EMAGE mouse gene expression spatial database

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EMAGE:19389

Plekhh3 pleckstrin homology domain containing, family H (with MyTH4 domain) member 3 ( MGI:2384950)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006078. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006078_01	euxassay_006078_02	euxassay_006078_03	euxassay_006078_04

euxassay_006078_05	euxassay_006078_06	euxassay_006078_07	euxassay_006078_08	euxassay_006078_09

euxassay_006078_10	euxassay_006078_11	euxassay_006078_12	euxassay_006078_13	euxassay_006078_14

euxassay_006078_15	euxassay_006078_16	euxassay_006078_17	euxassay_006078_18

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19389_wholemount_strong.wlz

19389_wholemount_moderate.wlz

19389_wholemount_weak.wlz

19389_wholemount_possible.wlz

19389_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19389_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6066
Entity Detected:	Plekhh3, pleckstrin homology domain containing, family H (with MyTH4 domain) member 3 ( MGI:2384950)
Sequence:	sense strand is shown >T6066 GGGAGCGCAGCGCGCGCCGCCCGGGCCCCGGCCACCGGACGCCCTACCGGACACCACCCTCCTTGGAGCT TGGACAACCGCCCACCACGATTGCTGCACCAGACACTGCTCCTACAGAGCTGGACACTACAACGAACACT ACTTCCCAGCTCCGGACATTGCTCCCGGCTCCCTTCCCCCGGCCCTGGACGCTATCTCCCCCCTCCTCTG CCCGGGCCCAGACAGCGAGCCCCCAGCGGGCTCTGGTGATAGTCCTTTTGCACCTCAGCGCTGGACACTG CCTCCTTCCGGCCCCCTTCTTTCCTCTCGTCGCACCTCTTCTCTACCCAGTTCGGGTCGGAGTACCTCCA GTTTCTCAGATTCCCTGCTGCTCAGGCAACACTGCCTGCGCCCCCATTCCCAGGCTGCTCGCCCGGATGC CTCTCCCCGGGGGATTGTGGTGGCTTCTCTGCTGCCGTCGAGGCTTCACTCTTCTGCACCGGGACTACGG GGACGGCGAGCTTAGCGGGGACGGGGACGAAGACGAGGACGACGAGACCTTTGAGCTAAGGACCCCGAGT CCAGCGGGCGGCGGGAGGGGTTCCCTGGACGTTACTCTGACTCAGCCAACAAGGAATGGGCCCATCTCAG ACAGACTGCAGGGCTGGGAAGAGACCTGGAGCCTCATCCCAGACAAGGGACTTCCGGAAGAGGACCCTGA CATCATTGTGAAAGGTTGGCTGTACCGGGAACCTCGTGGAGGAGGGGCAAGGCCATGGTTACTCCCACGC AGAGCCTGGTTTGTGCTCACCAGAGATTCCCTGGACCAGTTCAGCAGCAGCGGGAAGGGGGCAAGGCGAC TCGGAAGCCTGGTCCTCACAAGCTTGTGCTCAGTGACTGGGCCGGAGCGTAGGCCCAAGGAGACTGGTCT GTGGTCAGTCACTGTGTCTGGCCGGAAACACAGCATCCGCCTCTGCTCTCCGCGCCAGGCAGAGGCAGAG CGCTGGGGGGTAGCACTGCGGGAGGTGATTGCCTCCAAAGCTCCGCTGGAGACCCCTACCCAACTGCTGC TCAGGGACATCCAGGAGAGCTGTGGAGACCCAGAAGCAGTGGCCCTCATCTACCGGCGGAACCCAATCCT GAGGCACACCAGTAGTGCCTTGTATGCCCCGCTCTTGCCCCTGCCCTATGAAGTCAGCGCTCCAGGTCCA GGCTACGCACCCTTACGAGAGGAGGCAGTGAGGCTGTTCCTGGCACTGCAGGCGCTGGAGGGGGCACGGC GCCCAGGGCCCCTGATGCAGGGTGTGCTCCAGACCTGCCGGGACCTGCCTGCACTCCAGGACGAACTTTT CCTGCAGCTGGCTAAGCAGACCTCAGGTCCTGCAGGTCCCCCTGGGCTCCCTGCCACTCAAGATCCTGCA ACCCTGCGATACTGGCAGCTTCTCACTTGCATGAGCTGCACCTTCCGGCCGGGGGGAGCTGTCCGAGGGC ACCTCCTAGGGCATTTGGAAAGGACGGAGCAGGCACTCCCAGACTCAGAACTGGCAGAATATGCACGCTT CATCCGGAAGGCTCTGGGCCGTACACGGGGCCGAGAGCTGGTGCCTTCACTAGCAGAGATTTCCGCACTG AGCAGAAGGCAGGAGCTGTTGTGTACTGTGCACTGTCCCGGAGCTGGAGCCTGTCCTGTGTCTATCGACT CCCACACTACAGCGGGAGAGGTGGCTCGAGAGTTGGTGGGCAGGCTAGGTTTGGCCCGGAGCCGGAACGC ATTCGCTTTGTATGAGCAGCGAGGAGCCCAGGAGCGAGCCCTGGCTGGGGGGACCCTCGTGGCCGACGTG CTCACCAGGTTTGAGAATTTGACTTCGGAGGAAGCCGGACTGGAGGACTCGCCGGACTGCGGGTGGAGAC TGTGTCTTCGTCTTCACGGACCTCTGCACCCAGAAGGGCTATCTCCAGAGGGTCACGAATTGCCTTTCCT CTTTGAGCAGGCTCACGCTCTGCTGCTGCGCGGCCGGCCGCCCCCACCCGAGGACACGCTGCGCGCCCTG GCGGCGCTGCGCCTGCAGAGTTTGCACCGGGACTTTTCCCCGCGGGGACCCCTGCCGCTCCTGGACCGCC TGCTGCCACCCCCCATCCCGCCGCGCGAACAACCGCCGTGCCCTACCCGAAGGCCACCGCCCTCCGCTGC CCTGCTGGCCGGGGCGCTCTGGAGCCCCGGCCTGGCCAAGAGGCGGGCCGAGCGTGCCCGGCGCGGCGGA ACCGGCCGCTCTACGGGAAGCACGGCCCAGGTGGGAGGTGGCGGCGCCAGCACGACGGCTGCGGTGCTGG GCGGCTGGAAGCGGCTGCGGGGCATGGGCCAAGCTGAAGCCATGGCTGCCTACCTGGCTCTAGCGGCGCA GTGTCCGGGGTTCGGCGCTGCTCGGTACGACGTTCTGGAGCTGAGCACGGAGCCTGGTGGAGGTGCTCCA CAGAAGCTCTGCCTGGGTCTGGGAGCAAAGGCCATGTCGCTCTCCAGGCCTGGTGAGACAGAACCCATCC ACAGTGTCAGCTATGGTCATGTGGCCGCCTGCCAGCTAATAGGCCCTCACACCTTAGCACTGAGGGTGGG CGACAGCCAGCTCCTCCTGCAGAGTCCCCAGGTGGAAGAGATCATGGAGCTGGTGAATGCCTACTTGGCC AACCCCTCCCCCGAGAGGCCCTGCCGCAGCGGCAGCAGCTCTGGTCCTCCAAGCCAAGACCTGCCGGACA CCTCCCCTCCCAGCCAGCACCAAGTTCTGGAAGAGCCCCAGGGACAGTCTGGCTGCTTGAAGCAGCTGCA GGACTAAGCCTGCCAAGAGGTCAGGACTTTTCTTTTGCCTCCAGCCTGAGACCGGACTGCTCTGAGATTT GAGGCAACCATGGACCCTTTTTTTGGCCCTGCAGGGACAGGGTACACCCACACCCGAGGGCAGCAATGGG TGTGGACAGTTTTCTAGTTTGTTTCTTAATTTATTTGTAGAAGAAAAAAAATCGTTATTTACACAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3490992 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3490992 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19392 same embryo
	EMAGE:19394 same embryo
	EMAGE:19393 same embryo
	EMAGE:19390 same embryo
	EMAGE:19391 same embryo
	EurExpress:euxassay_006078 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source