EMAGE mouse gene expression spatial database

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EMAGE:19413

Tm9sf3 transmembrane 9 superfamily member 3 ( MGI:1914262)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004806. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004806_01	euxassay_004806_02	euxassay_004806_03	euxassay_004806_04

euxassay_004806_05	euxassay_004806_06	euxassay_004806_07	euxassay_004806_08	euxassay_004806_09

euxassay_004806_10	euxassay_004806_11	euxassay_004806_12	euxassay_004806_13	euxassay_004806_14

euxassay_004806_15	euxassay_004806_16	euxassay_004806_17	euxassay_004806_18	euxassay_004806_19

euxassay_004806_20	euxassay_004806_21	euxassay_004806_22	euxassay_004806_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19413_wholemount_strong.wlz

19413_wholemount_moderate.wlz

19413_wholemount_weak.wlz

19413_wholemount_possible.wlz

19413_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19413_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6499
Entity Detected:	Tm9sf3, transmembrane 9 superfamily member 3 ( MGI:1914262)
Sequence:	sense strand is shown >T6499 CCCACGCGTCCGGGGAGAAGCACTTCAAGGAGTTGAATTAGAATTTAGTGGCCTGGACATTAAATTTAAA GATGATGTGATGCCAGGCACTTACTGTGAAATTGACTTAGATAAAGAGAAGAGAGATGCGTTTGTGTATG CTATCAAGAATCACTATTGGTATCAGATGTACATAGATGACTTACCAATATGGGGTATTGTTGGTGAAGC AGATGAAAATGGGGAAGATTACTATCTTTGGACCTACAAAAAACTTGAAATAGGCTTTAATGGAAATCGA ATTGTTGATGTTAATCTAACTAGTGAAGGAAAGGTCAAGCTGGTTCCAAATACTAAAATACAGATGTCAT ATTCGGTAAAATGGAAAAAATCGGATGTAAAATTTGAAGATCGATTCGATAAATATCTTGATCCATCCTT TTTTCAGCATAGGATTCACTGGTTTTCAATTTTTAATTCCTTCATGATGGTGATCTTCTTAGTGGGATTA GTTTCAATGATTTTAATGAGAACTTTAAGGAAAGATTATGCCCGATACAGTAAAGAAGAAGAAATGGATG ACATGGACAGAGACCTAGGAGACGAGTATGGCTGGAAGCAGGTGCATGGAGATGTGTTCAGACCGTCAAG TCACCCTCTGATCTTCTCCTCCCTCATTGGCTCTGGATGTCAGATATTTGCTGTGTCTCTCATTGTTATT ATTGTTGCCATGATAGAGGATTTATATACAGAGAGGGGGTCAATGCTCAGCACAGCCATATTTGTCTATG CTGCTACATCGCCAGTGAACGGGTACTTTGGAGGAAGTCTGTATGCTAGACAAGGAGGAAGGAGATGGAT AAAGCAGATGTTTATCGGGGCATTCCTTATCCCAGCTATGGTGTGTGGCACTGCATTCTTCATCAACTTT ATAGCCATTTATTACCATGCCTCTAGAGCCATTCCTTTTGGAACAATGGTGGCCGTTTGTTGCATCTGTT TCTTTGTTATCCTTCCTCTAAATCTCGTTGGTACAATACTGGGTCGAAATCTGTCGGGTCAGCCCAACTT CCCTTGTCGTGTCAATGCCGTGCCTCGTCCCATCCCGGAGAAAAAATGGTTTATGGAGCCTGCAGTTATT GTTTGCCTGGGAGGAATTTTACCTTTTGGATCAATCTTTATTGAAATGTACTTCATCTTCACGTCCTTCT GGGCATATAAGATCTACTATGTCTATGGCTTCATGATGCTGGTGCTGGTCATCCTGTGCATTGTGACTGT CTGTGTGACCATTGTCTGCACATACTTCCTGCTAAACGCAGAGGATTACAGGTGGCAGTGGACGAGTTTC CTGTCTGCGGCGTCGACCGCAATCTACGTTTACATGTACTCCTTTTACTACTACTTCTTCAAAACCAAGA TGTATGGCTTATTTCAAACATCATTTTACTTTGGCTACATGGCGGTGTTTAGCACAGCCTTGGGGATAAT GTGTGGAGCAATCGGTTACATGGGAACAAGTGCCTTTGTTCGAAAAATCTATACTAATGTGAAAATTGAC TAGATATCTGAGACAACATGGAACTTCGGATCAGTTTCTCTTTCATGAGGGTGGAACTTGCACAGAAAAG AAGAATAATCGCAAGAAGAGATTTGGGCTTAACACTGGGTACTTTGTGGGTCTCTTTTGTGGATGGCTTC AAGTAACATCTATTTCCATTGATCCTAGGTTCTTACTGACTGCTTTCTCCAGCTGTTCACAGCAGATGCT TGGATTTTATGCAGTAGGCTTTACTACAGTACATGGCTAATCTTCCCCAAACTAGCTCATTAAAGATGAA ATAGACCAGCTCTCTTCAGTGAAGAGGACAAATAGTTTATTTAAAGCATTTGTTCCAATAAAATAACTAG AGGGAAACTTGGATGCTAAAATTCCATAAGTAGAAAATCTTCCTGGCACTTGGTGTTTCTATGTTATTGA AAGATGATGTTCCAGAAAGATTACTTTTTCTTTTATTTTTACTGCCATTGTTGATGTGTTGTGGGACATT TTTATATATTGAACCTGGGTTCTTTTTTGATCTGTTTTGGTCGTCCCTCCATTCAACTGCATCTTTTTTT TTTTAAAAACAAAATTAAAAGCTATTCAATTCTGCATGGAATATATCTGCTGTCATCTTAATCTTAGTTG GATCAACCTCATTTATTTATCTTAAAATTAACCAGCTTTCTCAATTCCATCCAAAGAAGATAACAGTATG AAAATAGAGGTGCACTCTCTATAATACAATCTACTGTACAGTTAGCAAGCAAAGTGTTAAATGGAGAAGA TGCTTGTTTTTATTAAACATTTGGAGATTTAGATAATTTACATTTTAACTGAATGTCTAAAGTGATTATC TTCCCCTCCCTCCCCCAAGTTAATCTTCCATCTTTGTTTTTGGGTTTCAATGAAGGCTTTACATAAGAAA TTATTAAACAAAACAAGGGGGGCAATAAATGTATATAACATTAATAATGTCGCGTAGGTGTAGGTTCCTG GATGCATTTGGATGTACAGACTGACTGGAGGACTGTATTGCGATGATGATTGGTGTAGAAACGTATGGGT TTGGGTGGCCTTTTACATCTTGCCTACCATTGCATGAAACATTGGGGTTTCTTCAAAATGTGTGTGTCAT ACTTCTTTTGGGAGGGGGTTGTTTTCTTCTTTTATGAAACTCCTACTGGAGCTAAATTTGTAATTTAGAA ACATTTAATTTTGTTAATCCTGTCTGGGACATTTAGGTAACATCTAAAGCATTATTGCTTTAGAATGTTC AAATAAAATTTCCTGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3589474 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3589474 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19418 same embryo
	EMAGE:19415 same embryo
	EMAGE:19416 same embryo
	EMAGE:19414 same embryo
	EMAGE:19417 same embryo
	EurExpress:euxassay_004806 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source