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EMAGE:17770

Irf5 interferon regulatory factor 5 ( MGI:1350924)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17770 EMAGE:17770 EMAGE:17770 EMAGE:17770 EMAGE:17770
"Pseudo-wholemount" of euxassay_008928. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008928_01 euxassay_008928_02 euxassay_008928_03 euxassay_008928_04
EMAGE:17770 EMAGE:17770 EMAGE:17770 EMAGE:17770 EMAGE:17770
euxassay_008928_05 euxassay_008928_06 euxassay_008928_07 euxassay_008928_08 euxassay_008928_09
EMAGE:17770 EMAGE:17770 EMAGE:17770 EMAGE:17770 EMAGE:17770
euxassay_008928_10 euxassay_008928_11 euxassay_008928_12 euxassay_008928_13 euxassay_008928_14
EMAGE:17770 EMAGE:17770 EMAGE:17770 EMAGE:17770 EMAGE:17770
euxassay_008928_15 euxassay_008928_16 euxassay_008928_17 euxassay_008928_18 euxassay_008928_19
EMAGE:17770 EMAGE:17770 EMAGE:17770 EMAGE:17770 EMAGE:17770
euxassay_008928_20 euxassay_008928_21 euxassay_008928_22 euxassay_008928_23 euxassay_008928_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17770Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17770_wholemount_strong.wlz
17770_wholemount_moderate.wlz
17770_wholemount_weak.wlz
17770_wholemount_possible.wlz
17770_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17770_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35470
Entity Detected:Irf5, interferon regulatory factor 5 ( MGI:1350924)
Sequence:sense strand is shown

>T35470
CACTGATGATTACGTTCTGGGAGAAGAGGAGGAGGAGGAAGAGGAAGAGCTCCAGAGAATGCTACCAGGC
CTGAGCATCACAGAGCCTGCGCTACCTGGGCCTCCCAACGCACCCTATTCCTTACCCAAAGAAGACACCA
AGTGGCCACCTGCTCTCCAGCCACCTGTAGGGCTGGGTCCCCCTGTCCCAGACCCAAATCTCCTGGCCCC
TCCCTCTGGAAATCCTGCTGGCTTCAGGCAGCTTCTCCCTGAGGTCCTGGAGCCTGGACCTCTGGCTTCC
AGCCAGCCCCCTACAGAACCACTCTTGCCTGACCTGCTGATCAGCCCCCACATGTTGCCTTTGACGGACC
TAGAGATCAAGTTCCAGTACCGGGGACGCGCACCCCGGACCCTCACCATCAGCAACCCACAAGGCTGCAG
GCTCTTCTACAGCCAGCTAGAGGCTACCCAGGAGCAAGTGGAACTCTTTGGCCCTGTGACCCTGGAGCAA
GTGCGCTTCCCTAGCCCAGAGGACATCCCCAGTGACAAGCAGCGTTTCTATACGAACCAGCTGCTAGATG
TCCTGGACCGTGGGCTCATCCTGCAGCTGCAGGGCCAGGACCTGTACGCCATCCGTCTGTGCCAGTGTAA
GGTGTTCTGGAGTGGGCCCTGCGCCTTGGCCCATGGCTCCTGCCCCAACCCCATTCAGCGGGAAGTCAAG
ACGAAGCTCTTTAGCCTAGAGCAGTTTCTCAATGAGCTCA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 74637. Forward Primer - name:074637_F_cDNA_Irf5, sequence:CACTGATGATTACGTTCTGGGA; Reverse Primer - name:074637_N_SP6_cDNA_Irf5, sequence:TGAGCTCATTGAGAAACTGCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17769 same embryo
 EMAGE:17768 same embryo
 EMAGE:17767 same embryo
 EMAGE:17771 same embryo
 EurExpress:euxassay_008928 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS