EMAGE mouse gene expression spatial database

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EMAGE:18293

Cd248 CD248 antigen, endosialin ( MGI:1917695)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012090. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012090_01	euxassay_012090_02	euxassay_012090_03	euxassay_012090_04

euxassay_012090_05	euxassay_012090_06	euxassay_012090_07	euxassay_012090_08	euxassay_012090_09

euxassay_012090_10	euxassay_012090_11	euxassay_012090_12	euxassay_012090_13	euxassay_012090_14

euxassay_012090_15	euxassay_012090_16	euxassay_012090_17	euxassay_012090_18	euxassay_012090_19

euxassay_012090_20	euxassay_012090_21	euxassay_012090_22	euxassay_012090_23	euxassay_012090_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18293_wholemount_strong.wlz

18293_wholemount_moderate.wlz

18293_wholemount_weak.wlz

18293_wholemount_possible.wlz

18293_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18293_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 09 21 22

radius

strong

regional

strong expression: see section 01

humerus

strong

regional

strong expression: see section 01 02 03 04 05 moderate expression: see section 22 23 24 weak expression: see section 06

tarsus

moderate

regional

moderate expression: see section 04

fibula

moderate

regional

moderate expression: see section 02

tibia

moderate

regional

moderate expression: see section 01 02 03

femur

strong

regional

strong expression: see section 06 moderate expression: see section 03 04 05 07 19 20 21 22 23 24

otic capsule

moderate

regional

moderate expression: see section 09 10 weak expression: see section 07 08 17

meckel's cartilage

strong

regional

strong expression: see section 07 08 09 10 22 moderate expression: see section 04 05 06 11 15 16 17 18 20 21

axial skeleton

moderate

regional

moderate expression: see section 09 10 11 12 13 14 18 19 weak expression: see section 15 16 17

basioccipital bone

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

basisphenoid bone

moderate

regional

moderate expression: see section 12 13 14 15

exoccipital bone

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 19 21 22 23

temporal bone

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 21 22 23 24

orbito-sphenoid

moderate

regional

moderate expression: see section 02 05 06 07 19 24 weak expression: see section 18

viscerocranium

moderate

regional

Expression in the turbinate bone.

clavicle

moderate

regional

moderate expression: see section 07 21

scapula

strong

regional

strong expression: see section 05 moderate expression: see section 23 weak expression: see section 04 22 24

pelvic girdle skeleton

strong

regional

strong expression: see section 10 moderate expression: see section 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7791
Entity Detected:	Cd248, CD248 antigen, endosialin ( MGI:1917695)
Sequence:	sense strand is shown >T7791 GTCAAGAGCAGCGGCAGGCCCGAGCCGGGCCAGTCGGGGGGCGTCGCGATGCTGCTGCGCCTGCTGCTGG CCTGGGTGGCCGCGGTGCCCGCACTGGGCCAGGTCCCCTGGACGCCGGAGCCTCGAGCCGCGTGCGGCCC CAGCAGCTGCTACGCGCTCTTTCCCCGGCGCCGCACATTCCTGGAAGCTTGGCGGGCGTGCCGCGAATTG GGGGGCAACCTGGCCACACCGCGGACCCCAGAGGAGGCCCAGCGTGTGGACAGCCTGGTGGGGGTCGGGC CGGCCAACGGGCTGCTATGGATTGGGTTGCAGCGGCAGGCTAGGCAATGCCAGCCGCAGCGCCCACTGCG GGGCTTCATATGGACCACGGGAGACCAGGACACCGCCTTCACCAACTGGGCCCAGCCGGCTACGGAAGGA CCCTGCCCAGCCCAGCGCTGTGCAGCCCTTGAGGCCAGCGGAGAGCATCGCTGGCTCGAAGGCTCGTGCA CACTGGCTGTCGATGGCTACCTCTGCCAGTTTGGTTTTGAGGGTGCCTGCCCTGCCTTGCCGCTTGAGGT GGGTCAGGCCGGTCCCGCTGTCTACACCACACCCTTCAACCTGGTTTCCAGCGAGTTCGAATGGCTGCCC TTTGGCTCCGTGGCAGCTGTGCAGTGCCAAGCTGGCAGGGGAGCTTCTCTGCTGTGCGTGAAACAGCCTT CAGGTGGCGTGGGCTGGTCCCAGACTGGCCCGCTGTGCCCAGGGACTGGCTGTGGTCCTGACAATGGGGG TTGCGAACATGAGTGTGTGGAAGAGGTGGACGGTGCTGTGTCCTGCCGCTGCAGTGAAGGCTTCCGTCTA GCAGCAGATGGGCACAGTTGTGAAGACCCCTGTGCCCAGGCCCCCTGTGAGCAGCAGTGTGAACCTGGAG GGCCACAAGGCTATAGCTGCCACTGTCGCCTTGGCTTCCGGCCAGCTGAGGATGATCCACACCGCTGCGT GGACACGGATGAGTGCCAGATTGCTGGTGTGTGCCAGCAGATGTGTGTCAACTATGTTGGTGGCTTTGAG TGTTACTGCAGCGAGGGTCACGAGCTTGAGGCAGATGGTATCAGCTGTAGCCCTGCAGGAGCCATGGGTG CCCAGGCTTCCCAGGATCTCAGAGATGAGTTGCTGGATGATGGAGAAGAAGGGGAGGATGAAGAGGAGCC CTGGGAGGACTTTGATGGCACCTGGACAGAGGAACAGGGGATCCTATGGCTGGCACCTACACATCCACCT GACTTTGGCCTGCCCTATAGGCCCAACTTCCCACAGGATGGAGAGCCTCAGAGATTGCACCTGGAGCCTA CCTGGCCACCCCCACTTAGTGCCCCCAGGGGCCCCTACCACTCCTCAGTGGTGTCTGCCACACGGCCCAT GGTGATCTCTGCCACTCGACCCACACTACCTTCTGCCCACAAGACCTCTGTTATTTCAGCTACACGCCCA CCCCTGAGCCCTGTCCACCCACCTGCCATGGCCCCTGCCACACCTCCAGCTGTGTTCTCTGAGCACCAGA TCCCCAAAATCAAGGCCAATTATCCAGACCTGCCTTTTGGCCACAAGCCTGGGATAACCTCGGCCACTCA CCCAGCACGGTCTCCTCCGTACCAGCCCCCCATTATCTCAACCAACTATCCCCAAGTCTTCCCTCCCCAC CAGGCCCCTATGTCTCCAGATACCCACACTATCACTTATTTGCCTCCAGTCCCCCCTCACCTTGATCCTG GGGATACCACTTCTAAAGCCCATCAACACCCTTTGCTCCCAGATGCTCCAGGTATCAGAACCCAGGCCCC CCAGCTTTCTGTCTCAGCTCTCCAGCCCCCTCTTCCTACCAACTCCAGGTCTTCTGTCCATGAAACTCCT GTGCCTGCTGCCAACCAGCCCCCAGCCTTCCCTTCTTCTCCCCTCCCCCCTCAGAGGCCCACTAACCAGA CCTCATCTATCAGCCCTACACATTCCTATTCCAGAGCCCCTCTAGTCCCAAGGGAAGGAGTTCCCAGTCC CAAATCAGTGCCACAGCTGCCCTCGGTGCCCTCCACAGCAGCTCCAACAGCCCTGGCAGAGTCAGGTCTT GCAGGCCAAAGCCAAAGGGATGACCGCTGGCTGCTGGTGGCACTCCTGGTGCCAACATGTGTCTTCTTGG TGGTGCTGCTTGCCCTGGGCATTGTGTACTGCACTCGCTGTGGCTCCCACGCACCCAACAAGCGGATCAC GGACTGCTATCGCTGGGTCACACATGCTGGGAACAAGAGCTCAACAGAACCCATGCCCCCCAGAGGCAGC CTTACAGGGGTACAGACCTGTAGAACCAGTGTGTGATGGGGTGCAGATGCCCCTTTGTGGGATAGAAGAA AAGGACTTGCTTTGGACACATGGCTGAGACCACACCAAGGACTTATGGGGGCTGCCCAGCTGACAGAGGA GGTTCTGTTCTTTGAGCCCAGCATCCATGGCAAAGGACACACCAGGACTCCAGGACCTCAAGGGGTGGGT GCTGGGATCTTCTCCAATAAATGGGGTGCCAACCTCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6314798 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6314798 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18296 same embryo
	EMAGE:18298 same embryo
	EMAGE:18297 same embryo
	EMAGE:18294 same embryo
	EMAGE:18295 same embryo
	EurExpress:euxassay_012090 same experiment
	MGI:4823724 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source