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EMAGE:18355

Mark3 MAP/microtubule affinity-regulating kinase 3 ( MGI:1341865)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18355 EMAGE:18355 EMAGE:18355 EMAGE:18355 EMAGE:18355
"Pseudo-wholemount" of euxassay_014296. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014296_01 euxassay_014296_02 euxassay_014296_03 euxassay_014296_04
EMAGE:18355 EMAGE:18355 EMAGE:18355 EMAGE:18355 EMAGE:18355
euxassay_014296_05 euxassay_014296_06 euxassay_014296_07 euxassay_014296_08 euxassay_014296_09
EMAGE:18355 EMAGE:18355 EMAGE:18355 EMAGE:18355 EMAGE:18355
euxassay_014296_10 euxassay_014296_11 euxassay_014296_12 euxassay_014296_13 euxassay_014296_14
EMAGE:18355 EMAGE:18355 EMAGE:18355 EMAGE:18355 EMAGE:18355
euxassay_014296_15 euxassay_014296_16 euxassay_014296_17 euxassay_014296_18 euxassay_014296_19
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euxassay_014296_20 euxassay_014296_21 euxassay_014296_22 euxassay_014296_23 euxassay_014296_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18355Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18355_wholemount_strong.wlz
18355_wholemount_moderate.wlz
18355_wholemount_weak.wlz
18355_wholemount_possible.wlz
18355_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18355_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31475
Entity Detected:Mark3, MAP/microtubule affinity-regulating kinase 3 ( MGI:1341865)
Sequence:sense strand is shown

>T31475
CAGGCCATGAGGAGGATGAGCTTAAGCCATTTGTTGAACCAGAACTAGACATCTCAGACCAGAAGAGAAT
AGATATTATGGTGGGAATGGGATATTCCCAAGAAGAAATTCAAGAATCTCTTAGCAAAATGAAATACGAT
GAAATCACAGCTACTTACTTGCTACTGGGGAGGAAGTCTGCAGAGCTGGACGCTAGTGACTCCAGTTCTA
GCAGCAACCTTTCACTTGCTAAGGTGCGGCCAAACAGTGACCTCAGCAATAGCACTGGCCAGTCTCCTCA
TCACAAAGGGCAGAGAAGTGTTTCCTCAAGTCAGAAGCAGAGACGGTACAGTGACCACGCTGGACCAGCT
ATTCCTTCAGTTGTGGCATATCCAAAGAGGAGTCAGACCAGCACTGCAGATAGTGACCTCAAAGAAGATG
GAATTCCCTCCCGAAAATCAAGCAGCAGTGCTGTAGGAGGGAAGGGGATTGCTCCTGCCAGTCCCATGCT
TGGGAATGCAGGCAATCCCAATAAAGCCGATATCCCTGAGCGCAAGAAAAGCCCTGCTGTGCCCAGTAGT
AACACAGCATCTGGTGGGATGACACGGCGGAACACTTACGTTTGCAGCGAGAGATGTGCAGCAGACAGAC
ATTCTGTGATTCAGAACGGCAAAGAAAACAGCGCTATTCCAGATGAGAGAACTCCAGTTGCTTCAACCCA
CAGTATTAGCAGTGCGACCACCCCAGATCGCATCCGCTTTCCAAGGGGCACAGCCAGCCGTAGCACTTTC
CATGGCCAACCTCGGGAGCGGCGAACCGCAACATATAATGGCCCACCTGCCTCACCCAGCTTGTCCCATG
AAGCCACACCGCTGTCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4459439), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 26643. Forward Primer - name:026643_F_IRAV59-62_P16_Mark3, sequence:CAGGCCATGAGGAGGATG; Reverse Primer - name:026643_R_SP6_IRAV59-62_P16_Mark3, sequence:GAGACAGCGGTGTGGCTT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18354 same embryo
 EMAGE:18356 same embryo
 EMAGE:18353 same embryo
 EMAGE:18352 same embryo
 EurExpress:euxassay_014296 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS