EMAGE mouse gene expression spatial database

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EMAGE:19361

Sdc2 syndecan 2 ( MGI:1349165)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002176. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002176_01	euxassay_002176_02	euxassay_002176_03	euxassay_002176_04

euxassay_002176_05	euxassay_002176_06	euxassay_002176_07	euxassay_002176_08	euxassay_002176_09

euxassay_002176_10	euxassay_002176_11	euxassay_002176_12	euxassay_002176_13	euxassay_002176_14

euxassay_002176_15	euxassay_002176_16	euxassay_002176_17	euxassay_002176_18	euxassay_002176_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19361_wholemount_strong.wlz

19361_wholemount_moderate.wlz

19361_wholemount_weak.wlz

19361_wholemount_possible.wlz

19361_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19361_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 11 12 13 14 15 moderate expression: see section 16

meckel's cartilage

strong

regional

strong expression: see section 02 03 04 05 06 13 14 15 16

basioccipital bone

strong

regional

strong expression: see section 01 15 16 17 18 19

temporal bone

strong

regional

strong expression: see section 18 19

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5509
Entity Detected:	Sdc2, syndecan 2 ( MGI:1349165)
Sequence:	sense strand is shown >T5509 CGCCGCCGTTCGCCTTTCGGCATCCTAAACTTGAGCGGCAGCTCGGTTTCAACCCATCGACTGCTTGCTT CAAATCAGACAGCATTGCGACCCAGACACCGGAGTCCGCCGAGTGAAAGCACAACGCTGCCCTGTAGGAC CAGACCAAGAAAACAGCCTCGCGAAGCAGCAACTCTGGGTTGGGAGTACAGAAGCCAACAAGTGAGAAGG CGCCGCGTTTCCGGGGCGCAGGGAGAACGCTAGAGCAGGCGCCAGAGAAGACAGCTAGAGCTCGGAATCG GAGCCCAAACCTCTCTCCTGGAGGCGGCTCAGCTTCTTTTTTTTCTAGGCCCTCTTAGGCTTGCTCTGGG CTTCCTTTTATCCGGGTAGGAGCCACATCCCTGGGGAATATGCAGCGCGCGTGGATCCTGCTCACCTTGG GCTTGATGGCCTGTGTGTCCGCAGAGACGAGAACAGAGCTGACATCCGATAAGGATATGTACCTTGACAA TAGCTCCATTGAGGAAGCTTCAGGAGTATATCCTATTGATGATGATGACTATTCTTCTGCCTCAGGCTCA GGAGCTGATGAAGACATAGAGAGTCCAGTTCTGACAACATCCCAACTGATTCCAAGAATCCCACTCACTA GTGCTGCTTCCCCCAAAGTGGAAACCATGACGTTGAAGACACAAAGCATTACACCTGCTCAGACTGAGTC ACCTGAAGAAACTGACAAGGAGGAAGTTGACATTTCTGAGGCAGAAGAGAAGCTGGGCCCTGCTATAAAA AGCACAGATGTGTACACGGAGAAACATTCAGACAATCTGTTTAAACGGACAGAAGTTCTAGCAGCCGTCA TTGCTGGTGGTGTGATCGGCTTTCTCTTTGCCATTTTCCTCATCCTGCTATTGGTGTACCGCATGCGGAA GAAAGATGAAGGAAGCTACGACCTTGGAGAACGCAAACCATCCAGCGCAGCTTACCAGAAGGCACCCACT AAGGAGTTTTATGCATAAAGCTCCCATTTAGTGTCTCTATTTAGGAGAGCACTGAACTTTTCAAAATAAA GCTTTGGCATAGAATAATGAAGATCTTTGTTATCTGTTTTGTTCATTACAGAGCCATACTGGCCCTTTAA TGATGAGAATTCCATTGTATTTAAATTTTTTCGTGTATTTCTTTAGAATGACATAAAAGTAAAAATCTAA CATCTGCAGTGTTCTGTGAATAGCAGTGGCAAAATATTATGTTATGAAAACCCCTGACATTCATGGAATT GATTTGAACATCTATGTGCAAATACAAAATGATTGTGTTTGTCCTCTGGTTCAAAGATGACTGCTACTCC CCCTTGTCAGCAAGTCTCCAGTTAACCTTACTGAGTTGGTCTTCATTTATTTATCTCTTGTCCCTCTTCT CTACCCTCCCTTCTTGTCACCTTTCTTAGAAACAAAACCTTATGCCTTTTGTAGCTGTCATGGTGCAATT TGTCTTTGAATGATTACAATAATGGTAATTTAGTGTATATGTGATTTTTCTCAAATATGTTAACCTTAAC CTCCACTTTATATAATGTTTTTAAATATCAGACTATCCATTTTATGCTTGCTTTAAATTTCATTACTTGT AGCTTCAGGCAGATTTGCAAAGGCAAATTAATGTGTAAAATTGGATTATTACTACGAAACTGTTTAGTCC TAGCTATCTTCTTTTGGGAGGATTTGACATAACGGACAAGCCTCAGCAAACCCAAAGATGCTAACAGTAT TTTGAGAAGTTGCTGCAGATTCCTCTGGCCACTGTACTTGTTAATTTCTTGTCATTTGAAGGTACGAGAA AGAGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6395969 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6395969 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19364 same embryo
	EMAGE:19359 same embryo
	EMAGE:19360 same embryo
	EMAGE:19362 same embryo
	EMAGE:19363 same embryo
	EurExpress:euxassay_002176 same experiment
	MGI:4827919 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source