EMAGE mouse gene expression spatial database

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EMAGE:18252

Ccl27a chemokine (C-C motif) ligand 27A ( MGI:1343459)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_012042_01	euxassay_012042_02	euxassay_012042_03	euxassay_012042_04	euxassay_012042_05

euxassay_012042_06	euxassay_012042_07	euxassay_012042_08	euxassay_012042_09	euxassay_012042_10

euxassay_012042_11	euxassay_012042_12	euxassay_012042_13	euxassay_012042_14	euxassay_012042_15

euxassay_012042_16	euxassay_012042_17	euxassay_012042_18	euxassay_012042_19	euxassay_012042_20

euxassay_012042_21	euxassay_012042_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18252_wholemount_strong.wlz

18252_wholemount_moderate.wlz

18252_wholemount_weak.wlz

18252_wholemount_possible.wlz

18252_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18252_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 04 05 06 07 08 14 15 20 21 22 weak expression: see section 01 02 03 16 17 18 19

humerus

moderate

regional

moderate expression: see section 01 02 03 20 21 22

hip

moderate

regional

moderate expression: see section 11 16

fibula

moderate

regional

moderate expression: see section 03 04

tibia

moderate

regional

moderate expression: see section 02 03 04 05

femur

moderate

regional

moderate expression: see section 03 04 05 07 08 09 19 20 21 22

otic capsule

weak

regional

weak expression: see section 08 09 10 14 15

meckel's cartilage

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

axial skeleton

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13

basioccipital bone

moderate

regional

moderate expression: see section 10 11 12 13 14 15

basisphenoid bone

moderate

regional

moderate expression: see section 10 11 12 13 14

exoccipital bone

moderate

regional

moderate expression: see section 03 04 05 15 16 17 18 19

temporal bone

moderate

regional

moderate expression: see section 02 03 04 05 18 weak expression: see section 20 21

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 08 18 19 weak expression: see section 20

viscerocranium

moderate

regional

Expression in the turbinate bone.

scapula

moderate

regional

moderate expression: see section 01 02 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8379
Entity Detected:	Ccl27a, chemokine (C-C motif) ligand 27A ( MGI:1343459)
Sequence:	sense strand is shown >T8379 GCATGATGGAGGGGCTCTCCCCCGCCAGCAGCCTCCCGCTGTTACTGTTGCTTCTGAGCCCGGCTCCTGA AGCAGCCTTGCCTCTGCCCTCCAGCACTAGCTGCTGTACTCAGCTCTATAGACAGCCACTCCCAAGCAGG CTGCTGAGGAGGATTGTCCACATGGAACTGCAGGAGGCTGATGGGGACTGTCACCTCCAGGCTGTCGTGC TTCACCTGGCTCGGCGCAGTGTCTGTGTTCATCCCCAGAACCGCAGCCTGGCTCGGTGGTTAGAACGCCA AGGGAAAAGGCTCCAAGGAACTGTACCCAGTTTAAATCTGGTACTACAAAAGAAAATGTACTCACACCCC CAACAGCAAAACTAATAAAGCAACATTAGACGACAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3471454 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3471454 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18253 same assay
	EMAGE:18256 same embryo
	EMAGE:18257 same embryo
	EMAGE:18255 same embryo
	EMAGE:18251 same embryo
	EurExpress:euxassay_012042 same experiment
	MGI:4823702 same experiment
	MGI:4823701 same experiment
	EMAGE:18254 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source