EMAGE mouse gene expression spatial database

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EMAGE:18267

S1pr3 sphingosine-1-phosphate receptor 3 ( MGI:1339365)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012051. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012051_01	euxassay_012051_02	euxassay_012051_03	euxassay_012051_04

euxassay_012051_05	euxassay_012051_06	euxassay_012051_07	euxassay_012051_08	euxassay_012051_09

euxassay_012051_10	euxassay_012051_11	euxassay_012051_12	euxassay_012051_13	euxassay_012051_14

euxassay_012051_15	euxassay_012051_16	euxassay_012051_17	euxassay_012051_18	euxassay_012051_19

euxassay_012051_20	euxassay_012051_21	euxassay_012051_22	euxassay_012051_23	euxassay_012051_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18267_wholemount_strong.wlz

18267_wholemount_moderate.wlz

18267_wholemount_weak.wlz

18267_wholemount_possible.wlz

18267_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18267_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9550
Entity Detected:	S1pr3, sphingosine-1-phosphate receptor 3 ( MGI:1339365)
Sequence:	sense strand is shown >T9550 CAAGTTCAGGGGCAGGCGACAAGGTCCGGGTGCTGAGCAAGGTGGCTGGACAAGCCTAGCGGGAGAGAAA CCTGAGGCCACGAGCGACCTCCGGAGGAGCCCCTAGACGGGAGTCTTAGCTGAGACACGGCAGCATGCAA CCAGCCCAGATGCGCCTTGCAGAACGAGAGCCTATTTTCAACACTCTTCCCGCAGTCGGGCCTGCACGCC TTCAGGAACCCACCACCACTCTCCCAATTGTTCCCTGAAATGAACGTTCCTGGCGCGCCCTCTCGTGGAC TGTTGAAGTAACCATCTGTCTCTAGGAAGCCATGGCAACCACGCATGCGCAGGGCCACCAGCCAGTCTTG GGAAATGACACTCTCCGGGAACATTACGATTACGTGGGGAAACTGGCAGGCAGGCTGCGGGACCCCCCTG AGGGTGGCACCCTCATCACCACCATCCTCTTCTTGGTCACCTGTAGCTTCATCGTCTTGGAGAACCTGAT GGTTTTGATTGCCATCTGGAAAAACAATAAATTTCACAACCGCATGTACTTTTTCATCGGCAACTTGGCT CTCTGCGACCTGCTGGCCGGCATAGCATACAAGGTCAATATTCTTATGTCCGGCAGGAAGACGTTCAGTC TGTCTCCAACAGTGTGGTTCCTCAGGGAGGGCAGTATGTTCGTAGCCCTGGGCGCATCCACCTGCAGCTT ACTGGCCATTGCCATTGAGCGACACCTGACCATGATCAAGATGAGGCCATATGATGCCAACAAGAAGCAC CGCGTGTTCCTTCTGATTGGGATGTGCTGGCTAATTGCCTTCTCGCTGGGTGCCCTGCCAATCCTGGGCT GGAACTGCCTGGAGAACTTTCCCGACTGCTCTACCATCTTGCCCCTCTACTCCAAGAAATACATCGCCTT CCTCATCAGTATCTTCACCGCCATTCTGGTGACCATCGTCATCTTGTATGCGCGCATCTACTGCCTGGTC AAGTCCAGCAGCCGCAGGGTGGCCAACCACAACTCTGAGAGATCCATGGCCCTTCTGCGGACCGTAGTGA TTGTGGTGAGTGTGTTCATTGCCTGTTGGTCCCCACTTTTTATCCTCTTCCTCATCGACGTGGCCTGCAG GGCAAAGGAGTGCTCCATCCTCTTCAAGAGTCAGTGGTTCATCATGCTGGCTGTCCTCAACTCGGCCATG AACCCTGTCATCTACACGCTGGCCAGCAAAGAGATGAGGCGCGCCTTCTTCCGGTTGGTGTGCGGCTGTC TAGTCAAGGGCAAGGGGACCCAGGCCTCACCCATGCAGCCTGCCCTCGACCCAAGCAGAAGTAAGTCAAG CTCTAGTAACAACAGCAGCCACTCTCCGAAGGTCAAGGAAGACCTGCCCCGCGTGGCTACTTCTTCCTGC ATCATTGACAAAAACAGGTCGTTTCAGAACGGGGTCCTCTGCAAGTGACGGTCTCAACAGGGCAAGCTCT GCAGCCACACCTATTTATTGCATGCATCACTTCCACGTGGGGCCTTAAGAGATCTTAACTCTGGAGGTCT GCTTGCTGTGGCCAGACTGTGTGGTATCTCCTGAGGAGGAGACCCAAGGAGTCACCAACGCATTTCAGCC TGGACTTGGATGTGACTTATCTGTTGCGGGCTCCATCCTTCTGAATGTACCAAGCTGCTGACACGGTGCA CTGCCTTCGGATGCCGCTACCCCTGGTCCAGGGAAGGCAGGTGGTGATGTGCTATGCACATGCACATTGG GATGATGTCTCCCTGCGTTCACACTACATTTATTTTCCATAGAATGGGTGCTCATGTGTGTGTGTGTGTG TGTGTGTGTTCGTTGCTGTGTTGTACAGGATGTATACGATTGATTTTCCCAGGTGGAACCCATTACAAAG TGTCAGTCTTCCACACATCATTGGTCCAAACCTGCCCACACTGCTGTCCCAGGTTCAGCATGTGATAAGG TCATGTCCCCTTCCCAGTGCCCTTGCACTGGTGTTCTAACTCATAGCTGTGAAAAGTTACTAAAGTATTC TGTATTCTCAAAAGCCTCACCCAAGAGCAGCCCAGGTGTGTGGGTCTTACCCTTACATTCCTGCACCCCT CCTCCCTCTTGGGTCCCTTGCGTAAGCTTAGGTACACCTAAGAGCCAAATTATGCGATCATTGAGGCATT TCTGAAAGCCACAGAAAAGCCAGCCGGGATCTCTCGGAATGCAGGCACCACTAAAGGAAGAACTCCTAAG GAGAGGCAAGGTGCTCTCCACTGTGGGCAATCTTTCTAGGGGACTGCAGTCTGTCTGCTGCATGTAGAGA GTTTCCAGCAGTGACGTTCACAAGCCAGCCCTTCATCTGTATGGTCCTGAGTTCAAGTCTATAGAGAGAA GCTTGAGGCTGCTTGTCCCCTTTGCTGTCACCCTTTGCTGGCAAGGAGCTGGAGCTGTCAGCAAAGCTTC TCAACTCCCTCAGTTATGGACCCTGTCCAGGACTATTCAGTGAGAGGATTCCGCCAGGCTGGGAGGCGTG ATGTAGTTATTTGTGTGCTCTTTAGAGAGAACCATTCCAATTCCCATATGATGGACACTCCAGCCACTCT CCTCCGCTTGGACCTGCGTTCATTTGTCCATCCTGTAAAGAAAATGCGTAGAAGACACCTCTGAACATCC CCTCCCCAAGGATTCCGCCCACAGGGCACACAGATGAAAGGTGGGTGCGTTGGAGAGTGGCCAGCATCCA TGCTCCCAGAACAGCCTTCGGAAAGACTCTCATTTGAGGAAACGTCATGTCAAGGCTCCCCATAAGTTGA TTAGTCTCAGAGACTGTTGCCTTATGAACCCTGGAAGAGCAGCCTTTACACACTCATGGGCCTTTTTAAA ACTGATTTTTTTTTCCCTCTTGGTGGTTACCTGATGGCATTTGCTCTTGTTTAGGGACATTTTTCTGGGC CTTAGGTAACCCTGGTCTGACTAACTAAAGACTTCCCCTCATGTCACTCCTGGTGTATATAGGTCACTGC CAAGAAGTTCTGCTCCATCATTGGCCTTGCCCTTCTCTATGTCACCAAGAAAATGTCACCGTGTTGACAA AACCCATGGCATTTCTGGAAAAGGAAAGCATACAAGTGCTGGGTTAAGGGAAAAATAAATAGCAGAGCAG TGTGTAGGGGAGTTGAGGTGGAAAGAGAACAGGGCTTGTGGGCACGCTCTTTCATGATTGCAAGGCTTTG AGGCCGTGGGTCTAGGTCACTGATATTAATCACACATTGTACAGGATTCTGGCTTCTCCATTTCCTCCTG TGTCTGCTTTCACACAACAGCCCTTGTGGAGGGCCAGGCCCACCATCCCCAGCTAGGAGGTGACATTTGT AGCATGACAGACTGCCAAGCAACTGAAAATAAAAATACACTAAAACCCAATTGCGCTCACTTGATGTATG CCCAGTGGATCCGTCGACCGTGATGCCAGTGTGCACACCTTTCCCTAGCTAGCCTCCGAGACAGCACTGA GACATCGGGTGCATCCAACCCTCACCCTGAAGGTCAAAGGTCGGAAACCAGTGACACCAGAGACCCTGGG GCTCGCCCAAGGTAGTGAGCAACCTTGGCTACTTGCAGTTTATGTTTGTTAATAATAAATAAAACCAAAA GCCTGTATAAAAACCCAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30613259 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30613259 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18266 same embryo
	EMAGE:18265 same embryo
	EMAGE:18264 same embryo
	EurExpress:euxassay_012051 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source