EMAGE mouse gene expression spatial database

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EMAGE:19392

Khdrbs1 KH domain containing, RNA binding, signal transduction associated 1 ( MGI:893579)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004797. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004797_01	euxassay_004797_02	euxassay_004797_03	euxassay_004797_04

euxassay_004797_05	euxassay_004797_06	euxassay_004797_07	euxassay_004797_08	euxassay_004797_09

euxassay_004797_10	euxassay_004797_11	euxassay_004797_12	euxassay_004797_13	euxassay_004797_14

euxassay_004797_15	euxassay_004797_16	euxassay_004797_17	euxassay_004797_18	euxassay_004797_19

euxassay_004797_20	euxassay_004797_21	euxassay_004797_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19392_wholemount_strong.wlz

19392_wholemount_moderate.wlz

19392_wholemount_weak.wlz

19392_wholemount_possible.wlz

19392_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19392_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6060
Entity Detected:	Khdrbs1, KH domain containing, RNA binding, signal transduction associated 1 ( MGI:893579)
Sequence:	sense strand is shown >T6060 GCTGTCGCTCCTGTTCTTCCACCCCCGCCAACCGCCGCTCGGGCCTCAGCTGCCGCCGCGTCGCTTCCTC GCTCGTTCGCTCGCCACCCATCATCCCCGATGCAGCGCCGGGACGATCCTGCCTCGCGCCTCACCCGGTC CTCGGGCCGCAGCTGCTCCAAGGACCCGTCAGGTGCCCACCCCTCGGTGCGTCTGACCCCGTCTCGGCCG TCGCCGCTTCCTCACCGGCCCCGGGGAGGGGGAGGTGGGCCCAGAGGAGGCGCTCGGGCCTCGCCCGCCA CGCAGCCGCCGCCGCTGCTGCCTCCCTCCACCCCGGGTCCCGACGCGACGGTGGTGGGTTCCGCGCCGAC CCCGCTGCTGCCCCCGTCAGCCACAGCCGCGGTCAAGATGGAGCCGGAGAATAAGTACCTGCCTGAACTC ATGGCCGAGAAGGACTCGCTCGACCCGTCCTTCACTCACGCCATGCAGCTGCTGTCCGTAGAAATTGAGA AGATTCAGAAGGGAGAGTCAAAAAAAGATGACGAGGAGAATTATTTGGATTTATTTTCTCATAAGAACAT GAAGCTGAAAGAACGCGTGCTGATACCTGTCAAGCAGTATCCAAAGTTCAATTTTGTGGGGAAGATTCTT GGACCACAAGGAAATACAATCAAAAGACTCCAGGAAGAGACTGGTGCAAAGATCTCTGTCTTGGGGAAGG GTTCAATGAGAGACAAAGCCAAGGAGGAAGAGTTGCGCAAGGGTGGAGACCCCAAATATGCCCATTTAAA TATGGATCTGCATGTCTTCATTGAAGTCTTTGGACCCCCGTGTGAAGCTTATGCTCTTATGGCCCATGCT ATGGAAGAAGTCAAGAAGTTCCTAGTACCAGATATGATGGATGATATCTGTCAGGAGCAGTTTCTAGAAT TGTCCTACTTGAACGGAGTACCTGAACCCTCTCGTGGTCGTGGGGTATCTGTGAGAGGACGAGGAGCTGC CCCTCCTCCTCCACCTGTTCCCAGAGGACGTGGTGTTGGACCACCTAGAGGAGCTTTGGTTCGTGGAACC CCAGTGAGAGGCTCCATCACCAGAGGTGCCACTGTGACTCGAGGAGTGCCACCCCCACCTACTGTGAGGG GTGCTCCAACACCAAGAGCTCGGACAGCTGGGATTCAGAGAATACCTTTGCCTCCCACACCTGCACCAGA AACATACGAAGATTATGGATATGATGATACATACGCAGAACAGAGTTACGAAGGCTATGAAGGCTATTAC AGCCAGAGTCAAGGGGAGTCAGAGTATTATGACTATGGACATGGGGAGCTCCAAGATTCTTACGAAGCCT ACGGACAAGATGACTGGAATGGGACCAGGCCATCACTGAAGGCTCCTCCAGCTAGGCCAGTGAAGGGAGC ATACAGAGAGCATCCATATGGACGTTATTAAAAACAAACAGGAGGGAAAAATATCAGTTATGAGCAAAGT TGTTACTGATTTCTTGTATCCCAGGATTCCTGTTGCTTTACCCACAACAGACAAGTAATTGTCTAAGTGT TTTTCTTCGTGGTCCCTTTCTTTTCCCCACTTCCTCCATTCTTAACTCTGCATTCTGGCTTCTGTATGTA GTATTTTAAAATGAGTTAAAATAGATTTAGGAATATCGAATTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3490667 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3490667 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19394 same embryo
	EMAGE:19393 same embryo
	EMAGE:19390 same embryo
	EMAGE:19389 same embryo
	EMAGE:19391 same embryo
	EurExpress:euxassay_004797 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source