EMAGE mouse gene expression spatial database

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EMAGE:19205

Vapb vesicle-associated membrane protein, associated protein B and C ( MGI:1928744)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001887. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001887_01	euxassay_001887_02	euxassay_001887_03	euxassay_001887_04

euxassay_001887_05	euxassay_001887_06	euxassay_001887_07	euxassay_001887_08	euxassay_001887_09

euxassay_001887_10	euxassay_001887_11	euxassay_001887_12	euxassay_001887_13	euxassay_001887_14

euxassay_001887_15	euxassay_001887_16	euxassay_001887_17	euxassay_001887_18	euxassay_001887_19

euxassay_001887_20	euxassay_001887_21	euxassay_001887_22	euxassay_001887_23	euxassay_001887_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19205_wholemount_strong.wlz

19205_wholemount_moderate.wlz

19205_wholemount_weak.wlz

19205_wholemount_possible.wlz

19205_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19205_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1199
Entity Detected:	Vapb, vesicle-associated membrane protein, associated protein B and C ( MGI:1928744)
Sequence:	sense strand is shown >T1199 ANNCTGTTGGCCTACTGGTGATGCCGGGGCCTCTCTCAATGTGTCTGTGATGTTACAGCCTTTCGATTAT GATCCCAATGAGAAAAGTAAACACAAGTTTATGGTTCAGTCTATGTTTGCTCCGCCTGACACTTCTGATA TGGAGGCAGTATGGAAGGAGGCAAAACCGGAAGACCTTATGGATTCAAAACTTAGATGTGTGTTTGAATT GCCAGCAGAAAATGCTAAACCACATGATGTAGAAATAAATAAAATCATACCTACGAGTGCATCCAAGACA GAAGCACCGGCAGCCGCAAAGTCCCTGACATCGCCCCTCGATGACACAGAAGTAAAGAAGGTGATGGAAG AGTGCAGGCGGCTGCAGGGGGAGGTGCAGAGGCTTCGGGAGGAGAGCAGGCAGCTCAAGGAAGAAGACGG ACTTCGGGTGAGGAAGGCGATGCCGAGCAACAGCCCCGTGGCGGCTCTGGCGGCCACTGGGAAGGAGGAG GGCCTGAGCGCCCGGCTGCTGGCCCTG
Notes:	The probe template was PCR amplified from IMAGE:2182368 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2182368 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19208 same embryo
	EMAGE:19207 same embryo
	EMAGE:19206 same embryo
	EurExpress:euxassay_001887 same experiment
	MGI:4829147 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source