EMAGE mouse gene expression spatial database

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EMAGE:17590

Rps2 ribosomal protein S2 ( MGI:105110)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005928_01	euxassay_005928_02	euxassay_005928_03	euxassay_005928_04	euxassay_005928_05

euxassay_005928_06	euxassay_005928_07	euxassay_005928_08	euxassay_005928_09	euxassay_005928_10

euxassay_005928_11	euxassay_005928_12	euxassay_005928_13	euxassay_005928_14	euxassay_005928_15

euxassay_005928_16	euxassay_005928_17	euxassay_005928_18	euxassay_005928_19	euxassay_005928_20

euxassay_005928_21	euxassay_005928_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17590_wholemount_strong.wlz

17590_wholemount_moderate.wlz

17590_wholemount_weak.wlz

17590_wholemount_possible.wlz

17590_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17590_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T10054
Entity Detected:	Rps2, ribosomal protein S2 ( MGI:105110)
Sequence:	sense strand is shown >T10054 CTTCTTCTTCTTCTTCGGGAGAACACCAAATGGCGGATGACGCCGGTGCAGCGGGAGGGCCCGGAGGACC CGGGGGCCCAGGATTAGGTGGCCGCGGCGGCTTCCGCGGAGGATTCGGCAGCGGTCTTAGGGGCCGTGGT CGTGGCCGAGGCCGTGGCCGTGGTCGAGGCCGCGGGGCTCGTGGAGGTAAAGCTGAAGACAAGGAGTGGA TCCCCGTCACCAAGCTGGGCCGTCTGGTTAAGGACATGAAGATCAAGTCCTTGGAGGAGATCTACCTGTT CTCCCTGCCCATTAAGGAGTCTGAGATCATTGATTTCTTCCTGGGTGCGTCCCTAAAGGATGAGGTTCTG AAAATCATGCCAGTGCAGAAGCAGACTCGGGCTGGCCAGCGGACCAGGTTCAAGGCTTTCGTCGCTATTG GGGACTACAATGGTCACGTTGGTCTTGGTGTTAAGTGCTCCAAGGAGGTTGCCACTGCCATCCGAGGGGC CATCATCTTGGCCAAGCTTTCCATCGTCCCTGTGCGGAGAGGCTACTGGGGGAACAAGATTGGCAAGCCC CACACTGTTCCATGCAAGGTGACAGGCCGCTGTGGCTCTGTGCTGGTGCGTCTCATCCCTGCCCCCAGAG GCACTGGCATTGTCTCTGCTCCTGTGCCCAAGAAGCTCCTGATGATGGCCGGTATAGATGACTGCTACAC TTCAGCCAGAGGCTGCACTGCCACCCTGGGCAACTTTGCTAAGGCCACCTTTGATGCCATCTCCAAGACT TACAGCTACCTGACTCCCGACCTCTGGAAAGAGACTGTCTTCACCAAGTCTCCTTATCAGGAATTCACGG ATCATCTTGTGAAAACCCACACCAGAGTCTCTGTTCAGAGGACCCAGGCTCCAGCTGTGGCTACCACATA AGGGTTTTTATATGAGAAAAATAAAAGAATTAAGTCTGCTGACAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6749924 using vector (pDONR 201) specific primers. Forward Primer - name:T7-pDONR 201-fw, sequence:CGTAATACGACTCACTATAGGGCTCGCGTTAACGCTAGCATGGAT; Reverse Primer - name:T3-pDONR 201-rv, sequence:CAAATTAACCCTCACTAAAGGGGTGCAATGTAACATCAGAGA. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6749924 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17591 same embryo
	EMAGE:17592 same embryo
	EMAGE:17589 same embryo
	EurExpress:euxassay_005928 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source