EMAGE mouse gene expression spatial database

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EMAGE:17416

Alkbh8 alkB, alkylation repair homolog 8 (E. coli) ( MGI:1914917)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003844. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003844_01	euxassay_003844_02	euxassay_003844_03	euxassay_003844_04

euxassay_003844_05	euxassay_003844_06	euxassay_003844_07	euxassay_003844_08	euxassay_003844_09

euxassay_003844_10	euxassay_003844_11	euxassay_003844_12	euxassay_003844_13	euxassay_003844_14

euxassay_003844_15	euxassay_003844_16	euxassay_003844_17	euxassay_003844_18	euxassay_003844_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17416_wholemount_strong.wlz

17416_wholemount_moderate.wlz

17416_wholemount_weak.wlz

17416_wholemount_possible.wlz

17416_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17416_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 03 14

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 13 14 15 16 17 18

vagus x ganglion

moderate

regional

moderate expression: see section 13

spinal cord

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 05 06 11

cervical ganglion

moderate

regional

moderate expression: see section 04 12

thoracic ganglion

moderate

regional

moderate expression: see section 06 07 08 09

dorsal root ganglion

moderate

regional

moderate expression: see section 02 03 04 05 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7802
Entity Detected:	Alkbh8, alkB, alkylation repair homolog 8 (E. coli) ( MGI:1914917)
Sequence:	sense strand is shown >T7802 GATTGGTGTGGTTTACACAGTATTACTATGAATATCAATCATAAAGGTGTTTTGAAACTCACTAAAATGG AAAAGAAGTTCTTAAGGAAACAGAGTAAAGCCAGGCATGTGTTGCTGAAACATGAAGGCATCCAAGCAGT GTCTTATCCCACTCAGAGCCTGGTTATTGCCAATGGTGGTCTGGGTAATGGCGTGAGTAGGAAACAACTG CTTTTGACTTTGGAGAAATGTGGACCTGTGGAAGCTCTCCTCATGCCACCTAATAAGCCATATGCATTTG TAATATTCCAAACTATTGAAGAATCCAAGAAAGCATATTTTACCCTCAATGGAAAAGAAATCATTGATGA TTTGGGGCAGAAGATCTTTCTGTACTTGAATTTTGTTGAAAAAGCACAATGGAAAAACATGGGCCTTGAA GCCTTACCTCCAGGCCTCTTGGTGGTAGAAGAAATTATTTCTTCTGAGGAGGAGAAAAAGCTTCTGGAGA GTGTGAATTGGACAGAAGATACAGGCAATCAGAATTTTCAAAGGTCCTTAAAACACAGAAGAGTGAAACA TTTTGGCTATGAATTTCACTATGAGAGCAACACTGTGGATAAAGACAAGCCCTTACCTGGGGGTCTTCCT GAGGTTTGCAGTAGCATTTTGGAGAAGTTGTTGAAAGAAGGTTATATTAAACATAAACCAGACCAGTTGA CCATAAATCAATATGAACCTGGGCATGGAATTCCTGCCCATATTGACACGCATTCTGCATTTGAAGATGA GATTATTTCTCTCAGTTTGGGGTCAGCGATTGTTATGGATTTCAAGCATCCAGAGGGTGTCACAGTACAA GTTATGTTACCTCGTCGGAGTTTGCTGGTGATGACAGGAGAATCTAGATACCTTTGGACTCATGGAATTA CACCTCGAAAATTTGATACCGTCCAAGCGTCTGAGCAATTTAAAGGGGGAATAATCACCAGTGACATTGG AGACTTGACTTTAAGCAAGAGGGGAATGCGCACATCCTTTACGTTTAGAAAAGTGAGGCGAATGCCCTGC AATTGTAGTTACTCCTCAGTCTGCGACAGACAAAGGAAGGCCACACCTCCATCCCTTACAGAGAGCAGTA AAGAAGCATTGGAGTTAGAGCAAAAGCATGTCCATCAAGTGTACAATGAGATTGCAAGTCACTTCAGCAG CACAAGACATAGCCCATGGCCACGCATAGTAGAGTTCCTGAAAGCTTTGCCAAGTGGTTCCATAGTGGCA GATATCGGATGTGGAAATGGAAAATACCTTGGCATCAACAAGGACTTATATATGATTGGGTGTGATCGTA GCCAGAACCTTGTGGACATTTGTAGAGAGAGGCAGTTCCAGGCCTTAGTCTGCGATGCGCTGGCTGTTCC TGTCAGAAGTGGGTCTTGTGATGCTTGCATCTCTATTGCCGTCATTCACCACTTTGCAACTGCAGAGCGC AGAGTGGAAGCTCTCCAAGAACTTGCCCGTCTCCTGAGACCTGGTGGGCAGGCCCTCATTTATGTCTGGG CAATGGAACAAGAGTATAAAAATCAGAAGTCCAAGTATCTTAGAGGAAAGAGAATTAGCCAAGGAGATAA AGATGAGCTAAACAGTGCCACATCTACGGAAGAATTTCTAGTAAATCAAACGCCTGAGGGGGTCAATGAA GACCCAGCATTGTCTGTCAACTCCAGTAGTATCACCAAGGAAGAAGAGTATAAGTCAAGGAAAGTTCCTA ATTCTGAACTCCCTATTCATATAAATAGGACCTGCTTTCATTCCCAAGATGTGCTGGTTCCCTGGCACCT CAAAAGAAACCCTGGTAAAGACAAAGCTATTGAACCATCTGGTGTAGCAGGATGTCCTGACCCAAGTCCT GTGTTTCATCGTTATTACCATGTTTTCTGTGATGGAGAATTGGAAGCTTCATGCCAGGCTGTGGGTGATG TTAGCATTCTGCAGAGCTATTACGATCAAGGGAATTGGTGTGTTGTTCTTCAAAAGGTCTGATCACTTAT CTGAACATATCATGTGTAAAGAGAAAAAAAAAAGTCAATCTCTTTTTGAAAAGGAAACTAAATTATCTCT TTAAATAAGGAGGAAATTTGGAACAGAGATACTTAGGAAACATTTTTCTCTATTCCCGTTGTCTGACTTC ATAAACGTAGGTTCCTCTTACATGCCTTTTAAGTATGGTGTTTGACATTAACTTGTGAAAAGTCTGTGAT GTTTGTATATATATAGCATTTGAAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6329366 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6329366 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17414 same embryo
	EMAGE:17415 same embryo
	EMAGE:17417 same embryo
	EurExpress:euxassay_003844 same experiment
	MGI:4823090 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source