EMAGE mouse gene expression spatial database

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EMAGE:18135

Dhrs3 dehydrogenase/reductase (SDR family) member 3 ( MGI:1315215)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011877. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011877_01	euxassay_011877_02	euxassay_011877_03	euxassay_011877_04

euxassay_011877_05	euxassay_011877_06	euxassay_011877_07	euxassay_011877_08	euxassay_011877_09

euxassay_011877_10	euxassay_011877_11	euxassay_011877_12	euxassay_011877_13	euxassay_011877_14

euxassay_011877_15	euxassay_011877_16	euxassay_011877_17	euxassay_011877_18	euxassay_011877_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18135_wholemount_strong.wlz

18135_wholemount_moderate.wlz

18135_wholemount_weak.wlz

18135_wholemount_possible.wlz

18135_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18135_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

sublingual gland primordium

moderate

regional

moderate expression: see section 05 06 07 12 13

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 10 moderate expression: see section 08 09 14 15 weak expression: see section 05 06 07 11 13

pons ventricular layer

moderate

regional

moderate expression: see section 08 09 11 12 13 weak expression: see section 05 06 07

midbrain ventricular layer

moderate

regional

moderate expression: see section 08 09 11 12

cornea epithelium

strong

regional

strong expression: see section 02 16 moderate expression: see section 01 weak expression: see section 17 18

bladder

moderate

regional

Expression in the fundus region.

urethra of male

moderate

regional

moderate expression: see section 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30008
Entity Detected:	Dhrs3, dehydrogenase/reductase (SDR family) member 3 ( MGI:1315215)
Sequence:	sense strand is shown >T30008 ACTTGTCGCGGGAGTCAGTCCTCATCACCGGCGGTGGGAGAGGCATCGGACGCCACCTCGCTCGGGAGTT CGCAGAGCGCGGCGCCAGAAAGATTGTTCTCTGGGGGCGGACTGAAAAATGCCTCAAGGAGACGACAGAG GAGATTCGGCAGATGGGCACAGAGTGCCACTACTTCATCTGTGACGTGGGCAACCGGGAAGAGGTGTACC AGATGGCCAAAGCTGTCCGAGAGAAGACCACCAAGGCCTTTTTGCCACGTATGCTGGAACTCCAGAACGG CCATATTGTGTGCCTCAATTCCGTGCTTGCACTGTCAGCCATCCCTGGCGCCATCGACTACTGCACGTCA AAAGCGTCAGCCTTCGCCTTCATGGAGAGCCTGACCTTGGGGCTGTTGGACTGTCCTGGTGTCAGCGCCA CCACCGTTCTGCCCTTTCACACCAGCACCGAGATGTTCCAGGGCATGAGAGTCAGGTTTCCCAACCTCTT CCCGCCACTGAAGCCAGAGACAGTAGCCCGGAGGACGGTAGATGCTGTGCAACAAAACCAGGCCCTTCTC TTGCTCCCGTGGACCATGAATATCCTCATTATCTTGAAAAGCATACTCCCACAAGCTGCACTGGAGGAGA TTCACAGGTTCTCGGGGACTTACACCTGTATGAACACCTTTAAGGGGAGGACATAGAGGCAGGAGGAAGA CACACCTGAGGAGCTATGGAGCCTGAGGGGGAGCCACAGCAGCCGGGCACACAATCCTGTGCCTGTGCAT TAGCACATCTGCTGGGTGAACGGACTGTTCTTGTCCCCAGGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3587135), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 14667. Forward Primer - name:014667_F_IRAV12-15_B21_Dhrs3, sequence:ACTTGTCGCGGGAGTCAG; Reverse Primer - name:014667_R_SP6_IRAV12-15_B21_Dhrs3, sequence:TCCCTGGGGACAAGAACA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18140 same embryo
	EMAGE:18139 same embryo
	EMAGE:18137 same embryo
	EMAGE:18138 same embryo
	EMAGE:18136 same embryo
	EurExpress:euxassay_011877 same experiment
	MGI:4824300 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source