EMAGE mouse gene expression spatial database

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EMAGE:18385

Actr1b ARP1 actin-related protein 1 homolog B, centractin beta (yeast) ( MGI:1917446)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014307. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014307_01	euxassay_014307_02	euxassay_014307_03	euxassay_014307_04

euxassay_014307_05	euxassay_014307_06	euxassay_014307_07	euxassay_014307_08	euxassay_014307_09

euxassay_014307_10	euxassay_014307_11	euxassay_014307_12	euxassay_014307_13	euxassay_014307_14

euxassay_014307_15	euxassay_014307_16	euxassay_014307_17	euxassay_014307_18	euxassay_014307_19

euxassay_014307_20	euxassay_014307_21	euxassay_014307_22	euxassay_014307_23	euxassay_014307_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18385_wholemount_strong.wlz

18385_wholemount_moderate.wlz

18385_wholemount_weak.wlz

18385_wholemount_possible.wlz

18385_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18385_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 13 15 16 17 18 19

vomeronasal organ

strong

regional

strong expression: see section 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31480
Entity Detected:	Actr1b, ARP1 actin-related protein 1 homolog B, centractin beta (yeast) ( MGI:1917446)
Sequence:	sense strand is shown >T31480 TTGCCCCGAAGGATGTTAAAATCAAGATCTCTGCTCCTCAGGAACGGCTGTACTCCACGTGGATCGGAGG CTCCATCCTCGCCTCACTGGATACCTTCAAGAAGATGTGGGTATCCAAAAAGGAATATGAAGAAGATGGC TCCCGCGCGATTCATCGCAAAACCTTCTAATGCCCGAGGAGGGTGGACATACTGGGAGAGAGGGAGAAAA GGGGAGCCAGAGCCCTTGACCCCTTTTGGTCTGGGTTCGTGTTCTAAGATTAGGGTCCCCTGCATGCCCT GACCCCGGAGCAGGTGGTGCGATCATGGTCACCTGTAGCCCTCCCCTCCCCTCCCCCTGTGCTCAACACA CACACAGACACACACAGAGTAATGATTGGCTGCATGGCTGGCAGTCCCAAGCTGGAGCATCGGAACTGAG GAACCCAGCATCGAGCTGGTGTGAGTGACCCTCTTGCAGTGCCACTGTGGCCTGTGGTTCCTTGCTCTGG CCTTGAAGCTCTGAGACCAGACTCACATGCTCCATTTGCTCTGACAGGCTCCCAATGGGGCTAGACTCTC TGGATCTCTGGAGTAGGCTGAAGGGAGGGACAGCCAGGAGCTCCCCTCAGGGTGGTGAGGCACAGTGGTG TTTGATGCTGCCTCCTCTTTCTGGCCTGGCAAGGCAAATGTCCTGCATAGGACTGACTAGGGGACAGTCC TCCATCCACTGAGGGAATGCAGCCAAGAGTCAGAGCATCAGAGCCATGGGCAGCCACAGCTCGTGGTTTG CCCCACTCTCTTTCAAAGCACTACACTGATTTGCTACTCCCTCCTCGTGCCCTCCGTGCCCTGGACAGGA AGGGGTTAATGGTCTTTATTTGCTAAAGGAAAGCCATTTAATCAGGATTCAGACCCAAAGGGAAGGCATG TTCTTACCTCTATCAAGAAAGGTCTTCACCATGACCAGGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5375425), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 29448. Forward Primer - name:029448_F_IRAV63-66_B22_Actr1b, sequence:TTGCCCCGAAGGATGTTA; Reverse Primer - name:029448_R_SP6_IRAV63-66_B22_Actr1b, sequence:GCCCTGGTCATGGTGAAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18381 same embryo
	EMAGE:18380 same embryo
	EMAGE:18384 same embryo
	EMAGE:18383 same embryo
	EMAGE:18382 same embryo
	EurExpress:euxassay_014307 same experiment
	MGI:4822960 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source