EMAGE mouse gene expression spatial database

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EMAGE:17628

Slc30a6 solute carrier family 30 (zinc transporter), member 6 ( MGI:2386741)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005975. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005975_01	euxassay_005975_02	euxassay_005975_03	euxassay_005975_04

euxassay_005975_05	euxassay_005975_06	euxassay_005975_07	euxassay_005975_08	euxassay_005975_09

euxassay_005975_10	euxassay_005975_11	euxassay_005975_12	euxassay_005975_13	euxassay_005975_14

euxassay_005975_15	euxassay_005975_16	euxassay_005975_17	euxassay_005975_18	euxassay_005975_19

euxassay_005975_20	euxassay_005975_21	euxassay_005975_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17628_wholemount_strong.wlz

17628_wholemount_moderate.wlz

17628_wholemount_weak.wlz

17628_wholemount_possible.wlz

17628_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17628_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9527
Entity Detected:	Slc30a6, solute carrier family 30 (zinc transporter), member 6 ( MGI:2386741)
Sequence:	sense strand is shown >T9527 GCGACTTCTCAGAGCGTCTTCCGGCGGGAGCTGTACGGCTCCTTACCATGGGGACGATTCATCTCTTTCG AAAGCCACAGAGATCTTTTTTTGGAAAGTTACTACAGGAATTTAGACTTGTCGCAGCTGACCGGAGGTCC TGGAAGATCCTTCTGTTCGGTGCAATCAACGTGCTGTGCACTGGCTTCCTGCTCATGTGGTGCAGCTCCA CCAACAGCATCGCATTAACTGCCTATACTTACCTCACCATCTTTGATCTCTTTAGTTTAATAACATGTTT AATAAGCTACTGGGTAATGATGAGGAAACCTAGCCCTGTCTATTCATTTGGGTTTGAAAGATTAGAAGTC CTGGCTGTATTTGCTTCTACTGTCTTGGCACAATTGGGAGCCCTCTTTATACTAAAAGAAAGTGCAGAAC GCTTTTTGGAGCAGCCCGAGATACACACGGGAAGATTGTTAGTTGGTACTTTTGTGGCCCTTTCTTTCAA CCTGTTCACGATGCTTTCTATTCGGAATAAGCCTTTTGCTTACGTCTCTGAAGCTGCTAGTACGAGCTGG CTTCAGGAGCACGTGGCAGACCTTAGCCGCAGCTTGTGTGGCCTTATCCCAGGACTCAGCAGTATCTTCC TGCCCCGCATGAATCCGTTTGTTCTGATTGATCTTGCTGGGGCGTTTGCTCTGTGTATAACATACATGCT GATTGAGATTAATAACTACTGTGCCGTGGACACTGCGTCAGCCATTGCCATTGCCCTGATGACGTTTGGC ACCATGTACCCCATGAGTGTGTACAGTGGGAAAGTGCTCCTCCAGACAACACCACCTCATGTTATCGGTC AGTTGGACAAACTGATTAGAGAGGTGTCTACCTTGGATGGGGTGCTAGAAGTCCGAAATGAACACTTTTG GACCCTTGGATTTGGCTCATTGGCTGGGTCAGTGCATGTACGGATCCGACGAGATGCGAACGAACAAATG GTCCTTGCTCATGTGAGCAACAGGCTGTGCACGCTGGTGTCCACGCTGACTGTTCAGATCTTCAAGGACG ACTGGATTCGGCCTGCCTTATCCTCTGGGCCTGTTGCGCCCAACGTCCTAAACTTCTCAGACCATCACGT AATCCCAATGCCTTTATTAAAGAACGTGGATGAGAGGACCCCCGTCACGTCAACCCCAGCCAAGCCCAGC AGCCCACCTCCGGAGTTTTCATTTAATACGCCTGGGAAAAACGTGAGCCCGGTTATTCTTCTGAACACAC AGACAAGGCCATATAGTTTGGGTCTTAATCGCGGACACACACCATACAGCAGCGTGTTCAGCCAAGGACT CGCATTTCCAGGAGTTGGAGCAGGCCAGGGATTGAGGCCCACCTTCCCACATATACCAAGTAGATATGGA ATCAACAGGATGGGGCAGCCAAGACCCTGACAGACTCTAACTTATTGTGATGGGGAATAGCGACTTCTTG CCTTCCAATGTATTTAGTAATCCAACTTGCATTGACTGTTCAACCACTTACATGTCAGATTATTCCAGGC TTGTTCATATTTCATAAAACCTATAAACTATATTTTTGTAAAATGTTCTTGTGGCAGTGAGGTCTTTGGA AATGTCATGGGCATTAGGTAGGCCTCCTTCAAAAAGTTTGGATATGAGCTGCTTTGTAGCAGCCTATGAT GTGATAGTCTGGCCTCCAGCAGTAAAGCCATGTGCTGAGTGGATCTGCATCTTACCAAAAATTCATAATT CCTTCAGTAGAATTTATAAACATACTTCATGTATGGTGCCCAACTAAGTAAATGAAACTTAGGTGTCCAT TTGAAAAGTAAATTCTCTTTTATAGCTTTTTAAAGCTTAATTGCTACATTTTTTGAGGCCCTCCCAAATC ATTTGTTAAATAAAAAGTAACAGTTTCTATTGGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30542277 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30542277 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17632 same embryo
	EMAGE:17627 same embryo
	EMAGE:17631 same embryo
	EMAGE:17629 same embryo
	EMAGE:17630 same embryo
	EurExpress:euxassay_005975 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source