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EMAGE:17784

Stag2 stromal antigen 2 ( MGI:1098583)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17784 EMAGE:17784 EMAGE:17784 EMAGE:17784 EMAGE:17784
"Pseudo-wholemount" of euxassay_007241. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007241_01 euxassay_007241_02 euxassay_007241_03 euxassay_007241_04
EMAGE:17784 EMAGE:17784 EMAGE:17784 EMAGE:17784 EMAGE:17784
euxassay_007241_05 euxassay_007241_06 euxassay_007241_07 euxassay_007241_08 euxassay_007241_09
EMAGE:17784 EMAGE:17784 EMAGE:17784 EMAGE:17784 EMAGE:17784
euxassay_007241_10 euxassay_007241_11 euxassay_007241_12 euxassay_007241_13 euxassay_007241_14
EMAGE:17784 EMAGE:17784 EMAGE:17784 EMAGE:17784 EMAGE:17784
euxassay_007241_15 euxassay_007241_16 euxassay_007241_17 euxassay_007241_18 euxassay_007241_19
EMAGE:17784 EMAGE:17784 EMAGE:17784 EMAGE:17784
euxassay_007241_20 euxassay_007241_21 euxassay_007241_22 euxassay_007241_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17784Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17784_wholemount_strong.wlz
17784_wholemount_moderate.wlz
17784_wholemount_weak.wlz
17784_wholemount_possible.wlz
17784_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17784_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
olfactory cortex ventricular layer
moderate moderate
homogeneousmoderate expression: see section 08 09 10 weak expression: see section 13 14 15
telencephalon ventricular layer
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 18 19 20 21 weak expression: see section 13 14 15 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6277
Entity Detected:Stag2, stromal antigen 2 ( MGI:1098583)
Sequence:sense strand is shown

>T6277
GTTTTTGAATATCTGTGGTGAGTCGTTTTCTAGTGGAAGAGGAATTTGAAAATAAAGTGTGGTAGATACA
GTGAAATTCTGTACAGATTTTTCTCTAAGGAGAATATAACATGCTTATGCTTACCAAGATGAAGTGCATT
GAGGGGCAGTTTTGTTTGCCTGAAAAAATAAAGGACAAGTAAACAATTTGATGATAAGCTACAGTTTTTC
TTAGAAAGTAAATATTTTATTTATGCGCTGTTAGTTGGCTTTTAAAGGAATTATTTCATGCTTTTTTAAA
AAATATAACAATCTGAAGAAGTATTTGGTACAGATAAGAATCTTCTCACATTTATTTACTGGTTGTACTA
AATAATGATGACCTCTGCTGGATTTCTGTTTACATCCACGAAACAAAAGTTAAGGATGGATTTATTCCCC
GACCCTGAAATTGTAGGATAGAATTGTTTTTAGCATTCTAAATTTAAATGCTTAAAACATCAATCAACAA
AACTTTGTTTTAAATGTTGTAATTGTGGAAAAAAAGTTAAACTTATAAGCAGAACTTTTACAATTTTTTC
ATCTAAAATGTATTTTAAGATATTTTTAAAATCCAAGAGCTTCTGTATACTTTTCAGAAATATCCAGATG
CAGTGAACTGCCAGAAGGTAACCAGTCTTGAACATGCTTATCCCATAGCAGCCCTGAACGTTTGCTTGTC
CTTTAAGATAAAAATGTAACGTTGTGATATTCCTTCCAGTAGTGCCACTGTATTTTGTCTCCAATAAAAG
AAGCTTATTGTAGTATTGTTTGCAGAAATTTAAACAAAATTATACAGCTTATTAGAGTGTGGGAACTATA
GTGCATC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TCAGTGGAAGAAGTTCAAGTC; Reverse Primer - name:unspecified, sequence:GAGCTTCCTCTCCACTAAGAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17782 same embryo
 EMAGE:17785 same embryo
 EMAGE:17781 same embryo
 EMAGE:17783 same embryo
 EurExpress:euxassay_007241 same experiment
 MGI:4828476 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS