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EMAGE:18340

Slc25a30 solute carrier family 25, member 30 ( MGI:1914804)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18340
euxassay_014289_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18340Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18340_wholemount_moderate.wlz
18340_wholemount_weak.wlz
18340_wholemount_possible.wlz
18340_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18340_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31450
Entity Detected:Slc25a30, solute carrier family 25, member 30 ( MGI:1914804)
Sequence:sense strand is shown

>T31450
CCATCACAGCAGAATGCGGCACATTTCCAATTGATTTAACTAAGACACGGCTTCAGATTCAAGGCCAGAC
AAATGATGCCAACTTCCGAGAGATCAGGTACCGAGGCATGCTGCACGCGCTGATGAGGATAGGCCGAGAA
GAAGGCCTGAAGGCGCTGTATTCAGGGATTGCACCCGCAATGCTGCGCCAGGCCTCCTATGGAACCATCA
AGATCGGCACGTACCAGAGCCTGAAGCGGTTAGCTGTGGAACGCCCAGAAGATGAAACCCTGCTGGTCAA
TGTTGTGTGTGGAATTCTGTCTGGAGTCATATCCTCAGCTATTGCTAATCCGACTGATGTTTTGAAAATC
CGAATGCAAGCACAGAACAGCGCTGTTCAAGGAGGCATGATAGACAGCTTCATGAGCATTTACCAGCAGG
AGGGGACTAGAGGACTGTGGAAGGGTGTGTCCCTCACAGCCCAGAGGGCAGCCATTGTGGTCGGCGTGGA
GCTTCCAGTCTATGACATCACCAAGAAGCACCTGATACTCTCAGGCCTGATGGGAGACACTGTCGCAACG
CATTTTCTGTATGTTTATGGATTTTAAAATTAATATTTTAGGTATGCATGAGCTTGGTAGGGTGAAAATT
TTACATAAGTTTATGGAAGTTTTAGAGAAAGTATTGTAATAGTGTGGTATTTTCTTATTTGAAAAATTAT
AACTCAGAAGTCGAATGGGATGAGGTGAAGTCTTTGCCTTTACACAGGTAATTATATACCTGTATGTGTA
CAAACTGTATGTTTGTAAGCACCATGGACTTTATAAACAAATAAGGTCATGTTTGCATTGTATTACTTTT
TAAAAAGTGACAACACAACCCAAATACATTTTCACTTCAGCTCTTACACCTTAGTCATTTTTGATTACTG
TTTGTGTATGTGCCTGCAGAGGTCAGAGGGGTGTTAGAGCCCAGAGCCAGAGCTCCAGGCACTTGTGAGC
C
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4238839), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 26568. Forward Primer - name:026568_F_IRAV59-62_J11_4933433D23Rik, sequence:CCATCACAGCAGAATGCG; Reverse Primer - name:026568_R_SP6_IRAV59-62_J11_4933433D23Rik, sequence:AGGCTCACAAGTGCCTGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18338 same embryo
 EMAGE:18342 same embryo
 EMAGE:18337 same embryo
 EMAGE:18341 same embryo
 EMAGE:18339 same embryo
 EurExpress:euxassay_014289 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS