EMAGE mouse gene expression spatial database

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EMAGE:17675

Galnt3 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 3 ( MGI:894695)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005997. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005997_01	euxassay_005997_02	euxassay_005997_03	euxassay_005997_04

euxassay_005997_05	euxassay_005997_06	euxassay_005997_07	euxassay_005997_08	euxassay_005997_09

euxassay_005997_10	euxassay_005997_11	euxassay_005997_12	euxassay_005997_13	euxassay_005997_14

euxassay_005997_15	euxassay_005997_16	euxassay_005997_17	euxassay_005997_18	euxassay_005997_19

euxassay_005997_20	euxassay_005997_21	euxassay_005997_22	euxassay_005997_23	euxassay_005997_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17675_wholemount_strong.wlz

17675_wholemount_moderate.wlz

17675_wholemount_weak.wlz

17675_wholemount_possible.wlz

17675_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17675_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T223
Entity Detected:	Galnt3, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 3 ( MGI:894695)
Sequence:	sense strand is shown >T223 GGAAATCTGGGGAGGTGAAAATATAGAAATGTCATTCCGAGTGTGGCAATGTGGTGGGCAGTTGGAGATT ATGCCTTGCTCTGTTGTTGGACATGTTTTTCGCAGCAAAAGCCCTCATACCTTCCCAAAAGGCACGCAGG TGATTGCTCGTAACCAAGTTCGCCTTGCAGAGGTCTGGATGGACGAATACAAGGAAATATTTTATAGGAG AAACACAGATGCAGCAAAAATCGTTAAGCAAAAATCATTTGGTGATCTTTCCAAAAGATTTGAAATAAAG AAACGCCTTCAGTGTAAAAATTTTACCTGGTACCTGAACACTATTTACCCGGAAGCGTATGTGCCAGACC TTAATCCTGTTATATCTGGATATATTAAGAGTGTGGGTCAACCTTTATGTCTGGATGTTGGTGAGAATAA CCAGGGAGGCAAACCATTGATTCTGTACACGTGCCACGGCCTCGGGGGAAATCAGTACTTCGAGTATTCT
Notes:	The probe template was PCR amplified from IMAGE:2649524 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2649524 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17672 same embryo
	EMAGE:17674 same embryo
	EMAGE:17673 same embryo
	EMAGE:17671 same embryo
	EurExpress:euxassay_005997 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source