EMAGE mouse gene expression spatial database

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EMAGE:18607

Msi2 Musashi homolog 2 (Drosophila) ( MGI:1923876)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014584. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014584_01	euxassay_014584_02	euxassay_014584_03	euxassay_014584_04

euxassay_014584_05	euxassay_014584_06	euxassay_014584_07	euxassay_014584_08	euxassay_014584_09

euxassay_014584_10	euxassay_014584_11	euxassay_014584_12	euxassay_014584_13	euxassay_014584_14

euxassay_014584_15	euxassay_014584_16	euxassay_014584_17	euxassay_014584_18	euxassay_014584_19

euxassay_014584_20	euxassay_014584_21	euxassay_014584_22	euxassay_014584_23	euxassay_014584_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18607_wholemount_strong.wlz

18607_wholemount_moderate.wlz

18607_wholemount_weak.wlz

18607_wholemount_possible.wlz

18607_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18607_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38812
Entity Detected:	Msi2, Musashi homolog 2 (Drosophila) ( MGI:1923876)
Sequence:	sense strand is shown >T38812 CTCGATTGCTGTCGACTTAATGTTCCACTGCACAGCAATTCACGGCCAGTGAATGTTACACACATCTTGC TAGACTAGTATAAAAATCATTGGGTAATTGTTGGTTCTAATGACCTGAAAGGTGTTCAGTTTGTGGGTTT TTTTTGGGGGGGGCTGCTTGGGGGGTTGTTTTTCCTTCTTTGTTTTTTTTTAATTTGAGAATTTAGGGGG ATAATTTTTGGGGGGGGGTTCCAAATAAAAAAATAGAAAGCTATTTTGATCTTTAGTGCAAACTAGGGAC GTAGCCTCCATCACCACAACCACACCGCTTCGCTCTGCCATCCACCCACCGCAGCAGCATCTTCAAGAAT AAAGCAATATAGTTTACTACATTTTTTTTTAAAATTGAAGGTCAGCCATGCTTTCTGTATTATATTGCAT ATGAAATTGTTTACAAAAGAAACACTAACTCATCCTTCTCTTTATCAGTTGAAAGTGACACACGGGACAG AGGGAAATTGGAGGGCAGGTGAGGGAGGGGAGAGGGTTTTTTTGTATTTTGGTTTGTTTGGTTTTGGTTT CTTTTCTTGAATGTGCTTTAGTAGCCAGATCCTCCAAGGAAGGCAAAAGCCAGCCAGCCAGAGAGAGAGT AGCGAAGCGGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 236262. Forward Primer - name:165417_F_cDNA_Msi2h, sequence:CTCGATTGCTGTCGACTTAATG; Reverse Primer - name:165417_N_SP6_cDNA_Msi2h, sequence:CCCGCTTCGCTACTCTCTCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18606 same embryo
	EMAGE:18609 same embryo
	EMAGE:18605 same embryo
	EMAGE:18608 same embryo
	EMAGE:18604 same embryo
	EurExpress:euxassay_014584 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source