EMAGE mouse gene expression spatial database

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EMAGE:18719

Gm5433 predicted gene 5433 ( MGI:3649058)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014091. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014091_01	euxassay_014091_02	euxassay_014091_03	euxassay_014091_04

euxassay_014091_05	euxassay_014091_06	euxassay_014091_07	euxassay_014091_08	euxassay_014091_09

euxassay_014091_10	euxassay_014091_11	euxassay_014091_12	euxassay_014091_13	euxassay_014091_14

euxassay_014091_15	euxassay_014091_16	euxassay_014091_17	euxassay_014091_18	euxassay_014091_19

euxassay_014091_20	euxassay_014091_21	euxassay_014091_22	euxassay_014091_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18719_wholemount_strong.wlz

18719_wholemount_moderate.wlz

18719_wholemount_weak.wlz

18719_wholemount_possible.wlz

18719_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18719_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

weak

single cell

weak expression: see section 05 06 07 08 09 10 12 13 14 16

medulla oblongata basal plate mantle layer

weak

single cell

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

pons mantle layer

weak

single cell

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain mantle layer

weak

single cell

weak expression: see section 05 06 07 08 09 10 14 16 17

facial vii ganglion

moderate

single cell

moderate expression: see section 03 04 20

trigeminal v ganglion

moderate

single cell

moderate expression: see section 03 04 05 06 07 16 17 18 19 20 21

ventral grey horn

moderate

single cell

moderate expression: see section 18 weak expression: see section 12 13 14 16 17

dorsal root ganglion

moderate

single cell

moderate expression: see section 11 12 14 16 19 20 weak expression: see section 09 13 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38779
Entity Detected:	Gm5433, predicted gene 5433 ( MGI:3649058)
Sequence:	sense strand is shown >T38779 AGTCCTAGGTCCTCCTGCTCTTTAGAACAGACTCTGGGGCAAACCAGTGCTTTGTTCAGTAAGAACATTG CAAGTATGATCATTGCTGTGAATGGAGAGACTGCACCATAAGGGGAAACAGAGACAGTGCTGTAGCCTAG GAAGAGAAGTTGTGCTCGGGTGACAGGACTTTCCTGTCCCATTCTGACTGCCACTTAGCTCTTGAGTCAA TTACTAGCCTCCCTGTGAGCCCCATCTTCCATCTCGAGGAAATGGAGGTAACACTGGGCCTCCCTCCTTG GTCTGTCCTGAGGATCTCAGGGGACAATGACAGCTTTCAGTGAGGTACAAGACTATTTAGGAACAGTCCC ATAATACTGTTCACTGAGAAGAGGCCTCCAGTCCCCATGTTGGGAACAGAAGTAGGAAAGAGATGGCCTT CTGAGCTGGCTCTACAAGGGTGGCTAAGTTATATTCATCATGCTGACTGTGTGTATCTTTGACCAGATTG GTGATGGTAGTGTGCTGTCTGAGTTGGGATATATTTTTACTCCATGTGTACATTTACTTTGTGCCATTTT CTGAGCCAGCGAAGCACGTCTGGGCAGTATGGGCTTTGAGGAAAAGGCCTGGTTCAGGTCACT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 235622. Forward Primer - name:164777_F_cDNA_LOC432637, sequence:AGTCCTAGGTCCTCCTGCTCTT; Reverse Primer - name:164777_N_SP6_cDNA_LOC432637, sequence:AGTGACCTGAACCAGGCCTTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18720 same embryo
	EMAGE:18722 same embryo
	EMAGE:18721 same embryo
	EMAGE:18723 same embryo
	EMAGE:18718 same embryo
	EurExpress:euxassay_014091 same experiment
	MGI:4825108 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source