EMAGE mouse gene expression spatial database

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EMAGE:19219

Ccr6 chemokine (C-C motif) receptor 6 ( MGI:1333797)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001868. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001868_01	euxassay_001868_02	euxassay_001868_03	euxassay_001868_04

euxassay_001868_05	euxassay_001868_06	euxassay_001868_07	euxassay_001868_08	euxassay_001868_09

euxassay_001868_10	euxassay_001868_11	euxassay_001868_12	euxassay_001868_13	euxassay_001868_14

euxassay_001868_15	euxassay_001868_16	euxassay_001868_17	euxassay_001868_18	euxassay_001868_19

euxassay_001868_20	euxassay_001868_21	euxassay_001868_22	euxassay_001868_23	euxassay_001868_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19219_wholemount_strong.wlz

19219_wholemount_moderate.wlz

19219_wholemount_weak.wlz

19219_wholemount_possible.wlz

19219_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19219_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2431
Entity Detected:	Ccr6, chemokine (C-C motif) receptor 6 ( MGI:1333797)
Sequence:	sense strand is shown >T2431 TGGCTCGAGCAGATTCGGACGAGGCGATCCGGGTTCATCCCTTTCCCTACAACAAACAACATCCCACAAC CTTCACTGCAGCTCCTGGCAGCTTGTGACAGTGGTGCTGGGTGCCCACGACCTGCTGAGCTCGGAGCCTG AGCAGCAGAAGTTCACCATCAGTCAGGTCTTCCAGAACAATTACAACCCCGAGGAGAACCTCAATGACGT GCTTCTCCTCCAGCTAAACCGGACAGCCTCCCTGGGCAAGGAGGTGGCGGTGGCTTCTCTGCCCCAGCAG GACCAGACTCTGTCCCAGGGCACCCAGTGCCTGGCCATGGGCTGGGGTCGCCTGGGCACCCAAGCACCCA CGCCCCGTGTGCTGCAGGAACTGAACGTCACGGTGGTCACCTTCCTATGCCGGGAACACAACGTGTGCAC GCTGGTGCCACGGAGGGCAGCAGGCATATGCTTCGGAGACTCGGGCGGCCCCTTGATCTGCAATGGCATT CTTCATGGAGTGGACTCCTTCGTGATCCGCGAATGCGCCTCCCTCCAGTTCCCTGATTTCTTCGCCCG
Notes:	The probe template was PCR amplified from IMAGE:1244224 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1244224 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19220 same assay
	EMAGE:19221 same embryo
	EMAGE:19218 same embryo
	EMAGE:19217 same embryo
	EMAGE:19216 same embryo
	EMAGE:19214 same embryo
	EurExpress:euxassay_001868 same experiment
	EMAGE:19215 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source