EMAGE mouse gene expression spatial database

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EMAGE:17633

Sar1b SAR1 gene homolog B (S. cerevisiae) ( MGI:1913647)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005974. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005974_01	euxassay_005974_02	euxassay_005974_03	euxassay_005974_04

euxassay_005974_05	euxassay_005974_06	euxassay_005974_07	euxassay_005974_08	euxassay_005974_09

euxassay_005974_10	euxassay_005974_11	euxassay_005974_12	euxassay_005974_13	euxassay_005974_14

euxassay_005974_15	euxassay_005974_16	euxassay_005974_17	euxassay_005974_18	euxassay_005974_19

euxassay_005974_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17633_wholemount_strong.wlz

17633_wholemount_moderate.wlz

17633_wholemount_weak.wlz

17633_wholemount_possible.wlz

17633_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17633_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9534
Entity Detected:	Sar1b, SAR1 gene homolog B (S. cerevisiae) ( MGI:1913647)
Sequence:	sense strand is shown >T9534 GAGAAGAGCTGGGGCCCTGGCAGCCATAGCGCTTTGCACAGCCGGCTTGGAGCGTGTAGGACGGGCACGG AGCAGGGTTGCGTTCTCTGAAGTCGCAGACCTGTAAGATCTGGATATGTCCTTCATATTTGACTGGATTT ACAGTGGTTTCAGCAGTGTGCTGCAGTTTCTAGGATTGTATAAAAAGTCTGGTAAACTGGTATTCCTTGG ATTGGATAATGCCGGGAAAACAACTTTGCTACACATGCTGAAAGATGACAGGCTCGGGCAGCACGTCCCA ACGCTACATCCCACTTCAGAAGAGCTTACTATTGCTGGCATGACGTTTACAACTTTTGATCTGGGTGGGC ACGTGCAAGCCCGAAGAGTATGGAAAAACTACCTTCCTGCTATCAATGGCATTGTATTTCTTGTGGATTG TGCAGACCATGAAAGGCTGTTAGAATCAAAAGAAGAACTTGATTCACTAATGACAGATGAAACCATTGCC AACGTGCCTATTCTAATTCTTGGAAATAAGATCGACAGACCTGAAGCCATCAGTGAAGAGAGGTTACGAG AGATGTTTGGTTTATATGGTCAGACAACAGGAAAGGGTAGTGTGTCACTGAAGGAGCTGAACGCCCGGCC TCTGGAAGTGTTCATGTGCAGTGTGCTAAAAAGGCAGGGCTATGGAGAAGGCTTCCGCTGGATGGCGCAG TACATCGATTAGCAGCGGCCGGCCCACACACTTCCAGCCCTCAGAGATCCATCCCCCAACATGCATAACT TGAATTCAATAGACTTTTGTTGGTTTCAGAATAGGTGTTTTTAGATTATTAATATATCTACCTGAGTGGG AATTGCAAACTGATTCAAGTTTGAATATCACAATGTTAGCTTTCTAATTCCACACAGATAGTTTTTACAG TTTCTAACCCCACATCAATCCATCACCGTTTATAAAGATCAGCTTCCCATCAGTACATTTGAAGCACTTT TTAACGATGTGAAACTACAAATATAAACCATATTTAAAAGCTCATCATGTGAAACTTTTATGTACTTTTT GGAAATAGTTTTTCAATTTTAGATTGTCTGCCTATCTTGTGTACAGTTAAAATCCGCTTATCAGTGATTG CATGATGCCTTACGGTTTCCAAGACTACTTTTTCTTATGCAAACGTCATGCAATAAAACAAACTCCAACA TTTGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30357800 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30357800 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17637 same embryo
	EMAGE:17635 same embryo
	EMAGE:17638 same embryo
	EMAGE:17634 same embryo
	EMAGE:17636 same embryo
	EurExpress:euxassay_005974 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source