EMAGE mouse gene expression spatial database

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EMAGE:18526

Stx16 syntaxin 16 ( MGI:1923396)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014483. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014483_01	euxassay_014483_02	euxassay_014483_03	euxassay_014483_04

euxassay_014483_05	euxassay_014483_06	euxassay_014483_07	euxassay_014483_08	euxassay_014483_09

euxassay_014483_10	euxassay_014483_11	euxassay_014483_12	euxassay_014483_13	euxassay_014483_14

euxassay_014483_15	euxassay_014483_16	euxassay_014483_17	euxassay_014483_18	euxassay_014483_19

euxassay_014483_20	euxassay_014483_21	euxassay_014483_22	euxassay_014483_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18526_wholemount_strong.wlz

18526_wholemount_moderate.wlz

18526_wholemount_weak.wlz

18526_wholemount_possible.wlz

18526_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18526_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

strong

single cell

strong expression: see section 05 moderate expression: see section 18 19

glossopharyngeal ix ganglion

strong

single cell

strong expression: see section 06 07

trigeminal v ganglion

strong

single cell

strong expression: see section 03 04 05 06 07 08 moderate expression: see section 17 18 19 20 21

vestibulocochlear viii ganglion

strong

single cell

strong expression: see section 06 07 moderate expression: see section 17

dorsal root ganglion

moderate

single cell

moderate expression: see section 06 07 08 09 17

neural retina

weak

regional

weak expression: see section 03 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38024
Entity Detected:	Stx16, syntaxin 16 ( MGI:1923396)
Sequence:	sense strand is shown >T38024 TGGATGATGGAGATGATGCTACTCTGTATGGTCAGGGCTTCACGGATGACCAGCTGGTGCTGGTAGAGCA AAACACACTGATGGTGGAGGAGAGGGAGCGAGAGATCCGTCAGATTGTGCAGTCCATTTCAGACCTCAAT GAAATCTTCAGGGACTTGGGAGCCATGATTGTGGAGCAGGGAACAGTCCTTGATAGAATTGACTATAATG TTGAACAGTCCTGTGTCAAGACCGAAGATGGCTTGAAACAGCTTCACAAGGCTGAGCAGTACCAGAAGAA GAACCGGAAGATGCTTGTGATCGTGATCCTGGTGGCCGTCATCATAGTCCTCCTCGTGGCCCTCGTCGCC GTAAAGTCTCGCTAGTGGCACCAGGTTCCCGAGAGTGCTACTGTGTGGATTATTGGTGATAGGACTTGGC TGGTGCCTCACCGCTGCACTGTGTAAAGAGCACAGCCCACTGGTCCATCAGTGGGCAGCAGACCTTCCCG TGGACACCTTCTAACCAGTGGGCTGGCGTGTAGGCACGGCCATGTTTTCCATCTCTGTCCAGAGTCTAAG GACTTGATGCTGTTGCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 75047. Forward Primer - name:075047_F_cDNA_Stx16, sequence:TGGATGATGGAGATGATGCTAC; Reverse Primer - name:075047_N_SP6_cDNA_Stx16, sequence:GGCAACAGCATCAAGTCCTTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18523 same embryo
	EMAGE:18525 same embryo
	EMAGE:18527 same embryo
	EMAGE:18524 same embryo
	EurExpress:euxassay_014483 same experiment
	MGI:4828523 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source