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EMAGE:17591

Odf3b outer dense fiber of sperm tails 3B ( MGI:1917363)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17591 EMAGE:17591 EMAGE:17591 EMAGE:17591 EMAGE:17591
"Pseudo-wholemount" of euxassay_005922. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005922_01 euxassay_005922_02 euxassay_005922_03 euxassay_005922_04
EMAGE:17591 EMAGE:17591 EMAGE:17591 EMAGE:17591 EMAGE:17591
euxassay_005922_05 euxassay_005922_06 euxassay_005922_07 euxassay_005922_08 euxassay_005922_09
EMAGE:17591 EMAGE:17591 EMAGE:17591 EMAGE:17591 EMAGE:17591
euxassay_005922_10 euxassay_005922_11 euxassay_005922_12 euxassay_005922_13 euxassay_005922_14
EMAGE:17591 EMAGE:17591 EMAGE:17591 EMAGE:17591 EMAGE:17591
euxassay_005922_15 euxassay_005922_16 euxassay_005922_17 euxassay_005922_18 euxassay_005922_19
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euxassay_005922_20 euxassay_005922_21 euxassay_005922_22 euxassay_005922_23 euxassay_005922_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17591Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17591_wholemount_strong.wlz
17591_wholemount_moderate.wlz
17591_wholemount_weak.wlz
17591_wholemount_possible.wlz
17591_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17591_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T10053
Entity Detected:Odf3b, outer dense fiber of sperm tails 3B ( MGI:1917363)
Sequence:sense strand is shown

>T10053
AGTGGAGCTGGGGAAGTGTGTCTCCTAGGAATTCCTTCCACAGGTGCTGCAAGCCATAAGCGCCCCCACC
AGCGCTATGGGCTCAGAAGTCTGGGTCGGCACTTGGCGGCCACATCGGCCCCGCGGCCCCATCGCGGCGC
TCTACAGAGGCCCGGGGCCAAAATACAAGCTGCCAACGAACACCGGTTACAAACTGCACGACCCGTCTCG
GCCGCGAGCCCCCGCCTTCTCCTTCGGCTCGCGTCCACCCTTGCGGCATGCAACCTGCGGACCCGGGCCG
AGTTACCTGGTGCCTGCTCGCATGACTGTGCGCGGCACAGTTGGCTCCCCGGCCTTCTCCATCTACGGTC
GCCTAAGCCACACGGCGCCTGTCCTCACTCCGGGACCGGGCAGGTACTACCCAGAACGCGCGAGAAATGT
GACGTACCCCAGCGCTCCTCGGCATACCATCGCTCCCCGAAACTGGGGCATCCTCGCAAAGCAAGAAACC
CCAGGCCCTGGGTCCTACACCGTGCCTTCGCTCTTGGGCTCACGAGTCATAAGCAAAGTCTCAGCCCCAA
CTTACTCCATCTACAGCCGCAGCCCTGTGGGCAGCTGCTTCGAGGACCTCAGCAAGACCCCAGGCCCTTG
TGCCTACCACGTAGTGAATCCTATGATCTACAAGACTCGAGCCCCGCAGTTCACGATGCTGGGGAGGACT
CTGCCACCCCGAGAGAACACCAAGAAGCCAGGACCTGCATCCTACAGCGTGGACAAGGTGGTCTGGAGTC
GAGGGTCAAGGGGCAGGGGATGAGGGACTGAGGTCTAAGGCATGGGTCAAAGTCCCCAGGGAAATGGCAT
CCCTGTATGAAGCCGAACAGCTGGCTCGGTAAGGTCAGCCTCGTCTCCCCGACCCGCAGCACCGGAAGCC
TTGTGGCTGGAGCTTCGGGATCAGACACTCAGACTACCTAGCCCCCATGATACAGTACCACGTGGACGAC
TGATGCCTGCAAGCACGCGCGCACAGGCTCTGGGTTGTTTACTTAAAGCTTTCTAAGTCAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6748887 using vector (pDONR 201) specific primers. Forward Primer - name:T7-pDONR 201-fw, sequence:CGTAATACGACTCACTATAGGGCTCGCGTTAACGCTAGCATGGAT; Reverse Primer - name:T3-pDONR 201-rv, sequence:CAAATTAACCCTCACTAAAGGGGTGCAATGTAACATCAGAGA. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6748887 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17592 same embryo
 EMAGE:17589 same embryo
 EMAGE:17590 same embryo
 EurExpress:euxassay_005922 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS