EMAGE mouse gene expression spatial database

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EMAGE:19362

Srpx2 sushi-repeat-containing protein, X-linked 2 ( MGI:1916042)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002179. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002179_01	euxassay_002179_02	euxassay_002179_03	euxassay_002179_04

euxassay_002179_05	euxassay_002179_06	euxassay_002179_07	euxassay_002179_08	euxassay_002179_09

euxassay_002179_10	euxassay_002179_11	euxassay_002179_12	euxassay_002179_13	euxassay_002179_14

euxassay_002179_15	euxassay_002179_16	euxassay_002179_17

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19362_wholemount_strong.wlz

19362_wholemount_moderate.wlz

19362_wholemount_weak.wlz

19362_wholemount_possible.wlz

19362_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19362_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 11 12 13 14

meckel's cartilage

strong

regional

strong expression: see section 01 02 12 13 14 15 16

lower jaw incisor

strong

regional

strong expression: see section 03 04 05 06

upper lip

moderate

regional

moderate expression: see section 10 11

upper jaw incisor

strong

regional

strong expression: see section 03 04 06 07

orbito-sphenoid

strong

regional

strong expression: see section 01 02 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6031
Entity Detected:	Srpx2, sushi-repeat-containing protein, X-linked 2 ( MGI:1916042)
Sequence:	sense strand is shown >T6031 CCGACTGACTGATGAGAGGAGCAGAGAGCAAAGAAAAAGAAGAGAGATGAACGAGACCACAAAAATCATA AAATAAAAAGCAGATGCATGTTCCTGCTCTTTTCAAAGCTTCCAGTAGAACGATAGCTCCCTCCGATGTC ATATGCCTGCTTCCTCTCACTTTTGGGAGTTCATAGCTGGTTCTGCTCTGAAAGTCATTCCCCTTTATCC TGGTACTTCTGGCCACATAGCCCTAGTCTTGTCTTCTGAAGCTTCCCTGTCGACGATGATGACCAGTCCA CTGACTCAGAGAGGTGCTCTCTCACTGCTGCTCCTCCTAATGCCCGCAGTGACACCAACATGGTACGCAG GCTCAGGTTACTCTCCAGATGAAAGCTACAATGAAGTATATGCAGAAGAGGTCCCCGCTGCCCGTGCCCG TGCCCTGGACTACAGAGTCCCCCGATGGTGTTACACATTGAATATCCAGGATGGAGAAGCCACATGCTAC TCACCAAGGGGAGGAAATTATCACAGTAGCCTAGGCACACGTTGTGAGCTCTCCTGTGACCGGGGCTTTC GATTGATTGGACGGAAGTCAGTGCAATGTTTGCCAAGCCGGCGTTGGTCTGGAACTGCCTACTGCAGGCA GATAAGGTGCCACACACTGCCATTCATCACTAGTGGCACCTATACGTGCACCAATGGAATGCTGCTTGAC TCCCGTTGTGACTATAGCTGTTCTAGTGGCTACCACCTAGAAGGAGATCGCAGCCGCATCTGCATGGAAG ATGGGCGATGGAGTGGAGGCGAGCCTGTATGTGTAGACATAGATCCACCCAAGATCCGTTGTCCTCACTC TCGTGAGAAAATGGCAGAGCCAGAGAAACTAACTGCTCGAGTATACTGGGACCCACCCTTAGTGAAAGAT TCTGCTGATGGTACCATCACCAGGGTGACACTTCGGGGTCCAGAGCCTGGTTCTCACTTCCCTGAAGGAG AACATGTGATTCGTTATACTGCCTATGACCGAGCCTACAACCGGGCCAGCTGCAAATTCATTGTAAAAGT ACAAGTGAGACGCTGTCCCATTCTGAAGCCACCACAGCACGGCTACCTCACCTGCAGCTCAGCGGGGGAC AACTATGGTGCCATCTGTGAATACCACTGTGATGGTGGTTATGAACGCCAGGGGACCCCATCCCGAGTCT GCCAGTCAAGTCGACAGTGGTCAGGAACACCACCTGTCTGTACTCCTATGAAGATTAATGTCAATGTTAA CTCGGCTGCTGGCCTCCTGGATCAGTTCTATGAGAAACAGCGACTCCTCATAGTCTCTGCTCCTGATCCC TCCAATCGGTACTACAAAATGCAAATCTCTATGCTGCAGCAATCCACCTGTGGACTCGACCTGCGGCATG TGACCATTATTGAGCTGGTGGGACAGCCACCTCAGGAGGTGGGGCGCATCCGGGAGCAACAACTATCAGC AGGCATCATTGAGGAGCTCAGGCAATTCCAGCGCCTCACTCGTTCCTACTTCAACATGGTGTTAATTGAC AAGCAGGGTATTGACCGGGAACGCTATATGGAACCTGTCACCCCTGAGGAAATCTTCACATTCATCGATG ACTACCTGCTGAGCAATGAGGAGCTGGCACGGAGAGTGGAGCAGAGGGACCTATGCGAGTGACCTTGAGT CAGGGTGTGGCTGAAGCACCTGTCCTAGGGAGCTTAAACTAAGGAGGAAATGTCGTGTCCTCCCCCCCCC ACACACACACACACACACTGTTGGGAGCAGCCCTCGGGTGGGGGTGAGGTTTGCCAAATCTAGGACAGTT CCAGGCAGCATTTTAGGGCAGAATATTCATTTCCCTAAGCAAGTCTCCACTTCGGGTAGCACTGGAGGAG CCTATGAAACTGAGGACATGCCCTGGGTGGTGAGTTGTAGACCGAAGGCCTTCTTCACCTGCCTGGAGCC TCTCTCTCTAGACTCTTCCCAAAGCCTTTCAGATAAGTAAATACCAAATTCATTCCTTTAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:3488526 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3488526 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19364 same embryo
	EMAGE:19359 same embryo
	EMAGE:19360 same embryo
	EMAGE:19361 same embryo
	EMAGE:19363 same embryo
	EurExpress:euxassay_002179 same experiment
	MGI:4828449 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source