EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:18251

Fscn3 fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) ( MGI:1890386)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_012045_01	euxassay_012045_02	euxassay_012045_03	euxassay_012045_04	euxassay_012045_05

euxassay_012045_06	euxassay_012045_07	euxassay_012045_08	euxassay_012045_09	euxassay_012045_10

euxassay_012045_11	euxassay_012045_12	euxassay_012045_13	euxassay_012045_14	euxassay_012045_15

euxassay_012045_16	euxassay_012045_17	euxassay_012045_18	euxassay_012045_19	euxassay_012045_20

euxassay_012045_21	euxassay_012045_22	euxassay_012045_23	euxassay_012045_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18251_wholemount_strong.wlz

18251_wholemount_moderate.wlz

18251_wholemount_weak.wlz

18251_wholemount_possible.wlz

18251_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18251_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9788
Entity Detected:	Fscn3, fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) ( MGI:1890386)
Sequence:	sense strand is shown >T9788 GGGAACAGCTTCTGGTGGGTACTACGGGCCATATTCCCGGCCATATGGCCTGTGGGACCTTACCACTGGG GGGAAGCCTGGGCCGGACAGAGTCATCACTTGTGGCACCAGCCGCATGGCTGAGGTGGACTGGATACACA GACATCCAAAGGCGGAGGACCTAAGGGTTGGGCTCATCAGCTGGGCAGGAACCTACCTCACCTTTGAAGC ATATAAGAGTTCAGTCACAGCTTCTGCAAAGAGTTTGGGCAGGAGACAGACCTGGGAGCTCCTGGTGAGC AATGAACATGAATCACAGGCTGTGATACGACTAAAGAGCTTGCAGGGCCTCTACCTGCTGTGCGAAGCAG ATGGTACTGTGTGCTATGGCAGGCCAAGAACTAGTCACCACGGATGCTTCCTGCTCCGCTTCCACCGAAA CGGCAAATGGACCCTCCAGTGCATCATCTCTGGTCGCTATCTTGAGTCCGATGGGGAGGATGTATTCTGC AACTCCAGGGTTCTTTCAGCTTACCATATGTGGACTCCCAGGCCAGCCTTGCATGTCCACGTGATCCTCT ACAGCCCCATCTACCACAGCTATGCCCGAGCAGACCACACTGTGGGCCGAATCTGGGTAGACGCAGCAAT CCCCTGCCTAGAGGAATGTGGTTTCCTGTTACATTTCCAAGATGGATGTTACCATCTGGAGACTTCTACA CACCATTTCTTGTCTCGAGTAGACCGTCTGGTCCCTCAACGCTCATCACAGACAGCTTTTCACATGCAAG TACGGCCTCGAGGACTCGTGGCCCTATGTGATGGAGAAGGCGGCACATTGTATCCGCAGGGTTCACATCT TCTACTGGGCATGGGCTCTGCTCCCATGAAGGGCGAGGAGTGGTTTGTCCTACAGCACTTCCCGACCTGG GTCAGCCTGAAGTCCAAGTCTCGGAGGTTCCTCTCAGTCATCTATGATGCTGAGGTGTGTGCTGCTTCTG AGCGTTTGACCCAGATGTCCTTGTTCCAGTACGAATGTGACAGTGAGACTCCTACCTTGCAACTTCGTTC AGCCAATGGCTACTACCTAGCTCAGAGGAGGCATCGGGCAATCATCGCTGACGGACACCCCATGGAGTCT GACACCTTCTTCCGAGTGCACTGGAATTGTGGGAAAATCACCCTGCAGTCCCCCAATGGGCGGTTCCTGG GCATAGCCTCTGATGGCCTGCTAATGGCAAATGTCACCATCCCAGGCCCTAACGAGGAGCTGGGGATTCG ATTTGCCAATCGCCCCTTTCTCGTCTTACGCGGTCGATATGGGTATGTGGGCTCCTCCTCCGACCACGAC CTGCTGAAGTGCAACATGGATCAACCTGACTGTATTCAGCTATTGCCCTGTCGCCAGGGCATCTATCACT TTCAGGCACAGGGTGGATCCTTCTGGTCAATAACATCCTTTGGCACCTTCCGCCCTTGGGGAAAATTTGC CCTCAACTTCTGCATAGAACTACAGGGGAGCAGCTTGCTCACGGTGCTGGCACCCAATGGCTTCTACCTC CGAGCCGACCGGAGTGGCACACTATTGGCAGACAGTGAAGAAATTACCAAAGAATGTATCTGGGAATTTT AGGTCAATGGGATGTCACCTGCCAAACTGTCAATCCTCCAGGAACAACCTCTGAACTAAACAGGGCAGGA GCCCCAGAGTCACGATCCACGAGGAGGCTGTGTGCTGTGCGATTTTTCCTACCTAGTTTAGCAAAACAAC TGCTTGGAGTGACAATACATCACAAAAGGCTACCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6770861 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6770861 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18256 same embryo
	EMAGE:18257 same embryo
	EMAGE:18255 same embryo
	EMAGE:18252 same embryo
	EMAGE:18253 same embryo
	EMAGE:18254 same embryo
	EurExpress:euxassay_012045 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source