EMAGE mouse gene expression spatial database

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EMAGE:18260

Tacr1 tachykinin receptor 1 ( MGI:98475)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012048. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012048_01	euxassay_012048_02	euxassay_012048_03	euxassay_012048_04

euxassay_012048_05	euxassay_012048_06	euxassay_012048_07	euxassay_012048_08	euxassay_012048_09

euxassay_012048_10	euxassay_012048_11	euxassay_012048_12	euxassay_012048_13	euxassay_012048_14

euxassay_012048_15	euxassay_012048_16	euxassay_012048_17	euxassay_012048_18	euxassay_012048_19

euxassay_012048_20	euxassay_012048_21	euxassay_012048_22	euxassay_012048_23	euxassay_012048_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18260_wholemount_strong.wlz

18260_wholemount_moderate.wlz

18260_wholemount_weak.wlz

18260_wholemount_possible.wlz

18260_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18260_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9501
Entity Detected:	Tacr1, tachykinin receptor 1 ( MGI:98475)
Sequence:	sense strand is shown >T9501 GGAATCAGTCCACCAGGGAACAGAGCTAGGTGTCTGGGGGTTTCTTTAACAGAAGGAAAGTTGACTTCCG GACTTCTGAAGCAGGGTGTGGTCACCTGTCACTTCTAGGCCACCTTGTCGCCCCCTCGCGGAGTGCTGGA TTCGTCCGGCCTGCAGAACGCCCACAGCAGGTCACGAGCAGCAGAAGGCGTGGTGGCTGCGGTGGTGGAG CGACACAGCTGCCAACAACTGGCGCAGCTGCTGCGCTGTGCTTCGGGACCAGCGAGTAGACAGTCCAGGT ACAGCCCCTTCCCCGTGACGTCTCTGCAGCGGGTTATAAAAGCCTAGTAGTGCTCACCCTGCCAAGGCTC TGCTAACTCCTGAGCACAGAAGGACCAAGACCTGGAAGCTGCGGGTGGCAGTAGAGGCTCTAAGTGAAGC AACGGAAAAAAGGAGCTCACCAGTTGGATGCGGACCTCAGAAGCCGGCCAGAGGACCAGAACTTTTACTA GCTGCGCTGCAAGCAGCCCTTCTTCCCCATCAAGCTCCCACCCAGCACCCAGCTTTGCCTTTCCTGCTGC ACGCATGTTAACGGGAGTTGCTAGTAGCAGCAGGTGCCAAAAGGCGGGTTGTCTTGCCTTTTGGAACCGT GTGGAGTGCAGCTGCTTCAATGCAAACAAGAGCCTAGCATGAAAGTGGAGGAATTTGGAGAGACCAGGCA AAGAGCTATACAGAAGCATTGCTCTTGTGGACAAGTCTCCTGCTAACCAGGACTAAGTTCTGCCCAGGAA ACTTCCCACCTCCTGAGTAGCAGGACTCAAGCCTCAGGCATCTGCGACAAGGTCCTACAGCAAGGAGGTG TGTGCGTGTGTGTGTACACTTCTGTGTGTACTTGTGAGTACCTGTAGACGCCAGGGCTTTGCTGCCTTGC CGCAAAATGGATAACGTCCTTCCTGTGGACTCTGATCTCTTCCCCAACACCTCCACCAACACTTCTGAGT CTAACCAGTTTGTGCAACCTACCTGGCAAATTGTCCTTTGGGCAGCCGCCTATACGGTCATCGTGGTGAC TTCCGTGGTGGGCAACGTAGTGGTGATATGGATCATTTTGGCCCACAAGAGAATGAGGACAGTGACCAAT TATTTCCTGGTGAACCTGGCCTTCGCTGAGGCCTGCATGGCTGCATTCAATACAGTGGTGAACTTCACCT ACGCAGTCCACAACGTGTGGTACTACGGCCTCTTTTACTGCAAGTTTCACAACTTCTTCCCCATTGCTGC TCTCTTCGCCAGTATCTACTCCATGACAGCTGTGGCCTTTGACAGATACATGGCCATCATCCACCCTCTT CAGCCCCGGCTGTCGGCCACTGCTACCAAAGTGGTCATCTTTGTCATCTGGGTCCTGGCTCTCCTGCTGG CCTTCCCACAGGGCTACTACTCCACCACAGAGACCATGCCCAGCAGAGTAGTGTGCATGATTGAGTGGCC AGAACATCCCAACAGGACTTACGAGAAAGCGTACCACATCTGTGTGACTGTGCTGATCTACTTCCTGCCT CTGCTGGTGATTGGCTATGCATACACTGTGGTAGGGATTACACTGTGGGCCAGTGAGATCCCCGGTGACT CCTCTGACCGTTACCATGAGCAAGTCTCTGCCAAGCGCAAGGTGGTCAAAATGATGATCGTGGTTGTGTG TACCTTCGCCATCTGCTGGCTGCCCTTCCACATCTTCTTCCTCCTGCCCTACATCAACCCAGATCTCTAC CTTAAGAAGTTCATCCAGCAGGTCTACCTGGCCAGCATGTGGCTGGCCATGAGTTCTACCATGTACAACC CCATCATCTACTGCTGCCTCAATGACAGGTTCCGTCTGGGCTTCAAGCATGCCTTTCGCTGCTGCCCTTT CATCAGTGCTGGTGATTATGAGGGGCTGGAAATGAAATCCACCCGATACCTCCAGACCCAGAGCAGCGTG TACAAGGTCAGCCGCCTGGAGACCACCATCTCCACTGTGGTGGGAGCCCATGAAGATGAGCCAGAGGAAG GTCCCAAGGCCACACCTTCATCCTTGGATCTCACCTCCAATGGCTCCTCTCGAAGCAACTCGAAGACCAT GACAGAAAGCTCCAGCTTCTACTCTAACATGCTGGCCTAAGCAGCAGACACATTTCCAGGGCACAGCCAC TAGCCCATCTTGTATCATTTATTCATGGAAGTCTTTGATTTGGAATTTGTTGGAAATACCCTAACACTGG AGCTTTTGGGGAAAGGTCACAATGGTTTAAGGAAAACATTCCATGCTGTATTCAATCTGGATCCCATCCT GTCTTTGCCAACCATACTCCACACTGGTGACCCAAGGCAGATTGGTGAACTTCTAAAAAATGAGAAGGCT ATCCTCGGTTTCCCCTGAAGTTTATCCCATGATTCCTGAAACGACTTCATCTGCATGCAAGGTGCTTATC TGGGGAGGGATTCTGCAAGGCAGCAGACCTATTTGCCTGGAACATCTGTCTTTCTTCAGCGAAGTCATTC TCAGCCACATGTGCAGACTAGCTCACACCACTCCAGCCTTCCACAGGACAGTGTGAGCAAAAGAAGACGC CACCTAAATCCTAAGGAAAGCTCTATCCCCACCTAAGCACACATGAATGGAGGACACTTAGCATTGTGAC ATCTTGATGTCTCAGTTCATTTAAACATCATGGGATTGACAATATTGGATTCTTCTGCATTGTGTCCCCA GTACAACCAACTCTGACTGAATATGTCTACACAAGCCAGCTGATGTTCGAACCAAGAGTCAGGAGATCCA CGCCAGGCTTAACCTATCTGAGACTCTTTTCCTTCTTTGTAAAGCAGCAGAGCTGAACCAAATACTCTAA GTGTTTCTTTGAGTGTCTGTGTTTCCTGGTTTAATGATGTATATACTTCATTCTCTCTCTCTCTCTCTCT CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTTCTCTCTCTTTTGTACACACACACACACA CACGCTCACACACTCACACACACTCACACACACACATTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT CACACACACACACACACACACACACACACACACACACACACACACACACACACACAAAATATAAAGAAAA GCAATGACAGCATCAAAAATACCAGGGCTGTCAGTCCCTAGAGATGGGTGTGTGTAAATATTCTCCCATT GATCCAAAAGCCCTTCTCCACAAATTCCTTCTCCAGGAAAGTTTTCCTCTTTGACAGAGCAGAGGACTTC TCAGGAAATCTCGGATATCTCCAGCAGGCTACAACCCAGTTCTGAGAACAGCATTGAGAGGGACAAGTGT GTGGACTTCCTGCTCTGACCAATCGAGTCTTTGTCTTCATGTTTTGCTACACATGGTGTAGAGGAGGGAG GCTGCCCTCTGCAGCTACATTCCTGGAAAGAGGAGCCTTGCCTCTTCTCTTCAGACTCTGAACCTTTCCC TCCTGCTCCACTTTGCCAACCTCTGTCTATAAGAGTTTGAAGGATCCACCAATCCAAAAGGACACTTAGT CTGCCAAGAGCCAAGAACTGAAAGTGTCACATCCTGAAACGGCTTCAGGATCCCCAGCAAGGGAGCATGC TCTTTCTTTCTGTCTCTCAGATGTGGCTGGAAGCCAAAGCTTCATGTGACATCCATGAATATCCTCATGG TTCGGGTAACTGGATGCACAAAAGCTCTATCCAGCATGTGGAGGAGAACAAGACCACTCATCAGCAGGAT TTATAACTTCTACCAGCCTTGTTGGGTCTAACCCTTTGAGGGCATCAGCATACCTGGAGAAGGCAAGCGT TATATAGAAATAATATAAGCTAGGGGCAGGGGAAGAAATGAGAAGCATACTCTGCAGAGAATTATGGGAA ATTTTAATGAACTAAGCAAAGGCCACTGAATGACAACCTATGAGTTGAAAATAGTGTAAGAACAACAAAA GCCAATGCCCAAGTTGTATATTACACTTCCAAGAAGGAATCTTGCTATATAGGGATGGAAAAAAATGTGG CTATGAAAAGTACCTAAGAAGAAAACACCCAGCATGGAAAGGCATCTCATCTACAGAAGAGATGGTTAAG GTAAAGTTGGCAAGGATTTATGGAGTGCCTGCTGTGGACTTTGCCCAAACTGAGGACACACAGCTCCTTC CACCCTGAGGAGATTCTTGTCCCTGCTATTCCTGATGATGCCAGCCTGCTTCACCAGGCATGGAGGCATT GAGAAGCAGATTCCTCCTAAGATTTTAAAAACTAACTTTCAACTTGGGGACTCCAGCCAAGGGGTGGGGC TTTCAGAGAGCTTCTAAAATGGCACGTCTCTGAGGTAACTATGAAGGGAGCCACCAGTCAGCCCTGGGAA CCTATAACTGATATGAGAAATTTCACTCCATGTGTAGTATGAGCGAAGATGAGGTGGACCGGGCTTCATG GATATGCCGAAGCATCCCTCAGAGAGGACCAGCAAGCTTCCCATTGCCCCTGCTCCAAAGACCATTTAAC TTGTAAAAATGTCGTCAATTCCTGTTTGTGAGAACACTGTGATTTGTGCTCTGTGCATGGGTCTCTTATT GAAATGTGTCACCCTGTGTTTATCTCCTGTGTTTTCTTGATGTACCCTCCAGTATCGACAGGCTGGGAGC CATCCTGGAGCCATCCTTCCTGCATTCCCTCCAACCTGTGGGATCCCAGTGCCATCGCTCTTGTTGGGCC AGGCCTCCTCCTGCCACTGTTCTTGCTTAGAGAGGAGCTCTGAAGTCTGCCCCATATTCAATGAAACTGG AGGCTGTGGAAGGACCTTGGTGGGAGGAACATCCTGAGCCCTTCAGCAGATTGCTGAGACAAAGGGCTGA GTGGAATTCTGGGAAAGGAGAGGACACAGGCCTGGAGAAAAGAATGGCCGTAGAAAGGGGTCATTCCTGA AGCATCTGTATTTGGGGCCTGGGATCCTGGAAATAAAGTATGCTTTGGCTAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30536972 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30536972 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18262 same embryo
	EMAGE:18263 same embryo
	EMAGE:18258 same embryo
	EMAGE:18259 same embryo
	EMAGE:18261 same embryo
	EurExpress:euxassay_012048 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source