EMAGE mouse gene expression spatial database

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EMAGE:17460

Krt23 keratin 23 ( MGI:2148866)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003899. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003899_01	euxassay_003899_02	euxassay_003899_03	euxassay_003899_04

euxassay_003899_05	euxassay_003899_06	euxassay_003899_07	euxassay_003899_08	euxassay_003899_09

euxassay_003899_10	euxassay_003899_11	euxassay_003899_12	euxassay_003899_13	euxassay_003899_14

euxassay_003899_15	euxassay_003899_16	euxassay_003899_17	euxassay_003899_18

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17460_wholemount_strong.wlz

17460_wholemount_moderate.wlz

17460_wholemount_weak.wlz

17460_wholemount_possible.wlz

17460_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17460_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 07 12 13 14

naris

strong

regional

strong expression: see section 11 15

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 12 13 14 15 moderate expression: see section 16

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 11 12 13 14

metanephros

strong

spotted

strong expression: see section 05 06 07 10 11 12 13 14

lung

moderate

regional

moderate expression: see section 03 04 05 06 10 weak expression: see section 07 08 09 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7304
Entity Detected:	Krt23, keratin 23 ( MGI:2148866)
Sequence:	sense strand is shown >T7304 AGAGAGCTGAGATAGCCCTGCTCCATACACAGATGGGACCATGAACTCCAGCCACAGCTTTAACCAGACC TACTCGGCCTCTGTCCACAGCCTCGGAAGCACCCGGGGGCGGCAGGGGAGCTGCCATCGGGCACCGAGTG TCCATGGAGGCGCAGGGGGTGTCCGCATCTCCCTTTCCTTCACCACGCCAGGCTGCCTGCCTCCTGGAGG ATCATGGGGGTCTGGAAGAAGCAGTCCCCTGCTAGGTGGCAATGGCAAGGCCACCATGCAGAATCTCAAC GATCGCCTGGCCACATATCTGGAGAAGGTGCGGGCTCTAGAGGAGGCTAACTCGAAGCTGGAAACCCGTA TACTAAGGTGGCATCAGGAGAGAGAACCCAGCCACAGAAAGGATTATTCCCAGTATGAGGAAAACATCAG CCGCCTGCAGGAGCAGATAGTGGACGGTAAGATGGCCAATGCCCACATTGTCGTGCTCATTGACAACGCC AGGATGGCAGTGGATGACTTCAACCTGAAGTTTGAAAACGAACACTCGTTAAAGAAAGACTTGGAAATTG AAGTTGAGGGCCTCCGAAAGACCTTGGATGATCTGACCATTGTCACAACAGACCTGGAACAGGAGGTGGA GGGGATGAGGAAAGAGCTGATCCTCATGAAGAAACGCCATGAGCAGGAAATGGAGGAGAATCACTTGCCG AGTGACTTCAAGGTCAGTGTGAAGGTGGACACTACTCCAGGGGAAGATCTGATTAAGGTCTTGGAAGATA TGAGGCAGGAATACGAGTTAATAATAAAGAAGAAACATCAAGAGTTGGACACCTGGTTCAGAGAGCAGTC AGCCGCCATGGCCCAGGAAGTGGCCAGTCCAGCGCCTGTACAGGGCAACCAAAGTGATATCCATGAGCTG AGGAGAACATTCCAGGCCCTAGAGATTGACCTGCAGGCCCAGCACAGCAGGAAAACCGCTTTGGAAAACA TGCTGACAGAGACCCGGGCTCGATACTCCTGCCGTCTCCAGGACATGCAGCAGATCATCTCCCACTACGA AGAAGAGCTGATTCAGCTCCGCCAAGACCTGGAGCGTCAGAACAATGAACACAAGGTGCTGCTGGGCATC AAAACCCACCTGGAGAAGGAAATCGCCACCTACCGCCGGCTCCTGGAGGGAGACACTGAAGGGACGATGG ATGGATCTGAGTCAAGGCTGAAAGGATCTGAAGCTTCAACGATCAAGGCCATCACTCAAGAGAGTGTCAA TGGAAGAATCGTTCTTTCTCAAGTGAATGAGATCCAAAAGCATATATGAAGCCAAGGAGTTTCTGCCTGT TGTAAAAACCACACTCCCCCTATGGAAAGTCTTTGTCTCCGCGCTAATGGGTGAGTCTCGACGGAAATCC TTGGAGTTTTCAGATTCAGAAACTTTTCTCTATAATGGTCTCACAGGACTTCCCATGATGCTCTTAATAT ATTGTTGGAATTTTTGAGTGAAAGAAGAAGTTTATAAATTAAAGCTCTACTCTCCTGAAAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4187035 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4187035 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17461 same embryo
	EMAGE:17458 same embryo
	EMAGE:17457 same embryo
	EMAGE:17459 same embryo
	EurExpress:euxassay_003899 same experiment
	MGI:4825833 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source