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EMAGE:18949

Chmp6 charged multivesicular body protein 6 ( MGI:3583942)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18949 EMAGE:18949 EMAGE:18949 EMAGE:18949 EMAGE:18949
"Pseudo-wholemount" of euxassay_014176. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014176_01 euxassay_014176_02 euxassay_014176_03 euxassay_014176_04
EMAGE:18949 EMAGE:18949 EMAGE:18949 EMAGE:18949 EMAGE:18949
euxassay_014176_05 euxassay_014176_06 euxassay_014176_07 euxassay_014176_08 euxassay_014176_09
EMAGE:18949 EMAGE:18949 EMAGE:18949 EMAGE:18949 EMAGE:18949
euxassay_014176_10 euxassay_014176_11 euxassay_014176_12 euxassay_014176_13 euxassay_014176_14
EMAGE:18949 EMAGE:18949 EMAGE:18949 EMAGE:18949 EMAGE:18949
euxassay_014176_15 euxassay_014176_16 euxassay_014176_17 euxassay_014176_18 euxassay_014176_19
EMAGE:18949 EMAGE:18949 EMAGE:18949 EMAGE:18949
euxassay_014176_20 euxassay_014176_21 euxassay_014176_22 euxassay_014176_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18949Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18949_wholemount_strong.wlz
18949_wholemount_moderate.wlz
18949_wholemount_weak.wlz
18949_wholemount_possible.wlz
18949_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18949_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40441
Entity Detected:Chmp6, charged multivesicular body protein 6 ( MGI:3583942)
Sequence:sense strand is shown

>T40441
CGCCTTGAAGTTTAGTCTGGATGCACCTTTGTCTCTTTCTCCTTCTGGACTAAGTGGATCCCCGGCCAGC
CAGGCCCAGCTTTAGGCAGCTGTGGACCCCATGCTGGCCGCAGCCCACTACTCCTGGTGCTGTTCTCATG
CTGCCCCAGCCCATGTGAGCTGCGCTCAGACCTCGGGGTCCCTGTGCTCCCCTCTCGTGTCTCTGCTGCT
CACATGAGCTGGTGGGGGCTTCAGCTGAGCAGTTCTCGCCTTGCCTGGCATCCTGACACCCCTCAGTTAC
CCTGCAGGCTAGTGGCAGAAACCCTAGTGTTCTCCAAATCTGTTGGAGCATCTTTGCACCTCAGATCACC
TTGAGCCTCTTCTGTTGGGGGAGGTGGGCCAGGGGCCATCCCACAGAATGGACAAATCTAGGAGCCCGGC
TTGGGGTCTGTTCGCAGAAGAAAAACATGTTGAAGGTGGTAACAAGACCCTGTTTTAATGGGCATGTACC
CCATGGAGTCCTGGACACACTAAGCCACCAACGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 161627. Forward Primer - name:161627_F_cDNA_mCG119194, sequence:CGCCTTGAAGTTTAGTCTGGAT; Reverse Primer - name:161627_N_SP6_cDNA_mCG119194, sequence:GCGTTGGTGGCTTAGTGTGTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18950 same embryo
 EMAGE:18947 same embryo
 EMAGE:18946 same embryo
 EMAGE:18948 same embryo
 EurExpress:euxassay_014176 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS