EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:18265

Cdc7 cell division cycle 7 (S. cerevisiae) ( MGI:1309511)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012050. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012050_01	euxassay_012050_02	euxassay_012050_03	euxassay_012050_04

euxassay_012050_05	euxassay_012050_06	euxassay_012050_07	euxassay_012050_08	euxassay_012050_09

euxassay_012050_10	euxassay_012050_11	euxassay_012050_12	euxassay_012050_13	euxassay_012050_14

euxassay_012050_15	euxassay_012050_16	euxassay_012050_17	euxassay_012050_18	euxassay_012050_19

euxassay_012050_20	euxassay_012050_21	euxassay_012050_22	euxassay_012050_23	euxassay_012050_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18265_wholemount_strong.wlz

18265_wholemount_moderate.wlz

18265_wholemount_weak.wlz

18265_wholemount_possible.wlz

18265_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18265_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 05 06 07 19 20 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 19

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 17 18 19 20 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 09 18

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 09 18 19 20

trigeminal v nerve

strong

regional

strong expression: see section 08 09 10 17 18 19

spinal cord

strong

homogeneous

strong expression: see section 10 11 12 13 14 15 16 17

cervico-thoracic ganglion

strong

homogeneous

strong expression: see section 10 11 16

cervical ganglion

strong

homogeneous

strong expression: see section 10 17

thoracic ganglion

strong

homogeneous

strong expression: see section 12 13 14

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 15 16 17 18

neural retina

strong

regional

strong expression: see section 01 02 03 04 23 24 moderate expression: see section 22

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 15 16 17 moderate expression: see section 08 09 10 11 12 14 18

vomeronasal organ

strong

regional

strong expression: see section 15 moderate expression: see section 12

stomach

strong

regional

strong expression: see section 07 08 09 10 moderate expression: see section 04 05 06 11 12

