EMAGE mouse gene expression spatial database

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EMAGE:19217

F2r coagulation factor II (thrombin) receptor ( MGI:101802)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009165. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009165_01	euxassay_009165_02	euxassay_009165_03	euxassay_009165_04

euxassay_009165_05	euxassay_009165_06	euxassay_009165_07	euxassay_009165_08	euxassay_009165_09

euxassay_009165_10	euxassay_009165_11	euxassay_009165_12	euxassay_009165_13	euxassay_009165_14

euxassay_009165_15	euxassay_009165_16	euxassay_009165_17	euxassay_009165_18	euxassay_009165_19

euxassay_009165_20	euxassay_009165_21	euxassay_009165_22	euxassay_009165_23	euxassay_009165_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19217_wholemount_strong.wlz

19217_wholemount_moderate.wlz

19217_wholemount_weak.wlz

19217_wholemount_possible.wlz

19217_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19217_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

spotted

moderate expression: see section 01 02 03 04 05 08 09 10 11 12 13 14 15 16 17 18 19 20 21 weak expression: see section 06 07 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3516
Entity Detected:	F2r, coagulation factor II (thrombin) receptor ( MGI:101802)
Sequence:	sense strand is shown >T3516 TGGCCTCGAGCCAGATTCGGACGAGGCCGAGCTACTCAGAAAGAAGTAGGCGACGGCGGGCGCCGGGCCG CGCGGGCAGCCTTGGGACAATGGGGCCCCGGCGCTTGCTGATCGTCGCCCTCGGCCTCAGCCTGTGCGGT CCCTTGCTGTCTTCCCGCGTCCCTATGAGCCAGCCAGAATCAGAGAGGACAGATGCTACGGTGAACCCCC GCTCATTCTTTCTAAGGAATCCCAGTGAAAATACATTTGAACTGGTCCCCCTGGGGGATGAGGAGGAGGA GGAGAAAAATGAAAGCGTCCTGCTGGAGGGTAGGGCAGTCTACTTAAATATAAGCCTCCCTCCTCACACG CCGCCTCCTCCCTTCATCTCCGAGGACGCCTCCGGATATCTGACCAGCCCCTGGCTGACGCTCTTCATGC CCTCCGTGTACACGATTGTGTTCATTGTCAGCCTTCCTCTGAACGTCCTGGCCATCGCAGTGTTCGTCTT GAGGATGAAGGTCAAGAAGCCGGCCGTGGTGTACATGCTGCACCTGGCCATGGC
Notes:	The probe template was PCR amplified from IMAGE:337258 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:337258 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19219 same embryo
	EMAGE:19220 same embryo
	EMAGE:19221 same embryo
	EMAGE:19218 same embryo
	EMAGE:19216 same embryo
	EMAGE:19214 same embryo
	EMAGE:19215 same embryo
	EurExpress:euxassay_009165 same experiment
	MGI:4824662 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source