAGCTGAGG TGCTGAAGGTGCAGAAACGGCGCATCTATGACATCACCAATGTCCTGGAGGGCATCCAGCTCATTGCCAA GAAGTCCAAGAATCATATCCAGTGGCTAGGCAGCCACACCATGGTGGGGATTGGTAAGCGGCTTGAAGGC CTGACCCAGGACCTGCAGCAACTGCAGGAGAGTGAGCAGCAGCTGGATCACCTGATGCACATCTGTACCA CACAGCTGCAACTGCTTTCGGAGGACTCCGACACCCAGCGCCTGGCCTATGTGACCTGCCAGGACCTTCG CAGCATTGCAGACCCTGCAGAACAGATGGTCATAGTGATCAAGGCCCCTCCTGAGACCCAACTACAAGCT GTGGATTCTTCAGAGACATTTCAGATCTCCCTTAAGAGCAAACAAGGCCCCATTGATGTTTTCCTGTGCC CGGAGGAGAGTGCAGACGGGATTAGCCCTGGGAAGACCTCATGCCAGGAGACATCCTCTGGGGAGGACCG GACTGCAGACTCTGGCCCAGCAGGGCCTCCACCATCACCTCCCTCCACATCCCCAGCCTTGGATCCCAGT CAATCCCTGTTGGGCCTGGAGCAAGAAGCAGTATTGCCACGGATGGGCCACCTGAGGGTCCCTATGGAAG AGGACCAACTGTCACCACTGGTGGCTGCTGACTCACTCCTGGAGCATGTTAAAGAAGACTTCTCTGGGCT CCTCCCTGGGGAGTTCATCAGCCTCTCCCCACCCCACGAGGCCCTTGACTATCACTTTGGTCTCGAGGAG GGTGAGGGCATTAGAGATCTCTTTGACTGTGACTTTGGGGACCTGACCCCTCTGGATTTCTGACAGAAGC CTAGGGATTCAGGGTGTCTGGAGATGCCCACCTGTCTGCAGCTTTGGAGCCTCCTGCCCTGGGCCATCCT TCCTGCCTCATTGGAATAGCACGATCCATACCCTCTGTCCCAATAGCTTCTAGCTCTGGGGTTTGGTTGC TGCCACATTGAGCAGACCAAAATGGGAAGGATGTTGTACAGTGTGTGTGCATGCACCCCACACTGCGCAC TGTGTGCCTGGGGTGTGTGTCTGAGTGTGTGTGTGTGTGTGTGTGTGTGAGTGTGTGTGTGTGTGTGTGT GAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTATGTGTATGTGCACGTGT GCCCGGGAATGAAGGTGAACACATCTGTATGTGTGCTGCAGACACATCCTGGTGTGTCCACATGTGTGCA TGGATCCATGTGTGCGCATTGGGGTGGGGGTGGGCTCTAACTGCACTTTTGGTGTCCTTGCTGCAGGGGC CCTGTGAGGCCCAGGGTGGCTGCCTGCTTTCAGAATCCTGTGTGTCAGCCAGGCCGGGTGGTACAGCTTG CCTGGCTGGGTTTGCAGGGCAGCAAGAGCCCTGCTTAAAAGTTTTCCGATCGGAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6330425 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6330425 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17416 same embryo
	EMAGE:17414 same embryo
	EMAGE:17417 same embryo
	EurExpress:euxassay_003850 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source