EMAGE mouse gene expression spatial database

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EMAGE:19443

Pou6f1 POU domain, class 6, transcription factor 1 ( MGI:102935)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005193. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005193_01	euxassay_005193_02	euxassay_005193_03	euxassay_005193_04

euxassay_005193_05	euxassay_005193_06	euxassay_005193_07	euxassay_005193_08	euxassay_005193_09

euxassay_005193_10	euxassay_005193_11	euxassay_005193_12	euxassay_005193_13	euxassay_005193_14

euxassay_005193_15	euxassay_005193_16	euxassay_005193_17	euxassay_005193_18	euxassay_005193_19

euxassay_005193_20	euxassay_005193_21	euxassay_005193_22	euxassay_005193_23	euxassay_005193_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19443_wholemount_strong.wlz

19443_wholemount_moderate.wlz

19443_wholemount_weak.wlz

19443_wholemount_possible.wlz

19443_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19443_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

moderate

regional

moderate expression: see section 08 09 21 22 23 24 weak expression: see section 01 02 03 04 05 06 07

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 22 moderate expression: see section 07

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 19

trigeminal v ganglion

strong

regional

strong expression: see section 22 23 moderate expression: see section 04 05 06 07 08 09 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 08 18

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 22 moderate expression: see section 04 05 06 07 08 09 17 18 19 20 21

trigeminal v nerve

moderate

regional

moderate expression: see section 10 17

spinal cord

strong

regional

strong expression: see section 09 10 11 12 13 14 15

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 16

cervical ganglion

moderate

regional

moderate expression: see section 08 09 17

thoracic ganglion

moderate

regional

moderate expression: see section 14

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 14 15 16 17 18

metanephros

moderate

regional

moderate expression: see section 05 06 15 16 17 18 19 20 weak expression: see section 07 08 09

testis

moderate

regional

moderate expression: see section 05 06 18 19 20

tail skeleton

moderate

regional

moderate expression: see section 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8831
Entity Detected:	Pou6f1, POU domain, class 6, transcription factor 1 ( MGI:102935)
Sequence:	sense strand is shown >T8831 AGAAAGACTGCAAGAAGACTCAACCTCTGGTCCTCGAACCTACATGTCTACTGACCCAAGGGAGGGGGGG AATGAGAGATGTTGCATGTGCCAGCCCTTCAAGAATCAAGGGAATTGCAGCTGCCCCGTGCCCACTGCCC CCACTGGGGGCATTTTCGTGCCAGATCCCTTGGGACTGGAACTGTGCCGCGTCATGGCTAGGCGCTTGAG CACCTGTCTCTCTGCCTTGCTCCGTAGCAGCTGCCGTGCTGAACACCGCTCTCCCGACACCTGTACAAGC TGCCCCACCAATCCAGGCCTCCTCGCCCGCCCAGCCCCGGCCACCAGCTCAGCCCCAGCCACTGTTCCAG ACCCAGCCGCTGCTACAGACCACGCCTGCCATCCTCCCACAACCCACCGCTGCCACCGTTGCTGCCCCCA CACCCAAGACAGTGGACGCCACCCCGCAGATCACTGTCCAGCCTGCAGGCTTCGCATTTAGCCCAGGGAT CGTAAGCCGCTGCCCTCACCCAGGGCTGACGAGGGATGGACCAAAGCTTGGGCCAAGGGTGGAATGTCCC CCACAGTGGAGGACCAGCTCCTTGAACACAGCCATTCCCGGCACATCAGCAAAGATGGGCTCCCATTGAG ATCAGTGCAGCCTCCCTCGGGGGACAGACGCAGATCCTGGGCTCCCTCACTACAGCTCCAGTTATTACCA ACACCATTCCCAGCATGCCCGGGATCAGCAGTCAGATCCTCACGAATGCTCAGGGACAGGTTATTGGAGC ACTTCCGTGGGTAGTGAACTCAGCTAGCGTGGCCACACCAGCACCGGCACAGAGCCTGCAGGTCCAAGCC GTGACTCCCCAGCTCTTGTTGAATGCCCAGGGCCAGGTGATCGCAACCCTAGCCAGCAGCCCCCTGCCTC AGCCTGTGGCTGTCAGGAAGCCAAACACACCGGAGTCCCCTGCTAAGAGTGAGGTGCAGCCTATCCAGCC GACACAAGCCGTGCCCCAGCCTGCAGTAATCCTCACCAGCCCAACGCCAGCGCTCAAGCCGTCAGCTGCA ACTCCCATCCCAATCACCTGCTCAGAGACCCCAACCGTCAGTCAGTTGGTATCAAAGCCGCACACCCCAA GTCTGGATGAGGACGGGATCAACTTAGAAGAGATCCGGGAGTTTGCTAAGAATTTTAAGATCCGGCGGCT CTCCCTGGGTCTGACACAGACCCAGGTGGGCCAGGCTTTGACGGCGACAGAAGGGCCGGCCTACAGCCAA TCAGCCATCTGCAGGTTTGAGAAATTGGACATCACACCCAAGAGCGCCCAGAAGCTGAAGCCGGTTTTGG AAAAGTGGTTGATGGAGGCAGAGCTCCGCAACCAGGAAGGCCAGCAGAACCTGATGGAGTTCGTGGGCGG CGAGCCCTCCAAGAAACGCAAGCGGCGCACTTCCTTCACACCGCAGGCCATAGAGGCTCTCAATGCCTAC TTTGAGAAAAACCCCCTGCCCACCGGCCAGGAGATCACGGAGATCGCCAAGGAGCTCAACTACGACCGGG AGGTGGTGAGGGTCTGGTTCTGTAATCGACGCCAGACACTGAAGAACACCAGCAAGCTGAACGTCTTTCA GATCCCGTAGGGCTCAGTGTCAGCGTGTGCCGGCGCACTGTACTCTTCCCTCAGCAACCAGCCGAAGTCA CTGCGGTGACTGTACTGTGTGCAGCTGCGTTTGCCCTAGGTCGTGAGCCTCCACCTGTGCATGCGTGTCA ATGCCTGCCTCTTCTGCCCACATATGTCACACCCTGGGGAGGCCTGAGGGGCTGCATGAGAGCCTTAGGC TCTGGCTGGGCATGTGGAAGGAGGGGGTTTGTGGAGTCATAGAAAAGCACTTTGCTAAGGTGGTTTCTGA AGGGCGAGTTCCGGTTGGGAACCAGAAAGGCTCTGTCTTTGGGGCAGGGCTGTAGCAACTTTTGGGGACA ACTGTGTAGTAGCTCTTGGTTTTGGTGGCACCCTTTGCTCTTCCCTTCGCTCCTCTGTGGTTGTGCCCAT GTGACAACTGCAGCCTCACACTGCCTCCCTAACTCTGTCTATGGGCACACTGAACCCCTTAAAGGACAGA GGCGATAGAATCCAGAGAAGCATGGTGTGCTGCTCAGACTCACTGGCTGCCTTGATCTTGAGGGCAGAAG GTTCCCAAAGTGAAGCTGAGGGGTCGGATGCCCAAGGTCAGGAGCTGCGAGGTCTCTCCCCAGGTCCTGC TGGTGGTGGGTTTGTGCTCACTCGAAATGTTTTCTCCCGCAAACCAAGTTGGAAGGAAGCAAGAGTAAAC