EMAGE mouse gene expression spatial database

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EMAGE:18295

Trappc5 trafficking protein particle complex 5 ( MGI:1913932)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18295_wholemount_strong.wlz

18295_wholemount_moderate.wlz

18295_wholemount_weak.wlz

18295_wholemount_possible.wlz

18295_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18295_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7776
Entity Detected:	Trappc5, trafficking protein particle complex 5 ( MGI:1913932)
Sequence:	sense strand is shown >T7776 GTTGAGACCTTTCCCCGCCCGAGAACCTTCTCCGGCGCGCTCGGGCCTCGTCTTTTTTTCTGTGCGTCCG GAGCAGCGTTCCCGGGACTGCATCATGGAGGCGCGTTTCACACGCGGGAAGTCGGCGCTGCTAGAGCGCG CACTGGTGCGGCCCCGCACCGAGGTGAGCCTGAGCGCCTTTGCCCTTCTCTTCTCCGAGCTGGTGCAGCA CTGTCAGAGCCGCGTTTTCTCTGTGGCCGAGCTTCAAGCACGCTTGGCTGCCCTGGGCCGACAGGTGGGC GCCCGAGTCCTAGATGCCCTGGTGGCCCGTGAGAAGGGTGCCCGGCGTGAGACTAAGGTGCTGGGCGCAT TGCTCTTCGTCAAGGGTGCGGTGTGGAAGGCACTGTTCGGCAAGGAGGCGGACAAGCTGGAGCAGGCCAA TGATGACGCCCGCACTTTCTACATCATCGAGAGGGAGCCGCTCATTAACACCTACATCTCCGTGCCCAAG GAGAACAGCACACTGAACTGTGCCAGCTTCACAGCGGGCATCGTGGAGGCTGTGCTCACGCACAGCGGCT TCCCTGCCAAGGTTACGGCGCACTGGCACAAGGGGACCACACTCATGATCAAATTTGAAGAGGCTGTGAT TGCCCGAGATCGGGCCCTGGAGGGACGCTGACCCCACAGGAGATAAAGGACGAGCAGATCCTGTTCCCAA GCTTGTGTTGTGTCTTGTGTAGGGGCGTCAGATCTGCCCAGATCCTGATCCTGGTCCTGAGTCCCAGACT AAGGGAGTCTGAAGGCAAGCTTGTCAGGCATAGATGGAGAGTAAGGAAGTGGGCAATGGCCGACTTGGCT TCTGGCTTTCAGTTAAGGTTGGAGGCTTGGATGTATCGGGGAGATAGGGCACTTACAGAATTATTGTTGG AACCTGCAGTCTGGAGAGGTGAGGGCGGGGATATGAGGTTAAGAGGGACCGGGACCTGGCAGACTGCCAG TGCTATAGCCCAGTTTGTAGAGTGCCGTGGGTTTGATTCCTTATGCCACATAAAGTGGGTACAAATTGTC TTATCCCAGCACTAGGAAGGTAGAGAGAGAAAGATGGTAAGTTCAAGGCCATCCTCTGCTACTTAGCAAG TTTGAGACCAGCTCTGGCAGCACAGCCTCTTAAGTGTCAGGCAGGTGTACTCTTCCTTTGAAACTGTTTT CTTCAAGTCTCCATTTGTTCTAAAAAACAAAAAACCCAAAAGCCAGGTAGTAAAGGGGAGGGTGTGGCTA TGTGCCTAAAATAAATGTAGAGTGGTTATTCGCCCTTTAGACATCGGCTACTCAAAGTGGCTTGTGGACT GTCAGCATCAGTATCAGCATTATCACTACCAGCAGCAACACCAACGTCACCATCCTCGTCCCACATGCAG GATCACTGGTCCACCCCTATACCTCCTGGACCAGAAGTGTGTATTCCAGCCCCTTTCTCAGAGGTCTCCC TGGGACTCTTATTTCAGGGCATCAGAGTCACCCGAGGGACCAAGAAACTCAGCTGGTTTCAGAACAGAAC AGAACTGTAAGTTTCACCCCTACAGTCAGATTCTGTAGGTTTGGGGTGTGCATAAGAATTTGTTTTTGGT ACATTCTGGAGAGCTGCTGGGTGCCCCAGGGATCACACCGAGACCAGTGGCACACCAGCCTCTCAAAGGG GGAAGAGCCCAGAACCATCTGGAAACTTGAGGGTTTTAAGGTTAATATTTACAAAGTTTGTTCTGAGACA GGGTTCCGGCCGCCCAGGCTGGCTTCAGCCTTGCTGTATATTTAAGGGTGACTGTGAATCCCAACCCTTC TGACTCTGCATGAGGTTGAGTGTTTAACTTAGTGGCTTATCACATTTACTATTTTACGCAGAGTCTGAAT TATGTTTTCCTGGCTGACCTGGAGCGCACTATGTAGACCAGGCTGGCCCTATACTCAAGAGGTCTACCTC CCTAGTGCTGAGATTAAACTTGAGCACCACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6313540 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6313540 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18296 same embryo
	EMAGE:18298 same embryo
	EMAGE:18297 same embryo
	EMAGE:18294 same embryo
	EMAGE:18293 same embryo
	EurExpress:euxassay_012093 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source