EMAGE mouse gene expression spatial database

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EMAGE:19416

Ghitm growth hormone inducible transmembrane protein ( MGI:1913342)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004837. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004837_01	euxassay_004837_02	euxassay_004837_03	euxassay_004837_04

euxassay_004837_05	euxassay_004837_06	euxassay_004837_07	euxassay_004837_08	euxassay_004837_09

euxassay_004837_10	euxassay_004837_11	euxassay_004837_12	euxassay_004837_13	euxassay_004837_14

euxassay_004837_15	euxassay_004837_16	euxassay_004837_17	euxassay_004837_18	euxassay_004837_19

euxassay_004837_20	euxassay_004837_21	euxassay_004837_22	euxassay_004837_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19416_wholemount_strong.wlz

19416_wholemount_moderate.wlz

19416_wholemount_weak.wlz

19416_wholemount_possible.wlz

19416_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19416_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal medulla

moderate

homogeneous

moderate expression: see section 06 07 08 13 14 15

thymus primordium

moderate

homogeneous

moderate expression: see section 09 10 11 12

submandibular gland primordium

moderate

regional

moderate expression: see section 05 06 07 08 13 14 15 16

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 13 weak expression: see section 14

pons mantle layer

moderate

regional

moderate expression: see section 08 16 not examined expression: see section 14

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 18

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 14 15 16 17 18 19 20

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 13 15

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 not examined expression: see section 13 14 15

liver right lobe

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6496
Entity Detected:	Ghitm, growth hormone inducible transmembrane protein ( MGI:1913342)
Sequence:	sense strand is shown >T6496 GACGCGTGGGCGCGCGGTTGCGGAGGAGCAGGCAGAGGGAGGTCGCACACTCTGAGTTTCGGTGACCCGG AAGGGAGCCCCGTGGTAGAGGTGACCGGAGCTGAGCATTTCAGATCTGCTTAGGTAAACCGGTGTATCGC CCACCATGTTGGCTGCAAGGCTTGTGTGTCTCCGGACACTACCTTCCAGGGTTTTCCAGCCCACTTTCAT CACCAAGGCCTCTCCACTTGTGAAGAATTCCATCACAAAGAACCAATGGCTCGTAACACCCAGCAGGGAA TATGCTACCAAGACAAGAATTAGGACTCACCGTGGGAAAACTGGACAAGAACTGAAAGAGGCAGCCTTGG AACCATCAATGGAAAAAATCTTTAAAATCGATCAAATGGGAAGGTGGTTTGTTGCTGGAGGAGCAGCTGT TGGTCTTGGAGCGCTCTGCTACTATGGCTTGGGAATGTCTAATGAGATTGGAGCTATCGAAAAGGCTGTA ATTTGGCCTCAGTATGTAAAGGATAGAATTCATTCTACTTACATGTACTTAGCAGGAAGTATTGGTTTAA CAGCTTTGTCTGCCTTGGCAGTAGCCAGAACACCTGCTCTCATGAACTTCATGATGACAGGCTCTTGGGT GACAATTGGTGCAACCTTTGCAGCCATGATTGGAGCTGGAATGCTTGTACACTCAATATCATATGAGCAG AGCCCAGGCCCAAAGCATCTGGCTTGGATGCTGCATTCTGGTGTGATGGGTGCAGTTGTGGCTCCTCTGA CGATCTTAGGGGGCCCTCTTCTCCTGAGAGCCGCATGGTACACCGCTGGTATTGTGGGAGGCCTCTCTAC TGTGGCCATGTGTGCACCTAGTGAGAAGTTTCTGAACATGGGAGCACCCCTGGGAGTGGGCCTGGGTCTT GTCTTTGCGTCTTCTCTGGGGTCTATGTTTCTTCCCCCTACCTCTGTGGCTGGTGCCACTCTGTACTCAG TGGCAATGTATGGTGGATTAGTTCTTTTTAGCATGTTCCTTCTGTATGATACTCAGAAAGTAATCAAACG TGCAGAAATAACACCCATGTATGGAGCTCAAAAGTATGATCCCATCAATTCGATGTTGACAATCTACATG GATACATTAAATATATTTATGCGAGTTGCAACTATGCTAGCAACTGGAAGCAACAGAAAGAAATGAAGTA ACCGCTTGTGATGTCTCCACTCACTGATGTCTTGCTTGTTTAATAGGAGCAGATAGTCATTACAGTTTGC ATCATCAGAATTCCTTGAGGTTTAGAAGATAGCCTGTCACCATGTTTAAAATGTGCAGTAATGCGACCCT TCAGGCATGCCTTTTCTTTTAGAAAATAAATGCAATAGATGTCTTCCAAATATAAAAAAAAAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3588758 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3588758 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19418 same embryo
	EMAGE:19413 same embryo
	EMAGE:19415 same embryo
	EMAGE:19414 same embryo
	EMAGE:19417 same embryo
	EurExpress:euxassay_004837 same experiment
	MGI:4825051 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source