EMAGE mouse gene expression spatial database

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EMAGE:19394

Mxd4 Max dimerization protein 4 ( MGI:104991)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004800. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004800_01	euxassay_004800_02	euxassay_004800_03	euxassay_004800_04

euxassay_004800_05	euxassay_004800_06	euxassay_004800_07	euxassay_004800_08	euxassay_004800_09

euxassay_004800_10	euxassay_004800_11	euxassay_004800_12	euxassay_004800_13	euxassay_004800_14

euxassay_004800_15	euxassay_004800_16	euxassay_004800_17	euxassay_004800_18	euxassay_004800_19

euxassay_004800_20	euxassay_004800_21	euxassay_004800_22	euxassay_004800_23	euxassay_004800_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19394_wholemount_strong.wlz

19394_wholemount_moderate.wlz

19394_wholemount_weak.wlz

19394_wholemount_possible.wlz

19394_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19394_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 18 19 20 21 22 23

mandible

moderate

regional

moderate expression: see section 03 04 05 06 07 08 15 16 17 18 19 20 21 22

maxilla

moderate

regional

moderate expression: see section 04 05 06 07 08 09 16 17 18 19 20 21

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 21 22 23 24 weak expression: see section 06 07 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6063
Entity Detected:	Mxd4, Max dimerization protein 4 ( MGI:104991)
Sequence:	sense strand is shown >T6063 GGCAGAGGGTGGCGGCCCAGCGCTGGAGTCCGCGAGTCGGGCTGGTGTGCCGCCAGGCGCGGGGCGTGCG GCGAGCAGAGCCGGCAGGAGCGCGCCGGGCAGCCGCCGCCGCCGCCGTCCCGTGCGCGGGCGGCAGGATG GAGCTGAACTCTCTGCTGCTGCTGTTGGAGGCGGCCGAGTACCTGGAGCGTAGGGACCGAGAGGCCGAGC ACGGCTACGCCTCCGTGCTGCCCTTCGACGGCGACTTCGCAAGGAAGAAAACAAAGACGGCCGGCCTGGT GCGCAAGGCCCCGAACAACAGGTCTTCACACAACGAACTAGAAAAGCACAGACGAGCTAAACTCAGGCTC TACTTGGAGCAGCTCAAGCAGCTGGTACCCCTGGGCCCTGACAGCACACGTCACACTACTCTGAGCCTTC TGAAGCGTGCTAAGATGCACATCAAGAAACTGGAGGAGCAGGACCGCAGGGCACTGAGCATCAAGGAACA GCTTCAGCGGGAACATCGCTTCCTAAAGAGACGTTTGGAGCAGCTATCAGTGCAGAGTGTAGAGCGTGTG CGCACAGACAGCACTGGCTCTGCTGTGTCCACAGATGACTCAGAGCAAGAGGTGGATATAGAGGGCATGG AGTTTGGCCCTGGGGAGCTGGACAGTGCTGGCAGCAGCAGTGATGCCGATGACCACTACAGCCTACAGAG CAGTGGCTGCAGCGACAGCAGCTATGGGCACCCTTGCAGGCGGCCTGGCTGCCCTGGCCTTTCGTAGGCT CCATGTCCTTTGCTTCTTGGCCCACCTGCCTGCTCACCTAGCCAAGCGTCTCAGCCTTCCAATCCTCCCT TGACTGAAGCCAGCCTCGTCATGGGCCTACTGCTGTGCCATTCTCGAAGCTACAGCTGCTGTCTGGTCTG CCTGCCTCTGCCTGCCTACCCTTCGGTCAGGACTCAAGTTGCCTGCCCCTTCTCCGCAGGGCTAGACCCC ATGCAGGGAAGCAGTGTCAGGCTCTTACATCCTGGAGTCCAGCGTAGCCACCCACAATCTGTTCTCAGAT TCCTAATCATTCCAGAAGTATTAAATATCATTTCTACAAATCTCGGCAGGATCCGTGTAGGGGCCTTAAT GACCACCATAGGGAGCTTGAACCCCAACATCGGATCCCAAGAGAAAGGAGTGGACTGAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:3490469 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3490469 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19392 same embryo
	EMAGE:19393 same embryo
	EMAGE:19390 same embryo
	EMAGE:19389 same embryo
	EMAGE:19391 same embryo
	EurExpress:euxassay_004800 same experiment
	MGI:4826519 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source