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EMAGE:18414

1110017D15Rik RIKEN cDNA 1110017D15 gene ( MGI:1920971)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18414 EMAGE:18414 EMAGE:18414 EMAGE:18414 EMAGE:18414
"Pseudo-wholemount" of euxassay_014364. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014364_01 euxassay_014364_02 euxassay_014364_03 euxassay_014364_04
EMAGE:18414 EMAGE:18414 EMAGE:18414 EMAGE:18414 EMAGE:18414
euxassay_014364_05 euxassay_014364_06 euxassay_014364_07 euxassay_014364_08 euxassay_014364_09
EMAGE:18414 EMAGE:18414 EMAGE:18414 EMAGE:18414 EMAGE:18414
euxassay_014364_10 euxassay_014364_11 euxassay_014364_12 euxassay_014364_13 euxassay_014364_14
EMAGE:18414 EMAGE:18414 EMAGE:18414 EMAGE:18414 EMAGE:18414
euxassay_014364_15 euxassay_014364_16 euxassay_014364_17 euxassay_014364_18 euxassay_014364_19
EMAGE:18414
euxassay_014364_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18414Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18414_wholemount_strong.wlz
18414_wholemount_moderate.wlz
18414_wholemount_weak.wlz
18414_wholemount_possible.wlz
18414_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18414_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
radius
strong strong
regionalstrong expression: see section 01
humerus
strong strong
regionalstrong expression: see section 02 03 04 05 15 16 17 18 19 20
forelimb digit 2 metacarpal
strong strong
regionalstrong expression: see section 04 05
forelimb digit 2 phalanx
strong strong
regionalstrong expression: see section 02 03
forelimb digit 3 phalanx
strong strong
regionalstrong expression: see section 03
hindlimb digit 2 phalanx
strong strong
regionalstrong expression: see section 05 19
hindlimb digit 3 phalanx
strong strong
regionalstrong expression: see section 05 19
hindlimb digit 4 phalanx
strong strong
regionalstrong expression: see section 05 19 20
tarsus
strong strong
regionalstrong expression: see section 04 05
fibula
strong strong
regionalstrong expression: see section 03
tibia
strong strong
regionalstrong expression: see section 02 03
femur
strong strong
regionalstrong expression: see section 03 04 05 06 09 10 16 17 18 19 20
otic capsule
strong strong
regionalstrong expression: see section 06 07 08 09 13 14
viscerocranium
strong strong
regionalExpression in the turbinate bone.
hyoid
strong strong
regionalstrong expression: see section 10 11 12
meckel's cartilage
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18
axial skeleton
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15
basioccipital bone
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
basisphenoid bone
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14
temporal bone petrous part
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 15 16 17 18 19 20
vault of skull
strong strong
regionalstrong expression: see section 09 10 11 12 13 14
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 15 16 17 18 19 20
clavicle
strong strong
regionalstrong expression: see section 06 07 08 09 13 14
scapula
strong strong
regionalstrong expression: see section 02 03 17 18 19
pelvic girdle skeleton
strong strong
regionalstrong expression: see section 11 12 14 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31846
Entity Detected:1110017D15Rik, RIKEN cDNA 1110017D15 gene ( MGI:1920971)
Sequence:sense strand is shown

>T31846
CACGTGTGCCACTTCCAGGACACCGGGATGGAGTCAGTTCGAGGAATGCCGTTGGAATATCCTCCTAAGC
AGGAGCGCCTCAACGCCTACGATTGTACCCCGCTGTGGGTGCGTGGACCCTCTCCCGGGCCGATTGCCAT
ACCAAGGATATGAAAGCCCTTGCTCCGGCCGCCACTACTGTCTGCGCGGGATGGACTACTGCACCACCCG
GGAGCCTAGCACAGAACGCCGCCTGCGGCCTTTGTGCTCGCAGCAGCCGACTGAGTGTGTCGCCCTTCGG
TCACCGGCCAGGAATGCAATGTGCTGTTACAACTCCCCCGCCATCATTTTACCCGTATCCCAACCTTAGA
TGGGACACAAGTCACTTCAAGAAGACTGGTGGTTCCCAGAGAAACAACTATGTAGTCCATCCTGAGTTTG
TGTCTGAGACCTGTCCTGTCTACCCTTCCTAGTAAAGCTTTGACCAGGCCAAGGGAGGAAGTGGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6741413), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 63549. Forward Primer - name:063549_F_IRAW2_a10_1110017D15Rik, sequence:CACGTGTGCCACTTCCAG; Reverse Primer - name:063549_R_SP6_IRAW2_a10_1110017D15Rik, sequence:AGCCACTTCCTCCCTTGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18417 same embryo
 EMAGE:18416 same embryo
 EMAGE:18415 same embryo
 EMAGE:18413 same embryo
 EurExpress:euxassay_014364 same experiment
 MGI:4822593 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS