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EMAGE:18222

Pde6d phosphodiesterase 6D, cGMP-specific, rod, delta ( MGI:1270843)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18222 EMAGE:18222 EMAGE:18222 EMAGE:18222 EMAGE:18222
euxassay_012008_01 euxassay_012008_02 euxassay_012008_03 euxassay_012008_04 euxassay_012008_05
EMAGE:18222 EMAGE:18222 EMAGE:18222 EMAGE:18222 EMAGE:18222
euxassay_012008_06 euxassay_012008_07 euxassay_012008_08 euxassay_012008_09 euxassay_012008_10
EMAGE:18222 EMAGE:18222 EMAGE:18222 EMAGE:18222 EMAGE:18222
euxassay_012008_11 euxassay_012008_12 euxassay_012008_13 euxassay_012008_14 euxassay_012008_15
EMAGE:18222 EMAGE:18222 EMAGE:18222 EMAGE:18222 EMAGE:18222
euxassay_012008_16 euxassay_012008_17 euxassay_012008_18 euxassay_012008_19 euxassay_012008_20
EMAGE:18222 EMAGE:18222 EMAGE:18222
euxassay_012008_21 euxassay_012008_22 euxassay_012008_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18222Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18222_wholemount_strong.wlz
18222_wholemount_moderate.wlz
18222_wholemount_weak.wlz
18222_wholemount_possible.wlz
18222_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18222_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30085
Entity Detected:Pde6d, phosphodiesterase 6D, cGMP-specific, rod, delta ( MGI:1270843)
Sequence:sense strand is shown

>T30085
TTCCTGGGTACCGTCTGCGAGGCCCCGCGGAGCCAGCGTGGGAGGGCCGCGGGCGGCAGGCGCCGCATCA
TGTCAGCCAAGGACGAGCGGGCCAGGGATATCCTGAGAGGCTTCAAACTAAATTGGATGAACCTTCGGGA
TGCCGAAACAGGGAAGATACTCTGGCAAGGAACAGAAGACCTGTCTGTCCCTGGTGTGGAACATGAAGCC
CGTGTGCCCAAGAAAATCCTCAAGTGCAAGGCAGTGTCTCGAGAATTGAACTTTTCTTCAGCAGAACAAA
TGGAAAAATTCCGCCTGGAACAAAAAGTTTACTTCAAAGGACAATGCCTAGAAGAGTGGTTCTTCGAGTT
TGGCTTTGTGATCCCTAACTCCACAAACACCTGGCAGTCCTTGATAGAAGCAGCGCCTGAGTCCCAGATG
ATGCCCGCCAGCGTCCTAACGGGCAATGTCATCATAGAGACAAAGTTCTTTGACGATGATCTTCTTGTCA
GCACATCCAAAGTGAGGCTTTTCTATGTTTGAGAGAATTTGTGTGCACTTCTAAGAATTTGGCCGGGGGC
AGGAGGAAGCTGAACTTGTTCTCTGCACACCTGACTGGGATACTCCAGCTGACTCACTCCTTCAACACAG
AACCCACCTGTGCACCAGGAAACCAGCTCTGAATAGACGGCCAGTGGCTTTTCTCCCAAAACCTCCATCT
TCACTGACAAGACTAAACTCCCAACCCCAGCCAAAGGCAGGGATAAGCCTCGACTCCTTCCTGGCGCAAA
CATGGGAACAAACCCTCCCAGGAGCCACGCTGCGCCTGTCACCTGCCTGCCTCCTCCTTGGGCCCCACAG
GCTTTCTT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3964336), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 15142. Forward Primer - name:015142_F_IRAV20-23_I22_Pde6d, sequence:TTCCTGGGTACCGTCTGC; Reverse Primer - name:015142_R_SP6_IRAV20-23_I22_Pde6d, sequence:AAAGAAAGCCTGTGGGGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18225 same embryo
 EMAGE:18221 same embryo
 EMAGE:18224 same embryo
 EMAGE:18223 same embryo
 EurExpress:euxassay_012008 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS