EMAGE mouse gene expression spatial database

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EMAGE:18263

Fitm2 fat storage-inducing transmembrane protein 2 ( MGI:2444508)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012043. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012043_01	euxassay_012043_02	euxassay_012043_03	euxassay_012043_04

euxassay_012043_05	euxassay_012043_06	euxassay_012043_07	euxassay_012043_08	euxassay_012043_09

euxassay_012043_10	euxassay_012043_11	euxassay_012043_12	euxassay_012043_13	euxassay_012043_14

euxassay_012043_15	euxassay_012043_16	euxassay_012043_17	euxassay_012043_18	euxassay_012043_19

euxassay_012043_20	euxassay_012043_21	euxassay_012043_22	euxassay_012043_23	euxassay_012043_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18263_wholemount_strong.wlz

18263_wholemount_moderate.wlz

18263_wholemount_weak.wlz

18263_wholemount_possible.wlz

18263_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18263_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T10056
Entity Detected:	Fitm2, fat storage-inducing transmembrane protein 2 ( MGI:2444508)
Sequence:	sense strand is shown >T10056 TAGACGGTGGCCGAGAAGGCTCTGGAAGGCGGACGGTAGCCGAGAAGGACCGGCATGGAGCACCTGGAGC GCTGCGCGTGGTTTCTCCGCGGGACGCTGGTGAGGGCGACGGTGCGGCGCCACCTGCCCTGGGCGCTGGT GGCCGCCATGCTGGCGGGCTCGGTCGTCAAGGAGCTGTCCCCGCTGCCCGAGAGCTACCTCAGCAACAAG CGCAACGTCCTCAACGTGTATTTTGTTAAATTGGCCTGGGCCTGGACAGTCTGTCTCCTCCTGCCTTTCA TTGCCCTTACCAACTACCACCTGACGGGCAAGACCAGCCTGGTCCTGCGGCGGTTGAGCACCCTCCTCGT GGGCACAGCCATCTGGTATATCTGCACAGCCCTCTTCTCCAACATTGAGCACTATACAGGCAGCTGCTAC CAGTCCCCGGCCCTGGAGGGCATCAGACAGGAGCATCGCAGCAAGCAGCAGTGCCACCGGGAAGGGGGCT TCTGGCATGGCTTTGACATCTCGGGCCACTCCTTCCTCTTGACCTTCTGTGCGCTCATGATTGTGGAAGA GATGGCCGTGCTGCATGAGGTGAAGACGGACCGCGGCCACCACCTCCATGCCGCCATCACCACCCTGGTT GTCGCCCTGGGTTTCCTGACCTTCATCTGGGTGTGGATGTTTCTGTGCACAGCCGTTTATTTCCACGACT TGACCCAGAAAGTGTTCGGCACCATGTTTGGTTTGTTGGGCTGGTACGGAACATATGGGTATTGGTATCT GAAGTCTTTCTCTCCAGGACTTCCTCCCCAGAGTTGTAGTTTGACTTTGAAGCGAGATACTTACAAGAAA TGAAAGAGAAGGACATAGGGACAGGAGGACAATGGCTAATAGGATTCCCACGTACTTTGTCCAGTACAGA ATTATCTGTCCCATTTCAGAGAGGGCCCTTACTAGGTACCTTTGGTGTGGGGGGCGCTTGGGGAACAAGC TTAGTTGGCAGACTCTGCTGGCGGTGCCACCATGTTGCCAGCAGTGCTACTCCTGTCAGATTCTTCTCAT CTATGGCAAGATGTGACCTTAATCTGTCCCCAGAGGGAGAGCTGAGGCTCAGCAGAGGGAGAGCAGCCTG CCTCATCGTGTGGGTCAAGAGAGAATCAGGGCTTCAGGATTCTCTGCTGTGGCCTCCCCGGCTCTGCTTC TCTTTAGAGTTTAGAGGCCACAGTAATGCTGTTCTTCTAACAGCTTCCCCATGGCCAACTCTGAGATAAG CCTTTGTCTCTGGCAGGCTCTTTGTTAAATACCCCAATGCCTCAAGGACACTCTCTGGCTGTAGCATCCG CGTCCTCTGTGGAATGTTGACATTTCACCTGGCCATGATTCCCAACAGGGGCCCATGACCCCGAAGTGCC TGGATGGTTGTTGTTGTTGTTTCTCTGTGTAGCCCTGCGTGTTCTGGAACTCACTCTGTAGACCAGGCTG