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EMAGE:19216

Fgd4 FYVE, RhoGEF and PH domain containing 4 ( MGI:2183747)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19216 EMAGE:19216 EMAGE:19216 EMAGE:19216 EMAGE:19216
"Pseudo-wholemount" of euxassay_009166. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009166_01 euxassay_009166_02 euxassay_009166_03 euxassay_009166_04
EMAGE:19216 EMAGE:19216 EMAGE:19216 EMAGE:19216 EMAGE:19216
euxassay_009166_05 euxassay_009166_06 euxassay_009166_07 euxassay_009166_08 euxassay_009166_09
EMAGE:19216 EMAGE:19216 EMAGE:19216 EMAGE:19216 EMAGE:19216
euxassay_009166_10 euxassay_009166_11 euxassay_009166_12 euxassay_009166_13 euxassay_009166_14
EMAGE:19216 EMAGE:19216 EMAGE:19216 EMAGE:19216 EMAGE:19216
euxassay_009166_15 euxassay_009166_16 euxassay_009166_17 euxassay_009166_18 euxassay_009166_19
EMAGE:19216 EMAGE:19216 EMAGE:19216 EMAGE:19216 EMAGE:19216
euxassay_009166_20 euxassay_009166_21 euxassay_009166_22 euxassay_009166_23 euxassay_009166_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19216Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19216_wholemount_strong.wlz
19216_wholemount_moderate.wlz
19216_wholemount_weak.wlz
19216_wholemount_possible.wlz
19216_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19216_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
hand
weak weak
regionalweak expression: see section 02 03 04 05 21 22 23 24
pancreas
moderate moderate
spottedmoderate expression: see section 09 10 11 15 16 weak expression: see section 12
brain
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 18 19
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 06 07 17
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 16 17 18 19 20 21
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 07 16 17
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 06 07 08 16 17 18
trigeminal v nerve
moderate moderate
regionalmoderate expression: see section 08 15
spinal cord
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16
cervico-thoracic ganglion
moderate moderate
regionalmoderate expression: see section 09 15
cervical ganglion
moderate moderate
regionalmoderate expression: see section 08 16
thoracic ganglion
weak weak
regionalweak expression: see section 12
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18
retina
moderate moderate
regionalmoderate expression: see section 01 02
neural retina
moderate moderate
regionalmoderate expression: see section 21 22 23
nasal cavity olfactory epithelium
moderate moderate
spottedmoderate expression: see section 06 07 08 09 10 13 14 15 16 17
vomeronasal organ
moderate moderate
spottedmoderate expression: see section 10 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2423
Entity Detected:Fgd4, FYVE, RhoGEF and PH domain containing 4 ( MGI:2183747)
Sequence:sense strand is shown

>T2423
TGGCCTCGAGGCCAGATTCGGACGAGGGTGGATTGTTCCATGGAGTTGTGTTCACTGATGAGGCATCAAT
ACTTGTGCAAAGGTTACTCCACCTTGTACGACAAGCAACACAACCACAGAAGCTCAGGGAATAAATGGGA
GGACACCAGCAAAACGCTCCACTGCAAGTGCAAAGCCACAAGTGCCTCCAAAACCATTATATCTGCAGAA
TTCACCTTCATCCCATATTCCCCAAGTCCCCAGGCATAAAGCTTTACCTAGTGCAAAGCCAAGGATGGAG
GAGTCTAATCCGGCCCCTACTTCTTGTACCTCAAAAGGGAAGCATAGTAAGGTATCGGATCTCATCAGCC
ACTTTGAAGGAGGCAGTGTTTTGTCAAGTTACATTGATTTGCAGAAAGATTCTACTATGAACCTCAATAT
TCCTCAAACTCTAGGACAGCCTGGGTTAACCTCCTCACCTCCACGAAAATTTCTGCCCCAGCACTCTCCA
CAGAAGCAGGAAAACGACCCAGTGCTAATTGTTATTTAAAAAACTAAGAATTATTTTGATTT
Notes:The probe template was PCR amplified from IMAGE:1230943 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1230943 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19219 same embryo
 EMAGE:19220 same embryo
 EMAGE:19221 same embryo
 EMAGE:19218 same embryo
 EMAGE:19217 same embryo
 EMAGE:19214 same embryo
 EMAGE:19215 same embryo
 EurExpress:euxassay_009166 same experiment
 MGI:4824838 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS