EMAGE mouse gene expression spatial database

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EMAGE:19206

Snx31 sorting nexin 31 ( MGI:1913946)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009174. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009174_01	euxassay_009174_02	euxassay_009174_03	euxassay_009174_04

euxassay_009174_05	euxassay_009174_06	euxassay_009174_07	euxassay_009174_08	euxassay_009174_09

euxassay_009174_10	euxassay_009174_11	euxassay_009174_12	euxassay_009174_13	euxassay_009174_14

euxassay_009174_15	euxassay_009174_16	euxassay_009174_17	euxassay_009174_18	euxassay_009174_19

euxassay_009174_20	euxassay_009174_21	euxassay_009174_22	euxassay_009174_23	euxassay_009174_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19206_wholemount_strong.wlz

19206_wholemount_moderate.wlz

19206_wholemount_weak.wlz

19206_wholemount_possible.wlz

19206_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19206_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand

moderate

regional

moderate expression: see section 03 04 05 06 24

foot

moderate

regional

moderate expression: see section 01 24

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 04 05 19 20 21 22 23 24

conjunctival sac

strong

regional

strong expression: see section 04 05 22

anterior naris

strong

regional

strong expression: see section 11 12 13 15 16

esophagus

moderate

regional

moderate expression: see section 12 13

stomach

moderate

regional

moderate expression: see section 04 05 06 07

oral epithelium

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16

urinary system

strong

regional

strong expression: see section 13 14

bladder

strong

regional

strong expression: see section 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1198
Entity Detected:	Snx31, sorting nexin 31 ( MGI:1913946)
Sequence:	sense strand is shown >T1198 CCTCGAGNCTGTTGGCCTACTGGCCCAGACTCTAGGCGGCAGGTCCTGTTCTCCACGCTGTCCCTGGTCA TCGCCAGAGCCATGAAGATGCACTTCTGCATCCCGGTGTCCCAGCAGCGGCCAGACGCGCTGGGGGGCCG CTACGTGTTGTACTCCGTGTACCTGGACGGGTTCCTCTTCTGCAAAGTACGCTACAGCCAGCTGCACCGC TGGGATGAACAGCTGAGACGGGTGTTTGGAAACTGCCTGCCCCCTTTCCCACCCAAGTACTACCTGGCAA TGACTACTGCTATGGCTGAAGAGAGGAGGGACCAGTTGAGAACGCTACCTGCAAAATGTAACTGCGGATC CCCGTGTGACAAGGAGTGATGTCTTCACTGAGTTTTTAACACTGGTGCAGCTGCACACATTGAATATCAC CATCCAGAATGTCGAGCTGGCTGTATTCCTGCCTGATGGAAGGAGCATTAAAGTAGAAGGTCTAACATCA GATACGGCTGAAAGGGTCCTGGAGGTGATGGCCCACAAACTCGGATTGCAGCCAGATCTGGTGGGCTACT TCGG
Notes:	The probe template was PCR amplified from IMAGE:2182357 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2182357 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19208 same embryo
	EMAGE:19207 same embryo
	EMAGE:19205 same embryo
	EurExpress:euxassay_009174 same experiment
	MGI:4828368 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source