GCCTCAGATCTCTGTGAGGTTGAGGTTACCTTGCCAGGGCCACATTGTGAGCTCCTGACTGGAAGAAGAA AGAAGGAAGGAAGGAATAAAACTGAGAAGCAGACTTGTATTAGTTGATGAATGTTAAGCTGAGAGAGCAA CTCAGTGCTCACTTGTTCTGAGGCTTTCCTGCCCACTGGCTGGTGATAGAGACACAATAGGGTTTGGGGA CCTCATAGGACCACAGGACAAGTGCCACAGCCTAAGGGATTTTTACCTTTGGAGATTATGATAATATAGA AGTGAGAGCAAATGTTTCCCTCTGATGCAGGAAGCCAGCCAGCACGCAAAGCCGCCTGTATTACATACAA CCACCATGCCAAATCAGCCATATTACATAGCGCCATGACTTTGGCCCTGCCTCTTTTCTTCAGCTCACAG ACAGAACAACCCTAGGAGGGGGTGTTCAATAGCCATGAGAGTCTTTTCTTGAAGCACTTCCCGGCTTCCA GCCATGGTGTTCACCGTGACTGTTAGGGTAAGAAGTCTTGTCACCCACGTCCCATGCGGCAGGCTTCCAG TCTGATGAGAAGGGCCCTGTCCTCCCCCGGGTGATCTCCTTAAGCCTGGCACAGCTGAGCCCCTGGCCTT TTGGATGATGGTCCTTGAAGCTGCTTTGATGCCTCCTCCCTAGCAGAGCTGGCTAATGACATTACCTGAT AAGCTTTGGCCCTGACCCTGACTGGCCTGACTCAAGTTCGGGTTATTAGGCACTTAGTAAATTAAGAGAC AGCTCTTTCTTTTTCTTTTAATTTTAGTATTTTTTTTAATTTCTAGTTTAATTTAATCTTTTGAGGCAGG GTCTTACTATTCAGTCCTGGCTGGCCTGGAACTCACTATGTAGACCAGGCTGGTCTCAGACTCACAGAGA TCTACCTGCCTGCCTCGGTTTCCTAAGTGCTGGGATTAAAGGTATATATACTAAGATTAAAGCCACCACA TCTGGCTACTAAGTAATTTTTAAAATATAATAATCTATTTAAAATATGTATTTTATATCCTGTTGAACTA CAGGTCAACTTGACCAATTACTATTTAAGATGTAAGATGTTGTCACCAAAGGTGAAGAAAGGCAGGCAGA AAGGGGTCCCCATGATGTTTTGCTCTGTTGACAGTGGACTGAAAGGATATTTGACTTCCTGGGAGTGTCT ATCCATCTCCCCACGGTAGGAATGGGTCTGGGGATGATGGTAGGGCTGGTCCCCAGGTAAAGACTCTCAA GCTGCATGTTCACAACAGAAGGGGGCAGACACAGGATGTGGCCAGAGGCTCATTTGTCACACTGGGGCTC CGGGCATTTTCCTCACGTGATCTGACCAGTTTTAACATTGAGTCGACAACACGTGGGAATTCACAGTTGC TCATTGTTCTCTGCATATCAGATGGATGAAATCACACATGCTTATACTTGACACTCCTCCCTCTCTGCTC TGGGTGGGGTGGTGCCCAGGGTTCTGAGCCACACCCCAAGTTCCTCAGAGCCCTCACCTTCTCAGGAGCA TGCCTCTCCTGCCGTGTGAGGAGGCGTGGCATGGGTGAGTGTTTGGGCTTGGGCTGCCATGAGGTGCAGG TGTGAACGTTGGCATCCCTCCCAAACCACCTGACCAGCGTGCAAGCCCTTGTTGGCCTTGTCAGTAAAAT GGAGGCGATTCTTTGAGCACTGAAAGGAAATCATACATAAGATGCTGAGCATGTGTGCTCCACAGGTCAT CATCTCTCCAACTTTCTCCTGACAGTATTTAGAGCATTCTGGCTCCTTTAGAGAAAGTAAAGAAACAACT CAGAGCCTGGGTCGCCTATGGAAGTAGTCTCATGTCATCCTTCCAAGCGTCTGTTGTCCCTTGGTGGTAA ACCCCAGGCTTTGGGAGCATCTGAGCTTCCACGTGCATCCGTCACTGTCTGGGTACCAGATTGCAGTCAG AGAATAGTTCGTGCCACTGTTACCTACACACAGTCCATGTGTAAACAAGCGAATTAAAGGGCACTGGAGA AAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6750721 using vector (pDONR 201) specific primers. Forward Primer - name:T7-pDONR 201-fw, sequence:CGTAATACGACTCACTATAGGGCTCGCGTTAACGCTAGCATGGAT; Reverse Primer - name:T3-pDONR 201-rv, sequence:CAAATTAACCCTCACTAAAGGGGTGCAATGTAACATCAGAGA. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6750721 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18262 same embryo
	EMAGE:18258 same embryo
	EMAGE:18259 same embryo
	EMAGE:18260 same embryo
	EMAGE:18261 same embryo
	EurExpress:euxassay_012043 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source