EMAGE mouse gene expression spatial database

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EMAGE:18171

Cd44 CD44 antigen ( MGI:88338)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011897. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011897_01	euxassay_011897_02	euxassay_011897_03	euxassay_011897_04

euxassay_011897_05	euxassay_011897_06	euxassay_011897_07	euxassay_011897_08	euxassay_011897_09

euxassay_011897_10	euxassay_011897_11	euxassay_011897_12	euxassay_011897_13	euxassay_011897_14

euxassay_011897_15	euxassay_011897_16	euxassay_011897_17	euxassay_011897_18	euxassay_011897_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18171_wholemount_strong.wlz

18171_wholemount_moderate.wlz

18171_wholemount_weak.wlz

18171_wholemount_possible.wlz

18171_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18171_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

humerus

weak

regional

weak expression: see section 01 02

hip

weak

regional

weak expression: see section 06 07 11

fibula

moderate

regional

moderate expression: see section 01 weak expression: see section 03

tibia

moderate

regional

moderate expression: see section 01 weak expression: see section 03

femur

moderate

regional

moderate expression: see section 01 weak expression: see section 02 03 04 13

axial skeleton

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12

basioccipital bone

weak

regional

weak expression: see section 10 11

basisphenoid bone

weak

regional

weak expression: see section 07 08 09 10 11 12

exoccipital bone

weak

regional

weak expression: see section 01 02 04

temporal bone petrous part

weak

regional

weak expression: see section 01 02 04

orbito-sphenoid

weak

regional

weak expression: see section 01 02 05 06 07 17

viscerocranium

weak

regional

Expression in the turbinate bone.

scapula

weak

regional

weak expression: see section 01 02

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30044
Entity Detected:	Cd44, CD44 antigen ( MGI:88338)
Sequence:	sense strand is shown >T30044 ATTCCTGGGAGCTGGGACCCTCCACAGATGCAATGTGCTACTGACTATTATTATTGGGGATTATGTTTAG CATAAAATTTTCTATTCCTTTTTTATTTTATGTCATTTTTTTAGAGTCTGTTCCAATTTATGAAAATAGC ATTGCTTTCTGAAATGAGGGCCCAAATAATACTCCATTAAGAATTTGACTTTTCCAGTTCCTTCCTAGGG GCCTTGCATTACCAGGGTATGCTACCATAGGCTTCTACCAAATGCACATTCTTGGTCCCTATTGAACCTT ATCCCTACCCAAAGTCCCAGGGAACATCCACCAGCTAAGGATTTTCCCCAGAACCTAGATTGGTCTCTGG GAGGAAATTTGAATGGGTCCATATTGCCTCTCCAGCAGTCCAATCCTTAGGCATTGCTTTCCAGCGGGGT GGGAGATCAGGTGTACTGGTTACATACTCTCTTTATAGACTCCCTTCTGCTGGAAAATTTTCAGATGCTT CTGAGAGATCCCCAAAGGGTGATGCTATTTATCTTTAGTAAGCTATTTATCTTTGTTTTTGAAATATCAA ACCCTGGAGGTCCTTTGTTCAGTATGACTTTTTTTTATTTTGTTTTATTTTGTTTTGTTTTGTTTTGTTT TTTTAGGTTACTTTATCAGAAGCATAACAGGGTATAAATTGATTCATAATAAGTACCGTTCCATCTTCCA TCTTGACCCGTTGTGCTGTGATCCTTCAGTTTCTAAACAAGCAAAGTCTGAGTCTTTGTAGCACATCAGT GTGGCCTTAGTATGGTCCTCCTAAGCTCATGCTAGAGCATCTGAGCCTGCTGGGTTCACCCAGCTCAATC TCATTGAAGATCAGGGACAGGAGCACTGTTTTCATTCCAGGCTCTCAAAGGGGTTTCTCTCCTGTTCAAG AATCTGAAGTGAGAGTAGGCAAGCTTCAGTCCCTTTTGTGCTTTGGTGACCACC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3153835), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 8969. Forward Primer - name:008969_F_IRAV16-19_G13_Adra2c, sequence:ATTCCTGGGAGCTGGGAC; Reverse Primer - name:008969_R_SP6_IRAV16-19_G13_Adra2c, sequence:GGGTGGTCACCAAAGCAC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18172 same embryo
	EMAGE:18173 same embryo
	EMAGE:18169 same embryo
	EMAGE:18170 same embryo
	EurExpress:euxassay_011897 same experiment
	MGI:4823733 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source