EMAGE mouse gene expression spatial database

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EMAGE:18387

Snrnp70 small nuclear ribonucleoprotein 70 (U1) ( MGI:98341)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18387_wholemount_strong.wlz

18387_wholemount_moderate.wlz

18387_wholemount_weak.wlz

18387_wholemount_possible.wlz

18387_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18387_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30764
Entity Detected:	Snrnp70, small nuclear ribonucleoprotein 70 (U1) ( MGI:98341)
Sequence:	sense strand is shown >T30764 GGACTGTGAAGGGCTGGAGGCCCCGGCGCCTAGGAGGTGGCCTTGGTGGTACCAGAAGAGGTGGGGCAGA TGTGAATATCAGGCATTCGGGCCGAGATGACACTTCCCGATATGATGAGAGGCCGGGCCCCTCCCCGCTT CCGCTCTCGGGACCGGAGGAGGCGCTCCCGGAGTCGAGACAAGGATGAGCGGCGACGGTCCAGGGAGCGT AGCAAGGACAAGGACCGAGACCGAAAGCGACGAAGCAGTCGGAGCCGGGAGCGAGCACGCAGGGAACGGG AGCGAAAAGAGGAGCTTCGAGGTGGAGGAGGTGGTGGCGGTGGTGGCAGTGGTGGTGGTGGCGGCGGCGA CATGGCTGAACCCTCTGAAGCTGGTGATGGAGCTCCTGATGATGGGCCTCCTGGAGAGCTTGGCCCTGAA GGCCCTGATGGCCCAGAGGAGAAGGGCCGGGATCGAGACCGGGAGAGGCGACGTAGTCACCGGAGTGAGA GGGAACGTCGTCGGGACCGAGACCGTGATCGGGATCGAGAGCACAAGCGTGGGGAGCGGGGCAGTGAGCG GGGCAGGGATGAGGCCCGGGGTGGGGGTGGCAGCGGTCAGGACAATGGGCTGGAAGGGCTAGGTAGTGAT GGTCGAGACATGTACATGGAGGCAGAGGGTGGTGATGGGTACATGGCCCCAGAGAATGGATATCTGATGG AGGCAGCGCCCGAATGAAGAGGCCTGTAGTGGACATGCTCCTAATCTTGCTGTCTGCCTTCCTAGCCTTT TCCACCTTGGCTTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3486572), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 13689. Forward Primer - name:013689_F_IRAV01-03_L13_Snrp70, sequence:GGACTGTGAAGGGCTGGA; Reverse Primer - name:013689_R_SP6_IRAV01-03_L13_Snrp70, sequence:GCAAGCCAAGGTGGAAAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18386 same embryo
	EMAGE:18390 same embryo
	EMAGE:18389 same embryo
	EMAGE:18388 same embryo
	EurExpress:euxassay_014321 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source