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EMAGE:18608

Pag1 phosphoprotein associated with glycosphingolipid microdomains 1 ( MGI:2443160)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18608 EMAGE:18608 EMAGE:18608 EMAGE:18608 EMAGE:18608
"Pseudo-wholemount" of euxassay_014585. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014585_01 euxassay_014585_02 euxassay_014585_03 euxassay_014585_04
EMAGE:18608 EMAGE:18608 EMAGE:18608 EMAGE:18608 EMAGE:18608
euxassay_014585_05 euxassay_014585_06 euxassay_014585_07 euxassay_014585_08 euxassay_014585_09
EMAGE:18608 EMAGE:18608 EMAGE:18608 EMAGE:18608 EMAGE:18608
euxassay_014585_10 euxassay_014585_11 euxassay_014585_12 euxassay_014585_13 euxassay_014585_14
EMAGE:18608 EMAGE:18608 EMAGE:18608 EMAGE:18608 EMAGE:18608
euxassay_014585_15 euxassay_014585_16 euxassay_014585_17 euxassay_014585_18 euxassay_014585_19
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euxassay_014585_20 euxassay_014585_21 euxassay_014585_22 euxassay_014585_23 euxassay_014585_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18608Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18608_wholemount_strong.wlz
18608_wholemount_moderate.wlz
18608_wholemount_weak.wlz
18608_wholemount_possible.wlz
18608_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18608_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38810
Entity Detected:Pag1, phosphoprotein associated with glycosphingolipid microdomains 1 ( MGI:2443160)
Sequence:sense strand is shown

>T38810
TGTCACCAGGCTCTAGCAACTAAAAGAGGCTGTGATCAGCAGCTCTGCTGTGGAAAGGAGGTGGCTGCAG
TTTCTCGCTGCTTTGGTTGACTGAAATCTTCAAGTGCAGTCTCCCAGTTGTCTCCCAGTTTCCAAAACAG
TAAGGTACGGACCTGCCTGCCAGGGCAGCCACCCTCCAGTGCTCCCAAGAAACCCCTGGGACCCCTCACC
TCCACATTCCACAGAGGAAGGCCAGCTGTGGGTGCTGGCGGGGAAGCCCCACCCACCAATGCCAAAATCC
CACTGCCTGCTGAAGAAGAGCCAGGTTTCCATCCTTGCTCTCTTTCCCTCTGCTCCTCATGCTAAAACAC
TGGCACTTCACCCCTGGAGACCTCCCATGTGTGCTTCATCCCAAGGATGGTCTCCCACTGGCCTGCACTG
GCCTTCAGTAAATGTTAGCATGGAGAGAAACTGGTTGCCGTTAACCCACCCTCTTCCCAGACCTGCCTCT
TAGTCCCTGGGAAGGAACCTGGATGTGCAGGCTGTGAGGTATGGGGTGGGCCGTCCCTGGAGTCTCTGGG
GTTCTTGCTCCTCGTTGGACACTGCATCTGCAGTCTTCAGAGGTTCATGAGGTCAGTTGAGATGTTTTTT
AATGTTCTATGTTGAAAACCTTCCCAACAGTGTTTGGATTTTCATAACTACTGTGGTACTTCTGTAGCTG
TTCACACCCAGTGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 236027. Forward Primer - name:165182_F_cDNA_Pag, sequence:TGTCACCAGGCTCTAGCAACTA; Reverse Primer - name:165182_N_SP6_cDNA_Pag, sequence:GCACTGGGTGTGAACAGCTAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18606 same embryo
 EMAGE:18609 same embryo
 EMAGE:18605 same embryo
 EMAGE:18607 same embryo
 EMAGE:18604 same embryo
 EurExpress:euxassay_014585 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS