EMAGE mouse gene expression spatial database

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EMAGE:17421

2810030E01Rik RIKEN cDNA 2810030E01 gene ( MGI:1919918)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003843. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003843_01	euxassay_003843_02	euxassay_003843_03	euxassay_003843_04

euxassay_003843_05	euxassay_003843_06	euxassay_003843_07	euxassay_003843_08	euxassay_003843_09

euxassay_003843_10	euxassay_003843_11	euxassay_003843_12	euxassay_003843_13	euxassay_003843_14

euxassay_003843_15	euxassay_003843_16	euxassay_003843_17	euxassay_003843_18	euxassay_003843_19

euxassay_003843_20	euxassay_003843_21	euxassay_003843_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17421_wholemount_strong.wlz

17421_wholemount_moderate.wlz

17421_wholemount_weak.wlz

17421_wholemount_possible.wlz

17421_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17421_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

body cavity or lining

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

limb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 19 20 21 22

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

gland

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

visceral organ system

moderate

regional

moderate expression: see section 05 18

alimentary system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

nervous system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

integumental system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

sensory organ system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

cardiovascular system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

urinary system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

reproductive system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

respiratory system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

skeleton

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

tail

moderate

regional

moderate expression: see section 06 10 11 12 13 14 15 16 17 not examined expression: see section 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7808
Entity Detected:	2810030E01Rik, RIKEN cDNA 2810030E01 gene ( MGI:1919918)
Sequence:	sense strand is shown >T7808 ATGAAGTCTGATGCCTTGAGACACACGCAGCATCTGGCGGAGGATTAACATACATACATGTGTATGTATA CGTCACGTATATATTTACTGCAAATGGTGGGGATCATTTAGTGCCCGAGATGGGAGACCTGAAGTCAGGT TTCGAAGAGGTGGATGGCGTGAGGCTCGGCTACCTCATCATTAAAGGGAAGCAAATGTTTGCTCTCTCCC AAGTCTTCACAGATCTGCTGAAAAACATCCCGAGGACGACCGTGCACAAGCGCATGGATCATCTGAAAGT GAAAAAGCACCACTGCGATCTGGAGGAGTTGCGGAAACTCAAAGCAATCAACAGTATCGCCTTCCACGCG GCCAAATGCACGCTCATCTCCCGGGAAGACGTAGAAGCGCTCTACACATCCTGCAAAACCGAGCGTGTGC TCAAGACCAAGCGAAGGAGAGTCGGTCGGGCCCTGGCCACAAAGGCGCCGCCGCCAGAGCGCGCCGCCGC CGCCAGCCCCCGCCCGGCTTTTTGGAAGGACAAGCACCAACTTTGGCGGGGCCTGAGCGGAGCCGCGCGG