midgut

moderate

regional

moderate expression: see section 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9565
Entity Detected:	Cdc7, cell division cycle 7 (S. cerevisiae) ( MGI:1309511)
Sequence:	sense strand is shown >T9565 CGCTCCTGAGGGAAGCGACCGCGGACGTCCTAACTTCTGTGAACCCTGCTGCTCTCGCCTGTGCACATGA CCCCGTAGCCCGCTCTTTGCATCCCAGTCGTGCGTAGTGGAAGCCTCTTTGGGGACTCAGATGGAGGAGC CAATGGCGTTTTCTTCCCTTCGTGGCAGTGACAGGTGTCCGGCTGATGACTCTTTAAAAAAATATGAGCA GAGTGTTAAACTTTCAGGTATTAAAAGAGATATTGAGGAGCTTTGTGAAGCTGTACCACAACTAGTCAAT GTGTTCAAAATTAAGGACAAAATTGGAGAAGGCACCTTCAGCTCTGTTTATTTGGCCACAGCACAGTTGC AAGAAGGACATGAAGAGAAAATTGCGCTGAAACACTTAATTCCCACAAGTCATCCTATGAGGATTGCAGC AGAGCTTCAGTGTCTGACAGTTGCAGGGGGGCAAGACAATGTCATGGGACTTAAGTACTGCTTCAGAAAA AATGATCATGTGGTTATTGCTATGCCGTATCTGGAACATGAGTCCTTTTTGGACATTTTGAATTCTCTTT CCTTCCAAGAAGTTCGGGAATATATGTATAATCTTTTTGTAGCTTTGAAACGGATTCATCAGTTTGGTAT TGTTCACCGTGATGTGAAGCCCAGCAATTTTTTATACAATAGACGTCTGAAAAAGTATGCCTTGGTGGAC TTCGGTTTGGCCCAGGGAACCCGTGACACAAAAATAGAGCTGCTCAAGTTTGTCCAGTCTGAGGCTCAGC AGGAAGATTGTTCACGAAACAAGTATCATGGAGTCGTTGGACACAAGGGCCTGCTGAGTCGCCCGGCACC TAAAACTGTGGATCAGCAGTGTACCCCAAAAACTTCTGTCAAAAGATCCTACACACAAGTTCACATTAAG CAAGGAAAAGACGGAAAGGAGAGATCTGTAGGCCTTTCTGTCCAGCGCTCTGTTTTTGGAGAGAGAAATT TCAATATACACAGCTCCATTTCACATGAGAGCCCTGCAGAGAAACTCATCAAGCAATCAAAGACTGTGGA CATAATATCACGAAAGCTAGCGACAAAAAAGACGGCCATTTCTACAAAAGCTATGAACAGCGTGATGAGG GAAACTGCCAGGTCCTGCCCTGCTGTCCTCACCTGCGACTGCTATGGATCAGATAGAGTCTGCAGCGTTT GCCTGTCGAGGCGGCAGCAGGTTGCCCCTAGGGCAGGCACACCAGGATTCAGAGCGCCAGAGGTCCTGAC AAAGTGTCCTGACCAGACCACAGCGATTGACATGTGGTCTGCAGGTGTCATATTCCTGTCCTTGCTCAGT GGGCGGTACCCATTTTACAAGGCCAGTGATGACTTAACTGCTTTGGCTCAGATCATGACAATTCGAGGAT CCAGGGAAACTATCCAGGCTGCTAAAGCTTTTGGCAAATCAGTTCTGTGTAGCAAAGAAGTCCCAGCACA AGACTTGAGAGCTCTCTGTGAGAGACTGCGGGGTCTAGACTCTACCACTCCCAGGTCAGCCAGTGGTCCT CCAGGGAATGCTTCCTATGACCCTGCTGCTTCCAAGAACACTGACCACAAAGCATCCCGTGTACAGGCTG CTCAGGCACAGCACTCAGAGGACTCCTTGTATAAAAGGGACAACGATGGCTATTGGAGTCATCCCAAAGA CTGCACTTCCAACTCAGAAGGCTGGGACTCAGTACCTGATGAAGCCTATGACCTGCTCGACAAGCTTCTG GACCTAAACCCAGCTTCAAGGATAACAGCAGAAGCAGCCTTATTACATGCGTTCTTTAAAGATATGTGCT CCTGATACGGCTCTCCACTTACTGTTGCTGCTGTGTGTTGTAAGGCGATGTGGATAATCACAGGTTGTTT GTGTCTGAGTCTAGGGTAGAATGAATAACTTGTTTTAAAGTTATAATATTTGTTCTGATGTCAGATTATA AACAGATTAAGAATTCTTAAAGAACAGGGGGAACCCTTGACAAGGTCTTTGAGTTAAACCATCAAGTAGA CTCAGATGCTTAGTGTCCTGGTTTTCTCTGCCTCAGGAGCAACCTTACTGAGCCTGATGCATGGGTTCGA GGTACAGGTCATAAAGCTAACAGGTATGACAGGGAGAAACTGAGAGAAACACTGTTTCTAAGCAGTGTGT GGTCTGCACTGTGTGCATGGGGGAACTGGCTGTTGGTGGTGTGTGTGGAAAGTGTGCAAATGAACAAATA GTTATTGCAGAGATGTTTGTCACCTCCTAACACAGTCATTGTAAGTTACATGGTGCTTACTGCAGTTTCT GGGTGCTTCACTAAGTGTTACATGTATTTATATTTGGAGCCAAATAATGTTGTGTATTATTGATAAGACA GAAATGCTTCTTTTTCTCAGTATTTTTTTCTCACATACCTTTGTTTAGTTAGGTAATGGCCACAGGAAAA GTGAGCATAGAACTGGACATTACTGGCGATGTTTTCTGGTTTGCCAGGAGCATCCACATTTCTGCATTTC TGTTCTGAATTAATCTTGGTCAGAGGGAATGGGTCTTAGGTAATTGAGAAAAAGATAAGGGGACAAAGTA GTAGAGGGCTCGATACGGAGATTTGTGCATTATTTTGTTCTAAACTTCTCTCTGTCCGAGATAGCATACC AGGCTTCCAGGAGTCAGGATGGACTTGCTGAGGAGTTCCTGCTTGTATCATCACAGACTAGGGAGCTGTC TCTCAGAGTGTTTACTGTTGTGAACATGGATAGATCAGTGTGTCTCATTGATCAATTTGCCATGTGTATA TAATGTATATTTGTGTGTATACACACTTTGTCTTATGCTGTAATTTTTTTAAAACTTGAATGTTTTGGGA TATGTATTTAATAATAAACTTTTAGATGCTTAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30611704 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30611704 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18266 same embryo
	EMAGE:18267 same embryo
	EMAGE:18264 same embryo
	EurExpress:euxassay_012050 same experiment
	MGI:4823756 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source