EMAGE mouse gene expression spatial database

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EMAGE:18410

Zcchc6 zinc finger, CCHC domain containing 6 ( MGI:2387179)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014361. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014361_01	euxassay_014361_02	euxassay_014361_03	euxassay_014361_04

euxassay_014361_05	euxassay_014361_06	euxassay_014361_07	euxassay_014361_08	euxassay_014361_09

euxassay_014361_10	euxassay_014361_11	euxassay_014361_12	euxassay_014361_13	euxassay_014361_14

euxassay_014361_15	euxassay_014361_16	euxassay_014361_17	euxassay_014361_18	euxassay_014361_19

euxassay_014361_20	euxassay_014361_21	euxassay_014361_22	euxassay_014361_23	euxassay_014361_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18410_wholemount_strong.wlz

18410_wholemount_moderate.wlz

18410_wholemount_weak.wlz

18410_wholemount_possible.wlz

18410_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18410_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 10 11 14 15 17 18 moderate expression: see section 01 02 03 04 05 06 07 08 09 16 19 20 21 22 23

radius

strong

regional

strong expression: see section 01 23 24

humerus

strong

regional

strong expression: see section 01 02 03 04 20 21 22 23 24 moderate expression: see section 05

forelimb digit 2 phalanx

strong

regional

strong expression: see section 02

forelimb digit 3 metacarpal

moderate

regional

moderate expression: see section 24

forelimb digit 3 phalanx

moderate

regional

moderate expression: see section 24

forelimb digit 4 phalanx

moderate

regional

moderate expression: see section 24

carpus

strong

regional

strong expression: see section 02

foot

strong

regional

strong expression: see section 07 11 12

fibula

strong

regional

strong expression: see section 01 moderate expression: see section 02 03 04

tibia

strong

regional

strong expression: see section 01 05 moderate expression: see section 03 04

femur

strong

regional

strong expression: see section 01 05 06 07 17 19 20 21 22 moderate expression: see section 02 03 04 18 23

otic capsule

strong

regional

strong expression: see section 07 08 09 15 16 17 18 19

viscerocranium

strong

regional

Expression in the turbinate bone.

hyoid

moderate

regional

moderate expression: see section 09 13 14 weak expression: see section 10 11 15

meckel's cartilage

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

axial skeleton

strong

regional

strong expression: see section 10 11 12 13 14 15 17 moderate expression: see section 07 08 09 16

basioccipital bone

strong

regional

strong expression: see section 01 02 03 09 10 13 14 15 16 17 18 19 20 21 22 23 24 moderate expression: see section 04 05 06 07 08 11 12

basisphenoid bone

strong

regional

strong expression: see section 09 10 13 14 15 16 17 moderate expression: see section 08 11 12

temporal bone petrous part

strong

regional

strong expression: see section 01 02 03 18 19 20 21 22 23 moderate expression: see section 04 05

vault of skull

strong

regional

strong expression: see section 24

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 05 06 07 08 17 18 19 20 21 22 23 24 moderate expression: see section 04

clavicle

moderate

regional

moderate expression: see section 06 07 16 18 19

scapula

strong

regional

strong expression: see section 02 03 04 20 21 22 23 moderate expression: see section 05

pelvic girdle skeleton

strong

regional

strong expression: see section 09 15 moderate expression: see section 08 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31521
Entity Detected:	Zcchc6, zinc finger, CCHC domain containing 6 ( MGI:2387179)
Sequence:	sense strand is shown >T31521 AACTGGCCCCAAATGACAGGTGCTGCCGAATTTGTGGGAAAATCGGGCACTTCATGAAGGACTGTCCCAT GAGGAGGAAAGTGAGGCGACGGAGAGACCAGGAAGATACCCCAAACCAAAGGTACTCTGAGAGCAAAGAA AAAAGAAGCAAAGAGGACAAAGAGATTCAGAACAAGTACACAGAAAAGGAAGTGTCAACAAAAGAAGATA AGCTCACACCGTGTGCAGCTGCGAAAGCCAAGCCAGTGAGGGCAGCTGTCGACCTGGGCCGGGAGAAGCT CCTCAGGACGCCCACAGAAAAGTGGAAGAGACAGGACGACAGAGACTCGAGAGAAAAGCGTTGTTTCATC TGTGGAAGAGAAGGACACATTAAAAAGGAATGCCCACAGTTCAAAGGCTCTCCAGGTAGCCTTTCCAGTA AATATATGACTCAGGGGAGAGCCTCAGTGAAGAGGACCCAGCAGGAATCATGAGGGAAGGAACTGCAGCA CTGGAAATGCCACTCAGGTGTCCGTGGCCACTTGGGAAATTAGGTTCATGGCACAGGACACAGTGACACA GATCAGGCTTCAACGTAACAGTTGAGGGGCATCGTAATTTTTTAAATTTTAATGAAATTGTTAATAAGGA AAAGAAAAATTTTAATATAGTCTAACTATACCACCAATCTCCAGAGATTTGAAGATTTTAATAGAAAGTC AACTTAAAAGAAAAATATGAGGGCCAGAATTCAATGACTACAGCGTCATGGATTTTAGTTCTTTCAAAGC ACTCTAAAAATAGTGTGCTGCTCACCAGAGGAGTTAGCCCTCTTTCCCTACACATCCCCTTTTTGATTTC CAAGAGGGGATTCTCTCTGTTGTTCCTGGATCGTGGTATGGACG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5354757), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 31839. Forward Primer - name:031839_F_IRAV67-70_B09_Zcchc6, sequence:AACTGGCCCCAAATGACA; Reverse Primer - name:031839_R_SP6_IRAV67-70_B09_Zcchc6, sequence:GCGTCCATACCACGATCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18409 same embryo
	EMAGE:18411 same embryo
	EurExpress:euxassay_014361 same experiment
	MGI:4829276 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source