EMAGE mouse gene expression spatial database

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EMAGE:17448

Satb1 special AT-rich sequence binding protein 1 ( MGI:105084)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003888. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003888_01	euxassay_003888_02	euxassay_003888_03	euxassay_003888_04

euxassay_003888_05	euxassay_003888_06	euxassay_003888_07	euxassay_003888_08	euxassay_003888_09

euxassay_003888_10	euxassay_003888_11	euxassay_003888_12	euxassay_003888_13	euxassay_003888_14

euxassay_003888_15	euxassay_003888_16	euxassay_003888_17	euxassay_003888_18	euxassay_003888_19

euxassay_003888_20	euxassay_003888_21	euxassay_003888_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17448_wholemount_strong.wlz

17448_wholemount_moderate.wlz

17448_wholemount_weak.wlz

17448_wholemount_possible.wlz

17448_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17448_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

homogeneous

weak expression: see section 11 12 13

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

pons marginal layer

strong

regional

strong expression: see section 10 13 14 moderate expression: see section 09

facial vii ganglion

weak

regional

weak expression: see section 04 18 19

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 16

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 15 16 17 18 19 20

vagus x ganglion

weak

regional

weak expression: see section 08 09 15

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 07 08 09 15 16 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 13

cervical ganglion

moderate

regional

moderate expression: see section 09 14 15

thoracic ganglion

moderate

regional

moderate expression: see section 10

dorsal root ganglion

weak

regional

weak expression: see section 07 08 09 11 12 13 14

neural retina

weak

homogeneous

weak expression: see section 01 02 03 04 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7263
Entity Detected:	Satb1, special AT-rich sequence binding protein 1 ( MGI:105084)
Sequence:	sense strand is shown >T7263 GCGGCGGGCGGCGAGGGTGCGCGGCGGGGGCGGTGCAGTTCTGGCAGGTGATCTGTAAGACAGTGACTGA GTATGGATCATTTGAACGAGGCAACTCAGGGGAAAGAACATTCAGAAATGTCTAACAATGTGAGTGATCC GAAGGGTCCACCCGCCAAGATTGCCCGCCTGGAGCAGAACGGGAGCCCTCTAGGAAGAGGAAGGCTTGGG AGCACAGGTGGAAAGATGCAGGGAGTGCCTTTAAAACACTCGGGCCATCTCATGAAAACCAACCTTAGGA AAGGAACCATGTTACCAGTTTTCTGCGTGGTGGAACATTATGAAAACGCCATTGAGTATGATTGCAAGGA GGAGCACGCGGAATTTGTATTGGTGAGAAAGGATATGCTTTTCAACCAGCTGATAGAGATGGCGTTGCTG TCTCTAGGCTATTCACACAGCTCTGCTGCCCAAGCCAAAGGGCTCATCCAGGTTGGGAAGTGGAATCCAG TTCCACTGTCGTATGTGACAGATGCCCCTGATGCCACGGTGGCAGACATGCTTCAAGATGTGTATCATGT GGTCACCCTCAAAATTCAGTTACACAGTTGCCCTAAACTAGAAGACTTGCCTCCTGAACAATGGTCGCAC ACCACAGTAAGGAATGCTCTGAAGGACTTACTGAAAGATATGAACCAGAGTTCGTTGGCCAAGGAGTGCC CCCTTTCACAGAGCATGATCTCCTCCATTGTGAACAGCACGTACTATGCAAATGTCTCAGCAGCAAAATG TCAAGAATTTGGAAGGTGGTACAAACATTTCAAGAAGACAAAGGATATGATGGTTGAGATGGATAGTCTG TCTGAACTATCCCAGCAAGGTGCCAACCACGTCAATTTTGGCCAGCAGCCTGTCCCAGGAAACACAGCTG AGCAGCCTCCATCCCCTGCGCAGCTCTCTCACGGCAGTCAGCCCTCTGTCCGGACCCCTCTTCCGAACCT GCACCCTGGGCTTGTGTCAACACCGATCAGTCCTCAGCTGGTCAACCAACAGCTGGTGATGGCTCAGTTG CTGAACCAGCAGTATGCAGTGAACAGACTCTTAGCCCAGCAGTCCTTAAACCAACAGTACTTGAACCACC CTCCCCCTGTCAGTAGGTCTATGAACAAGCCTTTGGAGCAGCAAGTTTCCACGAACACGGAGGTCTCTTC TGAAATCTACCAGTGGGTGCGGGATGAACTGAAACGAGCCGGAATCTCACAGGCAGTATTTGCACGCGTG GCTTTTAACCGAACTCAGGGATTGCTTTCTGAAATCCTCCGAAAGGAAGAGGACCCCAAGACTGCATCCC AGTCTCTGCTGGTAAACCTTCGGGCTATGCAGAATTTCTTACAGTTGCCGGAAGCCGAAAGAGACCGGAT ATACCAGGATGAGAGGGAAAGGAGCTTGAACGCAGCCTCAGCCATGGGTCCTGCCCCGCTGCTGAGCACA CCACCCAGCCGCCCTCCCCAGGTGAAAACAGCTACCCTTGCCACTGAGAGAAATGGGAAGCCAGAGAACA ATACTATGAACATTAATGCCTCCATTTATGACGAGATTCAGCAGGAAATGAAGCGTGCTAAAGTGTCCCA AGCACTGTTTGCAAAGGTTGCCGCCACAAAAAGCCAGGGATGGCTGTGTGAGCTGTTGCGCTGGAAAGAA GATCCTTCTCCAGAAAACAGGACCCTGTGGGAGAACCTGTCGATGATCCGAAGATTCCTCAGTCTGCCCC AGCCTGAGCGCGATGCCATCTATGAGCAGGAGAGCAATGCTGTGCATCACCATGGCGACAGACCTCCCCA CATCATCCACGTTCCAGCAGAACAGATTCAGCAGCAGCAACAGCAGCAGCAGCAACAGCAGCAGCAGCAG CAGCCACCGCCGCCACCGCCGCAGCCACAGCCACAGCCCCAGGCAGGCCCCAGACTCCCCCCACGGCAGC CCACCGTGGCCTCCTCCGCGGAGTCCGATGAGGAAAACCGGCAGAAGACCAGGCCACGAACCAAAATTTC CGTGGAAGCCCTGGGGATCCTTCAGAGTTTCATCCAAGATGTGGGCCTGTACCCAGATGAAGAGGCTATC CAGACTCTGTCTGCACAGCTGGACCTCCCGAAGTACACCATCATCAAGTTCTTTCAGAACCAGCGGTACT ACCTTAAGCACCATGGCAAGCTGAAGGACAACTCCGGCTTGGAGGTGGATGTGGCCGAGTACAAAGATGA GGAGTTGCTTAAGGATTTGGAAGAGAGCGTCCAGGATAAAAACGCCAACACCCTTTTCTCAGTGAAACTA GAGGAAGAGCTGTCGGTGGAAGGGAGCACAGACGTTAATGCCGACTTGAAAGACTGAGAGAACAGTATTC TTTTCAGCCACACCACCGGTATTTCCTAACAACATGAGAAGTCCACCTTGTGTTCACTCAGACAAACCTT CATTGTTTATTGTTTGGCCAATGAATCTTCGGAAACTTGCACAAACAGGAAGGAAGTTGGAAGGACAGGA CAGCCAGCACTCAAGGTTTTACTGTGTTTTACAAAACTGCTTGGCAGCCCCGGGTGAAGCGTCAAGGACT GTTTGGTAGAATTTGTGTTCAAGGGCTGCACCCAGGTGTTGTACCCTGTCAGCATGATACCAGAAATTGG TTTTCCTTTTGATTATTATTATTCTGGAGCCTCAAATAAGCATTATAACTTCTGTGGTTATGATTGCCTT TTCTTTAGTAATTCTTCTGTAACCCACCACACTAGTCTTTGGAAACTGGCCCCTTATTTTAAAGAGAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4164993 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4164993 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17447 same embryo
	EMAGE:17445 same embryo
	EMAGE:17446 same embryo
	EMAGE:17444 same embryo
	EurExpress:euxassay_003888 same experiment
	MGI:4827876 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source