CCCCTGCCAATCAGCGCGCAGTCCCAGCGTCCGGGCGCCGCCGCCGCGCGCCCCGCCGCCCATCTACCTC AGATTTTTAGCAAATACCCGGGCTCCCACTACCCGGAGATCGTTCGCTCGCCTTGCAAATCCTCTTTAAA CTATGAAACTGCCCAGCTCCAGGGAAACTACGTCGCCTTCCACTCGGACCCCGCTTATTTTCGGAGCCTG CTGTGCAGCAAGCACCCGGCGGCGGCCGGGGCCACTTGCTTGGAGAGGTTTCATCTGGTCAACAGCTTCT GCCCGCCTCCCCACCACCACCATCACCATCACCACCATCATCATCACCATCACCACCGAGCCCAGCAGCC AACGCCGAGTCACCACCCCTCTCATCACCACCGGCCGCAACCCCATCTAGGAAGCTTTCCCGAGAGTTGT AGCAGCGACTCCGAGTCCAGCTCCTACTCCGACCATGCAGCCAATGACTCGGATTTTGGCTCCAGTTTGT CCAGCTCCAGCAACTCGGTGTCCTCAGAGGAAGAGGAGGAGGAAGGAGAGGAGGAGGAGGAAGAGGAGGA GGAGGAAGAGGGGGGCAGCGGGGCCTCGGATTCCAGTGAAATCAGCTCGGAGGAGGAGGATTCTTCCACC GAGTCAGACTCCAGCTCCGGGTCCAGCCAAGTGTCAGTGCAGAGCATCCGTTTCAGGCGCACCAGCTTCT GCAAGCCTCCCAGCGTGCAGGCGCAGGCCAACTTCTTGTACCATCTGGCCTCCGCCGCCGCTGCAACCAA ACCCGCTGCTTTCGAGGATGCCGGCCGACTTCCCGACCTCAAGAGTAGTGTCAAAGCCGAGTCACCAGAG GAGTGGAGTCTGCAGAGCTGGGCTCCCAAAGGGACTCCGGTGTACTGCCCGGCCAGCTTGGGGAGTTGTT TCCCAGAGATAAGAAACGATAGGGTATCTGAGATTACATTCCCACACTCTGAAATATCCAGTACTGTAAA GAGAACTGACCTGACAATTAACTGCCATGCAGAGGGGGCCTCTTCACCTAGCCCAAAGACAAACAATGTA TTTCCACAACAAAGAATACTCCGAGAGGCTAGGAAATGCCTACAAGCAACTCCTACTACACACTGTGCAG AAAACAGCACAATAGCTGCTAGGTTCTTAAATAATGATTCGTGTGGAACAACAGCAAATTCAGGAAAAGA TTCCAAAATCCCTCATTGTCCTGAATTTGCTACGGATTTACCCTCTCTTCAAAGTGATCCCAGAGTGGAT ACAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAACTAAAGCTGAGAGTCTCTGCACTGGTACAGGTGACA AGACATTGCCATTTCTGCACAATATTAAAATCAAAGTAGAAGACAGTAGTGCTAATGAAGAATATGAACC TGAGCTTATTACAAATAAGCTAAAGTGGGAGTGCAATGATGCCGAGGGTGAGTTTTACAATATGACTGAG AAGAAGGAGGAGGACGCCTTGGTACCCACAGCCAAGGAAGGTTTTGCATGCCCTGAGAAAGAAACGCCTT CCTTAAATCCACTGGCTCAGAGTCAGGGCCTCTCATGCACTTTAGGATCTCCAAAACCTGAGGATGGGGA ATATAAATTTGGTGCCAGGGTGAGAAAAAATTACCGGACACTAGTACTGGGCAAACGACCAGTCCTTCAG ACACCTCCAGTCAAACCAAATTTGAAATCAGCTAGAAGCCCTCGTCCTACAGGTAAAACTGAGACACATG AAGGAACACTGGATGACTTTACAGTTTTAAACAGACGCAAAAAGGTAGCCAGCAATGTAGCATCAGCAGT GAAAAGGCCATTTAATTTCATGGCAAATTTTCCTTGTCCACCTTCACTCATTATTGGGAAGGATGGGGAT TTGTGGCCAGCGTATTCCTTAAACACCACTAAGGATTCCCAACCTCCTCACAAGGCCCATCCTATATGGA AATGGCAGCTGGGCGGTTCTGCAATACCTCTTCCACCTAGTCACAAATTCAGGAAATTTAATTCATAAAA AAATGTTTTGGAAGATATTTTCTCGAACCATATTACCTTCCTTTTGTTGTAAACTGCACAGGATGGCTTG TACCAAGTCCATTAATGGTGTTACACCCCTTTTGGAGTCCTGGTTTATCGCATTTTGAAGACAAAAATCG GATTACTTTTTTTTCCCATTGCGGGTTGTTTTTTTTTTTTTTTTATGGTGGGGGAGGGGTGGGAGAAGGG TTGGTTTACATACCACAGACTACTTCAGGCTAAAGACTCAAGTAACACTCAGTTATTATGGTAGAGCTGG AACACTTTACTGTTTCAATGGTAATAATGCACCGGTACCTCAATTCAACTGCTACAAATAAATGTCAAAA GGTTGAATAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6331376 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6331376 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17418 same embryo
	EMAGE:17420 same embryo
	EMAGE:17419 same embryo
	EurExpress:euxassay_003843 same experiment
	MGI:4822686 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source