EMAGE mouse gene expression spatial database

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EMAGE:18969

Man1c1 mannosidase, alpha, class 1C, member 1 ( MGI:2446214)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_015930. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_015930_01	euxassay_015930_02	euxassay_015930_03	euxassay_015930_04

euxassay_015930_05	euxassay_015930_06	euxassay_015930_07	euxassay_015930_08	euxassay_015930_09

euxassay_015930_10	euxassay_015930_11	euxassay_015930_12	euxassay_015930_13	euxassay_015930_14

euxassay_015930_15	euxassay_015930_16	euxassay_015930_17	euxassay_015930_18	euxassay_015930_19

euxassay_015930_20	euxassay_015930_21	euxassay_015930_22	euxassay_015930_23	euxassay_015930_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18969_wholemount_strong.wlz

18969_wholemount_moderate.wlz

18969_wholemount_weak.wlz

18969_wholemount_possible.wlz

18969_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18969_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rest of cerebellum marginal layer

weak

regional

weak expression: see section 05 06 07 09 10 11 12 13 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T63352
Entity Detected:	Man1c1, mannosidase, alpha, class 1C, member 1 ( MGI:2446214)
Sequence:	sense strand is shown >T63352 CTGCTTTTAATGCCTATCACCCGACATTGATTCTGTCCCACTTCTTGTCATCTACCCTGTCATCGGAGGA GACAGTCCCCTTTGCTTTGGTCCCAAATGTTACAAATCACTAATAATGCTATTTTCATTACTGTAATGGA GAAATATGTGAAGTAGAATAAAGGATTTCATTGAATAAGAAATCAGATTGGACTCATTGGCCACCAGAGG CTCCTGGGAGAAAAATCCTCCAACAGTGTCACCCGAAGCCGTCACCAGCTGGCAGAGGAGCTGAGGTGTG ATATACTAAAGGCAGTGGGTGCAGCTTCTCTGTCTCTCTCCAGGTGGCCCTAGTAAGTGCAGGAAATGAG AGGCCAGCCCTTTGGGTAGAGTTGGGTATGAATACTCACACTCGGATGGAGGAGTGGAAGAAGCCCAGGG AGGTGACTCTGTTTGCCTGAAGTCACCTTGCCTCTGGGATCTCCTGGTCACCCTGGTCCTTTGTTCATGT TGTCAATCAGCAATGCTTAGTGAGCCCCTGCCCCCACCAGGGCTAGATGGCCTCCACCTCGCAGTCAGGG CGCTCAGATCATATACGATGCCTCTACATTAGTGGAATGACTATAAACCATTGCCAATACTAACAAGGAT GCAGGAGGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 75142. Forward Primer - name:075142_F_cDNA_Man1c1, sequence:CTGCTTTTAATGCCTATCACCC; Reverse Primer - name:075142_N_SP6_cDNA_Man1c1, sequence:GCCTCCTGCATCCTTGTTAGTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18970 same embryo
	EMAGE:18973 same embryo
	EMAGE:18974 same embryo
	EMAGE:18975 same embryo
	EMAGE:18976 same embryo
	EMAGE:18972 same embryo
	EMAGE:18971 same embryo
	EurExpress:euxassay_015930 same experiment
	MGI:4826073 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source