EMAGE mouse gene expression spatial database

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EMAGE:19397

Krt5 keratin 5 ( MGI:96702)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004799. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004799_01	euxassay_004799_02	euxassay_004799_03	euxassay_004799_04

euxassay_004799_05	euxassay_004799_06	euxassay_004799_07	euxassay_004799_08	euxassay_004799_09

euxassay_004799_10	euxassay_004799_11	euxassay_004799_12	euxassay_004799_13	euxassay_004799_14

euxassay_004799_15	euxassay_004799_16	euxassay_004799_17	euxassay_004799_18	euxassay_004799_19

euxassay_004799_20	euxassay_004799_21	euxassay_004799_22	euxassay_004799_23	euxassay_004799_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19397_wholemount_strong.wlz

19397_wholemount_moderate.wlz

19397_wholemount_weak.wlz

19397_wholemount_possible.wlz

19397_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19397_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

spotted

strong expression: see section 07 08 09 10 11

physiological umbilical hernia epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

strong

regional

strong expression: see section 05 06 07 08 09 20 21 22 23

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 04 05 06 07 14 15 16 17 18 19 20 21 22

conjunctival sac

strong

regional

strong expression: see section 01 02 03 04 05 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 17 18 19

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 13 14 15 16 17

naso-lacrimal duct

strong

regional

strong expression: see section 04 05 06 07 08 09 18 19 20 21 22 23

pharynx

strong

regional

strong expression: see section 08 09 10 11 12

esophagus

strong

regional

strong expression: see section 08

tongue epithelium

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17

lower jaw incisor

strong

regional

strong expression: see section 10 11 12 14 15 16

lower jaw molar

strong

regional

strong expression: see section 17

oral epithelium

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

upper jaw incisor

strong

regional

strong expression: see section 16

upper jaw molar

strong

regional

strong expression: see section 17 18

urinary system

strong

regional

strong expression: see section 10 11

bladder

moderate

regional

moderate expression: see section 10 11

trachea

moderate

regional

moderate expression: see section 09

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6492
Entity Detected:	Krt5, keratin 5 ( MGI:96702)
Sequence:	sense strand is shown >T6492 AGCGTCCTGTTGAACGCCGCTGACCTCGCCAGCACCTCCCCGCCACCAGCGCCCAGTGTTCTTGCTCCTC CAGGAACCATCATGTCTCGCCAGTCCAGTGTGTCCTTCCGAAGTGGGGGCAGCCGCTCTTTCAGCGCCGC CTCTGCCATCACCCCATCTGTCTCTCGTACGAGCTTCAGCTCAGTGTCCCGCAGTGGTGGTGGTGGTGGT GGCAGGATCAGCCTTGGGGGTGCTTGTGGAGCCGGGGGCTATGGCAGCCGGAGCCTGTACAATGTGGGGG GCTCCAAAAGGATATCCTACAGTTCTGGCGGAGGCAGCTTCAGGAACCAATTTGGTGCTGGTGGCTTTGG CTTTGGAGGAGGAGCCGGCAGTGGATTTGGTTTTGGTGGTGGAGCTGGTAGTGGCTTTGGCTTCGGTGGT GGAGCTGGCTTTGGTGGTGGCTATGGGGGAGCTGGCTTCCCGGTATGCCCCCCTGGAGGCATCCAAGAGG TCACCGTTAACCAGAACCTCCTCACCCCTCTGAACCTGCAAATCGACCCCACCATCCAGCGGGTCAGGAC TGAGGAGAGGGAGCAGATCAAGACCCTCAATAACAAGTTTGCCTCCTTCATTGACAAGGTGCGGTTCCTG GAGCAGCAGAACAAGGTCCTGGACACCAAGTGGGCCCTGCTGCAAGAGCAGGGCACCAAGACCATAAAGC AGAACCTGGATCCGTTGTTTGAACAGTACATTAACAACCTCCGTAGACAGCTGGACGGGGTTCTGGGGGA GAGGGGCCGCCTGGACTCAGAGCTGAGGAACATGCAGGACCTGGTGGAGGACTACAAGAACAAGTATGAG GATGAGATCAACAAGCGTACCACGGCTGAGAATGAGTTTGTGATGTTGAAGAAGGATGTGGATGCTGCCT ACATGAACAAGGTGGAGCTGGAGGCCAGGGTCGATGCCCTGATGGACGAGATCAACTTCATGAAGATGTT CTTTGATGCGGAGCTGTCCCAGATGCAGACACACGTCTCTGACACATCTGTGGTCCTTTCCATGGACAAC AACCGCAGCCTGGACCTGGACAGCATCATCGCTGAGGTCAAGGCCCAGTACGAGGACATTGCCAACCGCA GCCGAACAGAGGCTGAGTCCTGGTACCAGACCAAGTATGAGGAGCTGCAACAGACAGCTGGCCGTCATGG CGATGACCTTCGAAACACCAAGCACGAGATCTCTGAGATGAACCGAATGATCCAGAGGCTGAGATCTGAA ATTGACAACGTCAAGAAGCAGTGTGCCAACCTCCAGAACGCCATTGCTGAAGCTGAGCAACGCGGGGAGC TGGCTCTCAAAGATGCCAGAAACAAGCTGACAGAGCTGGAGGAGGCCCTGCAGAAGGCCAAGCAGGACAT GGCCAGGCTGCTGCGGGAGTACCAGGAGCTCATGAACACCAAGCTGGCTCTGGACGTGGAGATCGCCACC TACAGGAAGCTGCTGGAGGGCGAGGAGTGCAGGCTGAGTGGGGAAGGAGTTGGACCAGTCAACATCTCCG TCGTCACAAACAGTGTCTCTTCTGGCTACGGAGGAGGCAGCAGCATTGGTGTTGGCAGTGGCTTTGGCGG TGGCCTTGGCAGCGGCTTTGCTGGTGGCCTTGGCCCACGCTTCACCAGGGGCGGTGGGGGTCTTGGTTTG GGTAGCGGGCTAAGTGTGGGGGGCTCTGGCTTCAGTGCAGGCAGCAGTCAAGGAGGCATGAGCTTCGGCA GTGGTGGTGGCAGCGGCTCCAGTGTCAAATTTGTCTCCACCACCTCCTCCTCCCGGAGGAGCTTCAAGAG CTAAGAAGAGCCTGCTGCAAGACCCTGGTCCTCACGGGCAGCCCCAGGCCCACAGAGACTGCTTCTTTTA GGCAGTCCCCCAGGCCACGTTTCCTTTTTCTGGAGAGTAGTCTAGACCATGCCTGCTGCAGAACCACATG CTTTGGTTCCCGGAAGGGAACGAATCCCAACCTCTGGCCTCCCCATGGCTCAGTTCTACATTTGTGTTGC ACGTCAGCACCTATACATGTTCTTTGGTGACCCAGACCCCAAAATGTTGCAGAATGTAAGCCACCAAAGC AGAACCCCATCCCTACCCGGATACCCACCTAAATTCTGTCCTGGTTCTGACTTCCTCCAGAGTCTGTCCA ATAAAATGCTTTTATAATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3589013 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3589013 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19400 same embryo
	EMAGE:19396 same embryo
	EMAGE:19398 same embryo
	EMAGE:19399 same embryo
	EMAGE:19395 same embryo
	EurExpress:euxassay_004799 same experiment
	MGI:4825840 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source