TCTCTCACCCTGGAGAGTCTTAGGTCTGTGGCAGGCCTGGAGTTTATAAAGTAAATGAGGACATCCACCC AGACTTATCTAAGTTTCCAGCTGTCTTCCAACACAAGAGCATGCTAGAGTTTGGGAAATTTTCCTCTGCC CACTTACACAGACAGCAGCATGGGGCACGGATTCCGACTGACTTTCCCCCCTCTCGTGCCCTTACCCATC TCAGTGCAGGACTGAGATGTCTTCAAAGGAGCCAATTGAACCAAAAGCCAGACATGGAGGAGCTGGCGTG GGAGGAAGACAGTATCTAAAGGGATGATGGTCCGGGGAGGTCTCTTGTTGAGGCTGACTTAACTCTAGTT TTGTTTGTAATTAAGTCTTGGAGATCTCCAGAGACGTCACTTTGTCCAGTTGTAGACCAGATCTGTCTTT GAGCAACCCTCCCCTCCACCCCCCGTCCTCATCCCTCTGGGGTGCTCCTCTGAGCCAACAGTGCCCAGGT CAGGACTTTCCCTGGGTAGGTGATGGCAATCCGGGACCTGGCATCACACCCAAGCCACTAAGCCAGACTT TGCAGCCACAGAGAGGCTGGCAGACTTTTCACTTCAGTGCATTGGCTGGGGAATGTGGCTCAGCGGGAGA GAGCTTGGCATGTATAAGGCTCCAGAGTCAGATTGTAGCACCAAGGGGAAACACTTTGCTTTCAAGAAGT CAGGAAAGAGCTAAGACGTTCTCTTGGATGACCCCTTTACAAGCTGTGGGCATCAACTGTCTCGATACTT TTGGGGTTGGTTTGTTGGCTTTTGTTTTGTTTTGTTTGTGATAGGATCACACTGTAGCCCAGGCTAACCT CAGACTTGGAATTGAAGGCGACCTGATCCTCCTGTCTCCCCCCACCTCCCAAGCACTGGGTCTGCAGTCG CGTGCCACTGTGCCGCTGTGCCTGGCTCTTCTCCACTCCACAGCCTTACTGAGCTTGCTCTCTGGATGAT GTTGACATCACGTGATCTACCCTGAAGCAATGACTCTCAAACATGGACATGATGCAGAGACACCAGGGCT CAGCTTTCTCTCTCGTACCCTGTTCCCTTTCCTGGTCCTCTTGGGTCCTGCAGTACTGGTGTGGAGGGAC CTCCTAGTAGCTCTGACAATGAGAGGAGGGCAGTTGTGTGCTGATCACACACACACACACACACCACAAG TCTGTCAGGTATAAGGAGACAAGTAGGTTGCTTTCCTGATCCTGCCTCCCAAGAAAGAATAATTCCCAGA GCGATTCTAGGTCATGTGATTATCATCCTGCTTCTGTGAGATGGAAGTCAGGAGGGGTCCTGTAAGAGAG CTTTCAGTAGGGATGCTTAGAGCCCTGAAGCTTATCAAGATGATGCACCTGAGAAAACTATATGCAGATC TGCAGCTTTTCTTGCTCAAAGCCTGCACTCAGGAGGTACTACACAGTCAGCCATGCTGTGGTCAGCCATG CTGTGGTCAGCCATGCTGTGGACATGGGAGAGGATGCCATGCAGTCTATGCATGACAGGCGGGGATAATC ATGGCCCACTAAATTAAGCCGACCACTCGTTCTCACCCCGTCCTTTGTCCAAATCTGTCTTCTCAAGGCT GGCCTAGAGTGAACCATTTTCTCCTGTGTTGGAAAGATATATTGGTCTTTCAGAACAGTACCATGTTTAC AGGCTGGACTTCTCAAGGAAGGGATTCAAGAGTATAAAGTGTACCCAAAGTGGGGTTCGCTTTGGCCTTT GGACTCTGCTCCCCAGCCTGGGCCACTGATCTACAGTCTTTGTTATTTTGGGGAGCATTAGACTCTAAAA AAAGTTTTAATAGCAGTTTTATCATGAAAGCACCTTCTCGGACGTAAACCCGCGCTCGATGGCTTTTTTT GCCCTGGAAAGGGGAGAATGGGAGGAAGTATTTCAACTGTGTGGTTGGTTTGTTTTAAGTGATGGGAAAC CAAGACACTCTGTGGCCCTGCTGAATCAGCTGCGCCCTTCGACCCTGCACTAACTATGGCACTTCACCCA AGCAAAGTGAACCGTGAACACACGTGAGTTTGGAATGAGAACACCTGATGTGTTTGTGTTCTTGAAAAGC CAGTCACCTTCCTATTCCCAGGACGCCTTTCCTGCCTGGTGGGCCAAATCCCGAAGTCCCAACATTCTCC TGGCACGGCAAGGGGCCACTGCCCACTGCTAGCTGGGGAGGAGGAAGGGGGAGTATTGCCGTAGCGTGTG AATAAACAGTATTTTTTTCTTTCGTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6410550 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6410550 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19441 same embryo
	EMAGE:19444 same embryo
	EMAGE:19442 same embryo
	EMAGE:19440 same embryo
	EMAGE:19445 same embryo
	EurExpress:euxassay_005193 same experiment
	MGI:4827343 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source